sir I have one doubt whether it is a plant DNA or other?

Sir kindly guide me. How can i link protein profiling with phytoremediation or metal analysis

Very nice video sir . I am clearly understand the techniques 👌.please sir upload another techniques .

Sir plz do more such practical videos .. keep uploading the practical videos for biotech students it really helps a lot.

Sir please comeback and keep making videos which demonstrates certain Biotechnology practicals.. it helps clear our basics and enhances our knowledge. Plz Keep on posting practical videos sir.

While loading the sample, some came out of the well. Should I press the tip further inside the glass plate? Won't it break the smaller glass plate?? I was using 10 microliter pipette.

While the video was helpful and appreciated the heavy accent and poor English made it difficult.

great efforts. thanks

Thank you so much sir 🙏

Highly informative sir..Thank you 🙏

Thank you so much Sir...i am grateful for your help

Please do a demo on western blot

Well explained! Thank you sir for this awesome video

Amazing, just the expression on her face when I gushed a crazy endless river was so exhilarating, I used what I mentioned last month and after the first 3 weeks or so my volume visibly doubled, I went ahead and go'ogled Jan Venstaker's Shooting Ropes and the intensity has been insane!

Sample kab add kiya???

If we have any personal queries or any sops we require. Can we ask your assistance?

I mean DO u DNA or RNA with loading dye have kept on normal paper/ cello tep??? I mean DNase RNase present na around normal environment?? DNA RNA to degrade ho sakta hai haina ??So I couldn't understand sir please help

I learned alot? RIGHT!

Why are you making poly acrylamide gel without gloves ????????????

sir it is really helpful..but want to ask you that the 4th step of the protocol which is incubate at 37C at 300 rpm had been missing in the video.... Please help me regarding this.

THANK YOU SO MUCH 🙏🙏🙏🙏

Sir make practical video on Elisa

Great work Dr singh Lecture well understood

In P plate, you said that only the plasmid has been put for the cell to get transformed. Your plasmid had GFP as well as antiobiotic resistance gene, the how will you explain about those colonies in P plate which are not flourescent and yet resistant to the antibiotic? If they have antibiotic resistivity, the why they don't have the fluorescence?

Super explanation sir👏

u r an excellent teacher if possible make a video on cytokine estimation by elisa

Hello sir. after ligation u incubate the reaction for 24 h but in our case we are using pcr clone jet kit and it's protocol suggest to incubate only 30 minutes

Nice session 👌👌

Thank you sir ji 🙏🙏

Thank you thank you so much sir

Thank you thank you so much sir

Please do more videos for molecular biology ,cell biology, microbiology practicals

Awesome

sir.. can we use colony pcr to check transformed bacterias?

How much cost of restriction enzymes and marker? Sir

Hi sir Your video is very helpful Please provide western blotting technique also sir

Sir frm which institute are u working....I have to do southern blotting of my samples....can you please provide ur contact number

Very helpful video

sir can we this plasmid dna as an standard in real time pcr?

I meet to you prosibal?

Excellent Sir, thank you for sharing this full procedure.

Thankyou ...it takes a great effort to make a video. Keep up the good work. Understood really welll

Hello sir....I want to know if all the procedures need to be done in sterile condition after the 3 steps you mentioned for viable cells..? how to maintain that condition? Or not needed?

Question 5..ans--c.. thylakoid space or membrane

very help full video for my end sem practical thank you sir you r a "w person💪 "

I don't get gel

so my takeaway from this is ... Gene cloning involves the use of a cloning vector, such as pbr322, and the gene of interest. Both the vector and the gene are cut using restriction enzymes, such as EcoRI, leaving flanking ends for ligation. The ligation is performed in the presence of ligase at 16°C overnight. The host for the gene cloning is E. coli, specifically the DH5 alpha strain. On the first day, the host is revived and inoculated in Luria broth for overnight incubation at 37°C. The following day, competent cells are prepared and transformed using the recombinant DNA from the ligation. After transformation, the colonies are selected based on their fluorescence under UV light. Non-fluorescent colonies are recombinant, while glowing colonies are non-recombinant and have intact GFP genes. This is divided into three days: the first day for reviving the host and setting up the ligation, the second day for preparing competent cells and transformation, and the third day for colony selection. Bullet Points: - Gene cloning involves a cloning vector and a gene of interest - Both are cut using restriction enzymes and ligated in the presence of ligase - E. coli DH5 alpha is used as the host - The practical is divided into three days: host revival and ligation, competent cell preparation and transformation, and colony selection - Non-fluorescent colonies are recombinant, while glowing colonies are non-recombinant and have intact GFP genes. So far so good?

All tubes which u put in PCR machine having same content?

nice video..... you have summariezed my 6 hours practical class in just 25 min ,but you should explain a bit more with the banding pattern that how the two REs are cutting the DNA..

Sir, can you pls share your mail id