Eroglu Lab

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@alialmoussawi1068 Ай бұрын

you need to add something between the stacking and seperating so that your gel will be leveled. You can use isopropanol or butanol or simple DW, during the 30 - 60 mins wait time. Read Schagger protocol. Many issues.

@SewayPL 2 ай бұрын

This is a gorgeous learning resource thank you so much. I wish there were more demonstrations like this of lab protocols online.

@durmonov 2 ай бұрын

what is the ml of sulfuric acid?

@Afrin2005 3 ай бұрын

The music 🔥

@VenkataSiddharthPendyala 4 ай бұрын

Great explanation, but the bases are spelled wrong in a few images. It is Guanine, Cytosine, Thymine, and Adenine. You spelled them wrong in a few places.

@YEISONANDRESNAVIASAMBONI 4 ай бұрын

How many uL are 1 ug of DNA?

@ianlee5812 2 ай бұрын

I think this setup needs a DNA concentration that corresponds to 10 ng DNA in 20 µL liquid. So the DNA concentration is .5 ng/µL.

@aishakhalfan2760 5 ай бұрын

that tube labelled X contain what?

@KIEUMANHUYTMU 6 ай бұрын

Dear Mister I am quite confuse at 13:24 . Does it mean value = 0.488 must be in between 0.43 and 0. 52 of the y axis? Thank you

@kusumaKM-b4x 6 ай бұрын

Which paper did u refered for this experiment

@mattchang-mp5bu 6 ай бұрын

you are so cute！

@asma8322 7 ай бұрын

Thanks for u alot.. Egypt 🇪🇬

@mojzarea 7 ай бұрын

Thank you so much, Really helpfull

@tikizone2535 8 ай бұрын

Happy by seeing ❤

@2Sor2Fig 9 ай бұрын

This comment is 50% for the algorithm, 50% because I love how well you explained everything, so good. Even liked the music... Guess I just want to let you know your time is not wasted.

@manpreetkour7572 9 ай бұрын

how to purify protein from adherent cells ?

@danieloulhint7914 9 ай бұрын

This video is good but the music background is so disturbing

@remaahmed4894 Ай бұрын

I agree it completely distressed me 😢

@imranali-fm9sx 9 ай бұрын

How to prepare sample for maximum adsorption of BSA? Can you explain in detail

@DEDSE 9 ай бұрын

Thnx this video change my life for better ❤🙌🏻

@md.mostafijurrahman7561 10 ай бұрын

Great explanation and demonstrations

@amymedali 11 ай бұрын

Very clear procedure. Thanks a lot

@xiaohuilu8676 11 ай бұрын

you should never make a buffer in a cylinder!

@MolecularMedicine327 Жыл бұрын

AMAZING THANKS A LOT WHEN THERE IS NOONE TO HELP YOU ...EROGLU LAB IS ALWAYS TO SUPPORT..

@CoffeeKnows Жыл бұрын

when do you take out the rotor. If leaving it in the cylinder, it will affect the accuration.

@PooleySteen Жыл бұрын

If someone at your laboratory deliberately left 5 mL of T7 exonuclease gene 6 out at room temperature for one hour what would said to them?

@okyanusya4093 Жыл бұрын

Thank you, finally i understand total procedure.

@DocScoop7 Жыл бұрын

such a good video

@niloofarkh4779 Жыл бұрын

thank u very much

@leticiadamianodantas8107 Жыл бұрын

how much marker do you put on the gel?

@888Marilia Жыл бұрын

0.75*

@rawantej5139 Жыл бұрын

can you please explain how to prepare x and how to calculate the initial concentration of x in your exemple because i didnot realy get the calculation from the dilution

@dilipkulkarni2122 Жыл бұрын

Excellent. Very clear. Thank you.

@EramFatima-x6u Жыл бұрын

Why did your gel lanes appear blue after running SDS PAGE? I mean when you dismounted the gel, the lanes looked blue and not much difference after staining?

@jameelabduljalil25 Жыл бұрын

Thanks for sharing this.

@stephendick6189 Жыл бұрын

0.997 r^2 😟

@NasarKhan4U Жыл бұрын

can we check westernblot prepared samples with bradford? prepared sample mean samples with lemille buffer

@CR-uu1rr Жыл бұрын

Congratulations, I have never seen a tutorial as clear, detailed step by step and above all as complete as this one. Truly a lectio magistralis. Thank you, you are super.

@weaam__m0 Жыл бұрын

In phenol sulfuric acid test for carbohydrates should we use 80% phenol or 5%phenol?? There is reference write 5% some of them write 80%??

@peterlauridsen8403 Жыл бұрын

Propably the best SEC and AKTA Pure tutorial on KZbin! Thanks a lot

@zeeniyakirman2716 Жыл бұрын

Why do you take the concentration as 0.02 and 0.04 and so on?

@trendwithjojo Жыл бұрын

awesome explanatio😮

@Mr.DNArtist Жыл бұрын

❤❤❤

@Nicha-qu3nw Жыл бұрын

Thank for video please tell me how to sample extraction for used this method thank.

@facundomassolo4663 Жыл бұрын

Excellent, thank you!

@Tepmodify Жыл бұрын

I like video

@mehmoodulhassan4842 Жыл бұрын

sir I want it in written form can you provide it

@fatimahamel9610 Жыл бұрын

I want a written rapport for this technique (materils,method, objectif and conclusion)pleaaaaaaase

@vishalupadhyay416 Жыл бұрын

Worst video I ever seen 😭

@jahanhussain Жыл бұрын

AMAZING VIDEO!! thank you so much !! :))

@mohi7527 2 жыл бұрын

Really great. But may I ask what is X?

@georgenikolaras5609 2 жыл бұрын

Shouldnt the curve be starting from the beginning of the two axes (point 0,0)? Because if you have no absorbance you cant have concentration. And also, isnt the standard curve a version of Lambert-Beer's law? If so, we cant have equation of y=ax + b formation, we will have the formation y=ax. And the thing i cannot understand is why we do the standard curve. Cant we just use the bradford reagent and measure the complex absorbance and after that, using Lambert-Beer's law we find the protein's concentration? Please correct me if im wrong.

@paulinamontano5688 Жыл бұрын

As far as I understand for the Lambert-Beer's equation (A=C*e*l) you would need to use the extinction coefficient which is different for each reagent and is determined at a specific wavelength (defined by the solution you are measuring). If you observe, here they are using a 96 well plate here and is being read by the luminometer, I guess this is the instrument they will use to determine the concentration of proteins for their samples, but it can be done with a UV-vis. For the A=C*e*l normally you use an UV-vis spectrophotometer with a 1 cm cuvette, which allows you to set l=1 cm in the LB equation. (Or if you use a different pathway length I guess you would need to take that into consideration, for instance, if you are going to measure the concentration with a nanodrop, which you would need to adjust the value for "l", but anyway, I would strongly advise you to always use a cuvette to have more reliable results.

@talebinas9406 2 жыл бұрын

thank u

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