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Пікірлер
@IScreenshotNFTs 2 ай бұрын
Looks like you have edge effect when you seeded your cells. Next time seed, 25% of the well. Dumbos.
@tylersandberg1618 5 ай бұрын
Nice simple tips! Thanks for sharing!
@MabtechScience 5 ай бұрын
Thank you!
@hopeakpabli9381 6 ай бұрын
I learned a lot from this excellent presentation. Thank you.
@MabtechScience 5 ай бұрын
Glad it was helpful :)
@ashutoshmishra9630 7 ай бұрын
I am from India How could I join with you
@ashutoshmishra9630 7 ай бұрын
Hlo it's to.. good
@janinestokes4883 7 ай бұрын
Thank you
@sjlee9351 7 ай бұрын
This was a great talk. Thank you for sharing. I have one question. This might be a silly one, but what if you add only a peptide (for example, IFN-gamma) to the capture antibody-coated plate without cells? Can you still develop spots with detection antibodies and other reagents? What is the difference between adding the target antigen directly to the plate and adding cells that secrete the target antigen, which is then captured by the plate? I think they are the same in terms of binding targeting antigens to the capture antibody, but when the target antigen is only added without cells, there are no spots. Could you please explain the principle behind it?
@MabtechScience 7 ай бұрын
Thanks for you question! It sounds like an ELISA format may be better suited for your needs. In ELISpot, spots are formed since a high concentration of the analyte (say IFN-gamma) is secreted at the location of the responding cell that is bound by the capture antibody there. If you are adding a sample filled with IFN-gamma, it will be captured all over the plate which won't lead to spot formation. ELISA would work to detect secreted IFN-gamma in solution and give you the concentration of the analyte in your sample.
@Denise-ux4xd 10 ай бұрын
💉☠️
@AdYourCommentHere 10 ай бұрын
So nice to see the discussions between Cecilia and Christopher in the Q&A at the end!
@sumeyyealtunok2031 11 ай бұрын
After adding the cell and stimulus, it says to incubate for 18-48 hours. How long should I incubate for IFN gamma?
@MabtechScience 11 ай бұрын
Hi :) IFNg generally should produce nice responses after 18 hours, but if your sample is expected to contain fewer rare Ag-specific cells, increasing the incubation time up to 48 hours could be beneficial. We recommend that you optimize incubation time before using precious samples.
@hopeakpabli9381 6 ай бұрын
Please, I need a lot more of this for my thesis. Thank you
@nicolej8502 11 ай бұрын
Excellent presentation, thank you so much!!
@MabtechScience 11 ай бұрын
Thank you!
@AdYourCommentHere 11 ай бұрын
Very informative and a great introduction to ELISpot and FluoroSpot. The Q&A at the end proved very insightful with some great questions (always great to see B cell questions pop up!!)
@xiqiangwang6798 Жыл бұрын
How to set up a control group for B cell ELISPOT test and what is the positive control group
@MabtechScience Жыл бұрын
Hi, With B cell ELISpot, total IgG wells are used as a positive control for the assay. Wells are coated with anti-IgG mAbs. Negative controls could be wells coated with PBS. Cells from the same donor are added to wells coated with the antigen of interest, the positive control wells and negative control wells. Please see our post all about B cell ELISpot ( www.mabtech.com/knowledge-hub/b-cell-profiling-elispot-and-fluorospot ) and don't hesitate to reach out to us via our website if you have more questions!
@Sailorspoo 4 ай бұрын
We have a new webinar on this particular subject: kzbin.info/www/bejne/pZW8cqiCh6djr6csi=-N6ooNXMT0r2dSBu
@savitabains8622 Жыл бұрын
extremely descriptive video!!! very helpful.
@elvirapiten8039 2 жыл бұрын
is there any distributor in mexico?
@R.M.111 2 жыл бұрын
Thanks, 🌷
@XYZakiAM 4 жыл бұрын
The level of personality evident in this video has me convinced Mabtech is run by one single guy
@MabtechScience 4 жыл бұрын
Hehe, do not feed Christian's ego :) Mabtech is made up of many, many talented people. Thanks for nice feedback to the video!
@EuniceKiamba-y7x 5 жыл бұрын
Thanks a lot. Being the first time I am doing ELISpots, I found this tutorial very useful
@MabtechScience 5 жыл бұрын
Great to hear Eunice! This fills our hearts with happiness :)
@leukatz 5 жыл бұрын
great video! love the humour:)
@MabtechScience 5 жыл бұрын
Thx leukatz, we do our best!
@MabtechScience 5 жыл бұрын
Any questions or comments, please write them in here and we will respond ASAP!
@tomslocombe9758 5 жыл бұрын
Thank you!!
@MabtechScience 5 жыл бұрын
Thank you Tom!
@rwinkler4321 6 жыл бұрын
Your videos are amazing, thank you so much!
@MabtechScience 6 жыл бұрын
Thank you for the great feedback Robert!
@gingergeiger5296 7 жыл бұрын
Great video! Speaking voice is timed nicely, broad yet sufficiently detailed overview of software. Thank you!
@MabtechScience 7 жыл бұрын
Thanks alot, Ginger! Positive feedback like this gives us energy to produce more tutorial videos.
@ShampooWow 7 жыл бұрын
Awesome video! I like it
@MabtechScience 7 жыл бұрын
Thank you, Shampoo!
@traemeelhorizonte 8 жыл бұрын
Do you have tutoral video for a Fluorospot using this AID reader?
@MabtechScience 8 жыл бұрын
Dear David, we are working on such a tutorial. We hope to have it ready before the end of the year. However, if you email me directly at [email protected], I can send you a FluoroSpot Powerpoint Tutorial for the AID machine with nice print-screens. Could be helpful!
@traemeelhorizonte 8 жыл бұрын
Yes, that would be of great help. I will e-mail you shortly. Thanks!
@sumoyeebasu4771 Жыл бұрын
Is it still the same email to get the PowerPoint?
@sumoyeebasu4771 Жыл бұрын
@@traemeelhorizonte could you send on the PPT please?
@dhegazy59 9 жыл бұрын
very helpful , thank you very much
@MabtechScience 9 жыл бұрын
Thank you for the kind words Doha!