Can I lyse the cells directly from the vessel? And after this protocol, is it necessary to do a RNA cleanup or I can skip that?
@AMINTAHERI-q5v13 күн бұрын
Hi I would like to thank you for the amazing video. Do you mind if you could tell me based on which book you are making this tutorial? Another thing is that how can I find the graph that you used while lecturing?
@JYOtiRaNJanMANgaRaj6 ай бұрын
Thank you so much
@JYOtiRaNJanMANgaRaj6 ай бұрын
Thank you so much 😊
@BryceGallie9 ай бұрын
Inspiring, life changing, Tara says love the hair
@dhvijpatel91389 ай бұрын
Mono chlorobenzen
@rajukodala2059 Жыл бұрын
Pl. Video on spray drying and fluidized bed drying time and heat and air required
@blackbean664 Жыл бұрын
The cells must not be left without medium or Trypsin solution for 5 mins
@harekrishnakumar2803 Жыл бұрын
Pls increase the video quality(720p) so that it could be more visible to us... By the way, excellent explanation..
@prescaray Жыл бұрын
Hi how can i get the google drive link?
@tulsijoshi1046 Жыл бұрын
If she discarded the media won't the cells go with the media?
@eversincekorea Жыл бұрын
the cells are adhered to the flask
@funny117445 ай бұрын
Only the dead cells go with media, the adherent cells are fine. Which is good for the healthy of last one. I think.
@Carl-Ernst-Otto-Kunze Жыл бұрын
@Zauberberg
@andrea5829 Жыл бұрын
And how without autosample?
@moonlikeyou3339 Жыл бұрын
How long do you change the medium?
@asifaanwar5696 Жыл бұрын
Hi. Can i know how to unscrew the cap if it's very tight after we perform the experiment?
@samuelarnold5792 жыл бұрын
This has been approved by the fine folks of Team Corruption
@alialqallaf41272 жыл бұрын
I am extracting RNA for RNA seq from mice skeletal muscle. How much in weight should I use to make my lysate with what volume of RTL?
@divyaagrawal67402 жыл бұрын
video quality is too bad
@thanyarathnueangchamnong40922 жыл бұрын
Hi sir when i'm using the same condition(Reflux ratio) sensitivity didn't get the error but in design spec getting error ERROR RADFRAC NOT CONVERGED IN 200 OUTSIDE LOOP ITERATIONS. what should i do sir
@bjornvanderlande74912 жыл бұрын
Still an perfect video to do this in 2022!
@Suprbhat2 жыл бұрын
So, for each different starting composition we need to do the same and then will get whole graph and curve ?
@juancarlosquintero96945 ай бұрын
yes!!
@fatemehrajaee5332 жыл бұрын
Hello, when I run my file, the mass flow and mass fractions do not appear in the stream result? what I should do?
@xxkkb86852 жыл бұрын
look at that furry bracelet on the right wrist :)
@osmirod12 жыл бұрын
The t75 must remain with the cap on at all times except when pipetting
@alejandrogonzalezalmazan34322 жыл бұрын
Does it really mix the PCR reagents?
@aymanedaiz67662 жыл бұрын
thank you so much from Morocco for the vidéo. I have just a question do you think 5 minutes it's too long, maybe 1-2 min sholud be enough.
@ariajohn372 жыл бұрын
Depends on the cell line.
@aymanedaiz67662 жыл бұрын
@@ariajohn37 Thanks Aria ✌
@kevinbaboolal18592 жыл бұрын
Great video but for the liquid and vapor phase data at the start of the video ..that's the inlet streams right?
@everythingishere9932 жыл бұрын
Can you give me your contact No i wana discuss something about this machine thanks allot
@richelleparker33392 жыл бұрын
Thanks dude 👍.
@davvader2 жыл бұрын
Nooo, don´t spray into the LAF, this hurts the filters.
@davvader2 жыл бұрын
And the caps of the flask with the open side up!
@12823matthewkao3 жыл бұрын
you didn't wash the culture with PBS before adding the fresh media?
@taniarodezno21663 жыл бұрын
This was so helpful thank you!
@samratpaul253 жыл бұрын
Are any animals killed during the extraction of Trypsin from their pancreas ? Or can it be done without killing the animal ?
@infinitefuture18283 жыл бұрын
You shouldn't wear socks while performing experiments
@funny117445 ай бұрын
it depends on how skilled you are and it depends on the cells.
@kietjaspers76993 жыл бұрын
what happens when two different powder materials get mixed in this proces?
@aishamohammad79783 жыл бұрын
Starting RNA work for the first time so this was very helpful, thank you!
@noura79893 жыл бұрын
If I say loading a plate instead of pipetting a plate, is it correct?
@thenewchicken13393 жыл бұрын
Wow thanks! this helped for my project.
@madisondrum91794 жыл бұрын
She used high speed both times lmao
@cristophercastillo93214 жыл бұрын
Thank you so much!
@sueherschlag44884 жыл бұрын
You make your Momma Sue so very proud!
@themoonmesk65274 жыл бұрын
Great explanation! very helpfull!!
@enkefamily4 жыл бұрын
This is a great resource, thanks!
@funny117444 жыл бұрын
Please mention what type of cells are ? Are human cell ?
@govindsoni37824 жыл бұрын
could u plz make the same vedio using Excel option....
@3dprintwiz3784 жыл бұрын
How about an aircooled radiator. What is it? Is it counterflow or cocurrent flow?
@txnightsky4 жыл бұрын
Neither, it has an "infinite" amount of air to cool it so the Tc in and out stays consistent, so both formulas should give the same answer...
@vernelljohnson69214 жыл бұрын
HI MY NAME IS VERNELL DWAYNE JOHNSON I AM THE INVENTOR OF THE NIGHT GLO SAFETY TIRES U.S. PATENT.I NEED YOUR HELP PLEASE CALL ME @ 909-746-1168.PATENT # 6,709,138.PLEASE CALL OR EMAIL ME. THANK YOU.
@screwlikerabbits4 жыл бұрын
women in stem rise up
@PLF...2 жыл бұрын
They are at home pregnant though
@wadewen10084 жыл бұрын
Could you explain in detail about why you entered the -1.3 for the reflux ratio?