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@MrMyfra 6 ай бұрын
Yes, BUT How did you get pDNA from mRNA? was it by reverse transcription, followed by cloning the cDNA into a plasmid vector, such as pmRNAxp? and then use the plasmid DNA as templete for in vitro transcription? these are not clear from this talk!?
@MrMyfra 6 ай бұрын
How did you get pDNA from mRNA? was it by reverse transcription, followed by cloning the cDNA into a plasmid vector, such as pmRNAxp? and then use the plasmid DNA as templete for in vitro transcription? these are not clear from this talk!?
@SartoriusBIASeparations 6 ай бұрын
Hi and thank you for your question. As shown on the slide, plasmid DNA is isolated from E. coli, then linearized using a restriction enzyme. Linearized plasmid DNA is used as a template to produce mRNA in IVT reaction. mRNA is then purified from IVT as described in the webinar. Plasmid engineering (cloning) is not a topic described in this webinar. In case of additional questions please contact us directly at: [email protected]
@ulduzulduz8495 11 ай бұрын
Oh sisters tell Parkinson a remedy too🙏
@seaneslami1290 Жыл бұрын
Good content is presented with minimum presentation skills!
@ahmedreyad4666 Жыл бұрын
Kindly Dr can you please send me the ppt of this webinar
@SartoriusBIASeparations Жыл бұрын
Thank you for your comment. Please write to [email protected], so we can send you a ppt.
@christophers4551 Жыл бұрын
😱 𝚙𝚛𝚘𝚖𝚘𝚜𝚖
@giuseppefinore9021 2 жыл бұрын
Jk60
@dejangmajner9645 2 жыл бұрын
Nice video for an excellent product! Making HPLC as PAT available for a routine use will have a profound effect on the industry. Keep up the great work!
@kpdub189 4 жыл бұрын
I want to see the slides!
@SartoriusBIASeparations 4 жыл бұрын
Dear Keith, you can access them here: www.biaseparations.com/en/library/seminars-webinars/1049/exosomes-new-tools-for-industrial-purification-and-process-monitoring