Interesting to see the differences between cgmp vs school.

What is the composition of the liquid used for discharge cells that are not used anymore in the process ?

please don't overcrowd the workspace, cap facing downward, gloves with long sleeves, ethanol spraying your hands with gloves, when moving in and out of the chamber, not speaking while doing work or wear a mask for added protection, have the discard station separate from the other stuff, and clean it well with ethanol and turn on the UV light after completion of use. That's all I remember.

thank you!!!

so many bad practices here. so many things enter the BSC without being sprayed with EtOH, splashing detergent that eats cell walls. exposed skin, blocked air flow, sticking the micropipette directly into a source container. basically just do the exact opposite of what this lady did and you will be fine

isn't it safer to aliquot portions of media and such in 50 ml tubes, instead of sticking the whole micropippete into the liter bottle? that's the way I do it.

4:18 "I multiply by 10 to the 4, meaning I'm converting my uL to mL". Wait, I'm confused. There are 1000uL in 1mL, so to get from uL to mL you would multiply by 1000, which is 10 to the 3, not 10 to the 4.

2 obvious mistakes in this video. 1. overcrowded workspace that is blocking airflow through the cabinet and especially the tubes of media would have no airflow protecting them where they are. 2. there is a gap between gown and glove which leaves open to higher risk of contamination. gloves should always go over the cuff.

Blocking the laminar flow intake vents...this is a class II biosaftey cabinet. It protects the cells from contamination not the user

Yeah I dont know why I hate her. Reminds me of my former job Colleagues who I'm secretly punching in my daydreams.

The thing is that if your using sterile gloves there shouldn't be a contamination problem from the hands. Why she's not using sterile gloves to begin with is beyond me, as it would seem like sterile gloving using a closed-glove technique would avoid about 75 percent of the potential problems.

The amount of overcrowding in the biosafety cabinet shown here will have a serious impact on its effectiveness both in keeping a sterile environment and protecting the user (if using infectious materials). Really poor practice!

What do you think of disinfecting gloves instead of regloving after touching contaminated surfaces?

funny to make a video about cell culture mistake when at the first place they make a mistake by having an overcrowded hood.

thanks you , but I from VietNam , I know a litte English ,Can you write protoco of Imunocytochemistry in here?

the cap should be downward, media can be kept at centralback, too much of equipment and reagents not sure if all are really needed. Then the discard bin should be outside (except whatt I saw is a sterile pippette).

It's a very overcrowded workspace!

thx for the video，but should we encase the sleeves in the gloves，for the watch was not sterile

Thanks lucy, you are so good teacher.

Really great video except for the initial mixing of cells. That's not effective. In order to attain an even distribution of cells in that suspension, it would be essential to mix using a P1000 at the very least. This is important because otherwise your cell count is gonna be way off.

gut gemacht...

Thank you this video is very helpful and detailed, it will definitely help us do the cell culture in lab. - Anni Eloyan

Very detailed & helpful. -Janet Rosas

I feel ready and excited to perform this procedure in class! Thank you for the detailed run down of the procedure. -Alysha Baker

i will definitely try and not make those mistakes! Jason Assanah

thanks for the visual instruction, it really helped!! Jason Assanah

Seems very easy to forget to keep the center area clear. It's going to take some practice.

Thanks for making this! I'll feel pretty accomplished if I can manage to do this without a bunch of mistakes.

why can't all bio classes post tutorials like this :]

Thanks for doing this video! It outlines the steps very clearly and is very helpful.

Going to have to take special care to avoid these...

Great video! It was very discriptive and outlined the procedures very well.

There are a ton of steps, however the video did a great job explaining each step in detail; as well as explained why the steps were being done. I really enjoyed it and I hope I can remember all this for tomorrows lab.

nice figure real talk

good editing to illustrate all the steps

The person behinds the scenes is so inquisitive.

These tips are amazing, hopefully I can create a pure cell culture and avoid any errors, even though the cell culture seems like a lot of work it seems fun. Excited :)

There are so many steps I think I might have to watch it two more times and write the steps down because this is a lot to remember. Thank goodness someone is able to show us how to do this before hand that way the source of error is reduced.

I liked how the video was very descriptive and is really helpful hopefully it will be as easy as it seems doubt it but looks like a lot of fun.

This is gonna take some practice... -Sam

I hope I'm not that guy who makes all these mistakes... -Sam

All this technique to remember is giving me a headache! ahhhh! -Sam

So is the PBS just a cleaning process for the cells? When doing this, how do you know you are not extracting the cells while taking the substance out?

Its Estefania again. Why do we have to pipet the medium out from the corner? wont cells get sucked up?

-Estefania Gomez. Whoooo! im the first one :)

-Estefania was here :) this looks cool! Im excited to try it out

Great job!!

Great job Pablo!!