Very useful video. Can you tell us what software you used to analyze the data? There has been no answer to the previous question. Thanks for your attention.
@N3omega2 ай бұрын
Good I don’t have to wonder if I’m getting hiv or drugged never know whats in your goodie bag
@agajugowicz57053 ай бұрын
hello :) is it possible to damage the antibodies by vortexing them after dilution ?
@yueunmin47557 ай бұрын
how long can i storage the antibody-coated elisa plate in the refrigerator?
@Bio-Techne7 ай бұрын
R&D Systems has validated the DuoSet plate preparation method as written in the product datasheet - plates are coated overnight and assayed the next day. Longer incubation of the Capture Antibody plate coating (step 1 of the Plate Preparation) is not recommended. For short-term (24 hour storage), stop at the blocking step (Step 3 of the Plate Preparation Protocol) and store the plate wet in the Reagent Diluent blocking buffer overnight at 4 degrees Celsius. Equilibrate the plate to room temperature the next day and proceed with assay protocol. Blocking longer than 24 hours is not recommended. For more FAQs please see our ELISA resource page: www.rndsystems.com/resources/faqs/elisas
@user-tr5bc2gm9h8 ай бұрын
what's the difference between raw material and starting material? Are starting material considered as one kind of raw material, but just more critical?
@itubeutubeyahoo8 ай бұрын
cost?
@Bio-Techne8 ай бұрын
Hello - please fill this request form to receive information on pricing for Jess: www.bio-techne.com/p/instruments/simple-western/request-quote
@santoshyalawar427 Жыл бұрын
Whomuch instrument cost test Manu
@PutiMeister Жыл бұрын
Is it possible to aliquot the reconstituted standard and store it at -80°C? If so, for how long?
@Bio-Techne Жыл бұрын
It is generally not recommended to aliquot and store reconstituted standards. The suitability for long-term storage varies depending on the analyte. Most standards are intended for single-use only to ensure accurate results. It's best to follow the guidelines specific to each analyte. If you have further questions please contact us: www.bio-techne.com/support/contact-us
@user-tj8dx3vq7j Жыл бұрын
Saved my morning, thank you
@petervuong5221 Жыл бұрын
How long does it take to do the entire workflow?
@penetrate111 Жыл бұрын
Hello mam you have provide stem cell therapy Neurogenic bladder
@isal2281 Жыл бұрын
Is it mandatory to use controls with each run?
@Bio-Techne Жыл бұрын
It is not mandatory, but we recommend testing controls in each assay for evaluating performance. Please refer to your kit booklet for more information or contact our support team: www.bio-techne.com/support/contact-us
@khaledelhusseiny4131 Жыл бұрын
Wonderful talk!!!
@pauljoseph712311 ай бұрын
thank you!!
@arianescajeda639 Жыл бұрын
What is an IP?
@user-ux3bl7ke5y Жыл бұрын
5:40
@manishhari54872 жыл бұрын
Hi. Really nice and useful video. Can you please tell which software you used for data analysis? Thank you.
@FelipeFreitasdeCastro2 жыл бұрын
Great lecture
@InquilineKea2 жыл бұрын
wow, chymotrypsin-like cleavage (beta5) might be most important for longevity 34:37 "key lysine residue" at 27:00?
@bioscienceswithshahtareenswati2 жыл бұрын
very helpful
@timothyvernon27022 жыл бұрын
How does this get into the cell?
@seussdoctor94522 жыл бұрын
Thanks for this great lecture! It includes so many insights, facts and pitfalls that are so easy to overlook.
@pauljoseph712311 ай бұрын
thank you!
@cmontenegro88162 жыл бұрын
Very nice video. Sorry to ask, is it necessary to have the handheld magnetic washer?
@JamMcG2 жыл бұрын
Yes! The magnetics attach the beads to the base of the well so they are not lost when discarding the solutions. Without the magnetic washer the microbeads will go down the sink too and you'll be left with nothing to analyse! Make sure you allow 1 min for the beads to settle before discarding solutions too.
