Anyone else have Chrome download issues for JR2730 and JR2731 R1 & R2? Sent an email to Minio regarding this. Resolved: Tried downloading on Edge and had no issues. Thx!

this is waaay to advanced for me

How to deal with so many NAs to have less NA without compromising the integrity of information?

How can I get this Rstudio theme?

Hello, first of all - I am so thankful for this series as I am about to perform my very first RNA-seq experiments. In order to comprehensively analyze my own datasets later on, I would like to learn with your dataset first, to follow the workflow as shown. I also tried to find your datasets but was not able to. Is there any chance you could upload them elsewhere so not only CU Boulder students have access? Again, thank you so much for this series and making it publicly available! Kind regards from Germany, Tom

Thanks for this, showing the most important skill in bioinformatics. “Huh… why didn’t that work?”

Thank you god bless

I'm starting this course today and I'm so psyched for it! I have a raw-data from both RNA-seq and MNase-seq and I really hope I can do the A-Z analysis by the end of this course (:

you have new sub

Multiple thanks for this vital video. Could you kindly share your email address with me for further inquiries?

Thank you very verry much, Professor John Rinn and your lab members for spending your precious time making these valuable and informative lectures sharing them for free via KZbin for poor students like us. I am a third-year undergraduate student major in Biotechnology in Vietnam and I feel very lucky to find your lectures to learn about Bioinformatics. I wish you all the best, Professor and lab members!

Love the new series. Thanks for the education.

Could you please design a case study involving ATACSEQ, RNASEQ and their integration. Like taking a paper and reproducing the results. It would be kind of unique way as far as I have watched most of the youtube channels.

Thanks a lot for this.

Hello! Thanks for the lesson. i tried to fond de raw data fastq files but is not possible. you can put any link to download this data to work locally? Thanks you for sharing your knowledge! Best regards!

This is on point 💯💯💯

Thank you!

Thanks for the video! Very clear explanation.

Hi, Thanks! I can run nextflow only in terminal's current session. I understand .bash_profile is in home directory. Inorder for nextflow be available in every session by default, Should .profile also be placed in home directory? Should .bash_profile also need path variables to be set?

Thanks

☹️ 𝘱𝘳𝘰𝘮𝘰𝘴𝘮

Hi All, I apologize we should have noted in this video that if you are not a CU student you can't connect to fiji-viz. The good news is all we are doing on Fiji Viz is running R-studio. Thus all you need to continue on from here is R-studio which can be downloaded for free here : rstudio.com/products/rstudio/download/#download The R-studio video will be the same set up and the main focus will be on your file paths. For example, where you set up your working directory should be the same place you cloned the github on your local computer or server. So in short once you have R-studio you will be able to continue with the ChIP-seq analyses. We start small with Pol II only - and for educational purposes that is really all you need to run. However if you have a computer with lots of hard drive space you can run most of the design file we have added in a .Rmd

I dont having the "Settings" menu on CLASS_2021 github page. Do I need an admission for that? But when I do what you do on bash concole I got the same results? Like when I type ls I have a folder called CLASS_2021 etc.

Thank you very much Professor. Excellent job.

When I type gencode_gr <- rtracklayer::import("data/gencode.v32.annotation.gtf") I got Error in open.connection(con, open) What should I do?