@rhyothemisprinceps16173 жыл бұрын
9:30 start of presentation 13:20 ubiquitin-proteasome pathway summary slide - ATP required 15:22 *antigen presentation* - 1% of product peptides are taken up by ER and displayed in cell surface by MHC class I molecules - proteasome allows immune system to screen intracellular space for abnormal proteins (pathogen or cancer) 23:16 multiple ATP dependent steps in proteasome function 38:00 Bortezmib - proteasome inhibitor, inhibits NF-kB, does not cross BBB, induces apoptosis 40:33 Endoplasmic-reticulum-associated protein degradation () pathway is unuslaully activated in multiple myeloma cells ; proteasome inhibition results in increased ER stress and triggers UPR, further UPR activation triggers apoptosis 42:30 proteasome inhibitors are not more toxic since they mainly block chymotrypsin-like site & only partially inhibit protein degradation ; since myeloma cells have to break down lots of immunoglobins they become more stressed than other cells as a result 43:27 cells can compensate for proteasome inhibition thru Nrf1 activation (more proteasome) and increase in autophagy / lysosomal degradation 44:35 cAMP phosphorylation of 26S proteasome enhances degradation of misfolded proteins 45:50 cAMP dependent regulation of protein phosphorylation ; rolipram is PDE4 inhibitor 47:39 cAMP activation for treatment of neurodegenerative diseases 49:06 rolipram for taupathology ; rolipram increased proteasome activity and reduced tau accumulation in mouse model 50:36 summary slide - endogenous regulation (ubiquitination, DUBs) ; exogenous activation - phosphorylation of proteasome 52:13 Q&A
@nageswarraorao17543 жыл бұрын
very good video
@carmengarrimurcia3 жыл бұрын
Thank u !! I have an exam tomorrow of this and It helped me a lot
@MrBepis-qx5ye3 жыл бұрын
What is the purpose of phosphate buffered saline?
@user-sm5ww2ne9x3 жыл бұрын
it mimics physiologic pH and ion concentration
@mamostamuhamad73263 жыл бұрын
thanks for this video
@denisepaulsenful4 жыл бұрын
But what isn't addressed here and most commonly anywhere else is how long do we need to fast and at whicj juncture - is it at 16 hrs without food or 24 or 36? Then how long do we stay without food for this to be optimally beneficial. Tell.us the whole story. Break it down to a simplistic practical application please.
@gigigarcia36913 жыл бұрын
I’ve seen in other studies that a3 day fast is optimal
@ChipChapinSJ4 жыл бұрын
On your investor web page you titled this video "Epic Tools for Epic Science" (investors.bio-techne.com/). That is a lot more compelling than "A Reputation of Quality: The Bio-Techne Brand Story". Own it.
@s.sankaranarayanan5404 жыл бұрын
Hey. Excellent webinar!!. I have one question, in normoxia, how many passages can we culture the MSCs?.. Also, we are facing difficulty in trypsinization of MSCs. any suggestions??
@potato74754 жыл бұрын
TrypLE
@Bio-Techne4 жыл бұрын
Hi S.Sankara, sorry for the delay in our reply. Please see the response from Marnelle Anderson, one of our research associates. "If you are growing cells in high FBS, then sometimes you may need to rinse several times with PBS, or perhaps to quickly rinse with trypsin prior to trypsinization. If you are growing them in serum free media, they should come off easily. The caveat is that MSCs get stickier when they start to differentiate. As to how many passages you can expect out of primary MSCs, this is trickier, since experience has shown it depends on the quality of the MSC, where they are isolated from, and the media. In general, I would say 5-10 passages… five being a decent/ average number and 10 being a great one. " Please let us know if we can answer any additional questions.
@uniqued4ve4 жыл бұрын
I love your slides. Very comprehensable explanations too 5|5
@Drakecj9935 жыл бұрын
Does this work with all cells lines?
@gguniiit29535 жыл бұрын
Nice video
@PUCOPZ5 жыл бұрын
Good work!
@edthoreum76255 жыл бұрын
23:00 ,2nd lecturE obecity impact on stomach & cancer
@indiaindia56055 жыл бұрын
Is it in the competition with Organs on Chip technology ...this is more accurate and has more reliable results ...
@pkleong85 жыл бұрын
I am not from the scientific community. However I have been practicing “ starvation” to activate autophagy the last 10 years without realizing it. I take just 1 meal daily. Dinner. I skip breakfast and lunch. I do not eat anything after dinner. No special diet. What is most interesting to me is the realization that the ancients have knew about autophagy all this while. Muslims fast. The gurus of India and the lama in Tibet and Nepal fast on a regular basis. They know the benefits of autophagy. The only difference is the do not understand mechanism of it until 20th/21st century with the ground breaking discovery of Dr Yoshinori Ohsumi. It is also very interesting to find out that certain existing drugs may help in activating autophagy. Metformin for example has is commonly prescribed for diabetes.
@vister67574 жыл бұрын
May be the reason why when we r not felling well we don't have the appetite to eat and just sleep n rest until we r well may be part of how our bodies heal themselves? Hmmm...
@dryfastingclub5 ай бұрын
look up dry fasting for the true fast ;)@@vister6757
@turkmusik5 жыл бұрын
Go to 3:41 to skip the tedious introduction
@gerarddeclerck39394 жыл бұрын
You can say that again... damn
@ldjt61846 жыл бұрын
So people with ms should avoid dairy?
@catherineneil51916 жыл бұрын
thank you for uploading this, I am beginning my honours project this year which is related to ARMeD destruction of endogenous proteins. this video on SUMO has helped me get my head around the basics.