You're so welcome! Glad it was helpful.

You are very welcome!

We recommend methanol fixation because it typically results in a clearer stain. Over-heating of organism on a slide can sometimes cause it to deform or change shape due to heat exposure. Methanol fixed gram positive organisms are also less likely to de-colorize during the staining process.

@@HardyDx will try tomorrow 🌟 thank you

@JesusLightsYourPath Great question. The reason that the metal loop is sterilized for each quadrant is to eliminate extraneous 'crossover' of organism when you streak each quadrant out. By the final streak, the number of cells on the loop should be diluted and low enough that single colonies are dispersed and isolated on the agar surface. If you were to not sterilize between streaks you would end up with a much more confluent 'lawn' of bacteria because of all the extra organism on the loop when you go to streak your successive quadrants. To learn more about how to properly streak a Petri plate visit our blog. hardydiagnostics.com/blog/petri-plate-2023

Hi Aakif Thank you for your message. To view any of the job openings we have available at Hardy Diagnostics, please visit our website link below and apply there: hardydiagnostics.com/job-openings

Hi @josephmaloney-jn1bg we appreciate your question and positive feedback. At Hardy Diagnostics we strive to provide a Culture of Service. To help answer your question I have included a section from our instructions for use (IFU) for the Stat Stain aka One Step Wrights Stain below. I believe this will address your needs. For the full IFU for One Step Wrights Stain (Cat. No. SS016) visit the product page on our website and look under the supporting documents tab. hardydiagnostics.com/ss016 Here are the instructions for the One Step Wrights Stain: STAINING PROCEDURE FOR BLOOD SMEARS AND BONE MARROW: 1. Prepare a film of blood or bone marrow on a microscope slide and allow to air dry. 2. Prepare three containers (e.g. coplin jars, or staining dishes). Fill one container with One Step Wrights Stain and the second and third containers with distilled or deionized water. 3. When stain volume in container 1 becomes insufficient, replace the stain. Do not replenish by adding new stain to the old. 4. To prevent evaporation, keep stain tightly covered when not in use. 5. Change the water in container 2 or 3 when an iridescent scum forms on the surface or when a dark blue discoloration occurs. It is very important to keep the rinse water clean. 6. Dip air dried slides in One Step Wrights Stain 15-30 seconds. 7. Dip slide in distilled or deionized water in container 2 for 15-45 seconds. 8. Dip slide in distilled or deionized water in container 3 for 25 seconds, using quick dips. As an alternative, the slide may be "swished" in distilled or deionized water for 25 seconds to remove the stain. 9. Wipe the back of slide. 10. Dry slides in vertical position, on absorbent surface. Do not blot the smear. 11. Apply oil and examine microscopically. * For bone marrow smears, double all the above times. Additionally, if you would like to learn more about the Wright-Giemsa Stain we have prepared a blog that can assists you. hardydiagnostics.com/blog/how-to-do-a-wright-giemsa-stain

Hi @josephmaloney-jn1bg we appreciate your question and positive feedback. At Hardy Diagnostics we strive to provide a Culture of Service. To help answer your question I have included a section from our instructions for use (IFU) for the Stat Stain aka One Step Wrights Stain below. I believe this will address your needs. For the full IFU for One Step Wrights Stain (Cat. No. SS016) visit the product page on our website and look under the supporting documents tab. hardydiagnostics.com/ss016 Here are the instructions for the One Step Wrights Stain: STAINING PROCEDURE FOR BLOOD SMEARS AND BONE MARROW: 1. Prepare a film of blood or bone marrow on a microscope slide and allow to air dry. 2. Prepare three containers (e.g. coplin jars, or staining dishes). Fill one container with One Step Wrights Stain and the second and third containers with distilled or deionized water.

3. When stain volume in container 1 becomes insufficient, replace the stain. Do not replenish by adding new stain to the old. 4. To prevent evaporation, keep stain tightly covered when not in use. 5. Change the water in container 2 or 3 when an iridescent scum forms on the surface or when a dark blue discoloration occurs. It is very important to keep the rinse water clean. 6. Dip air dried slides in One Step Wrights Stain 15-30 seconds. 7. Dip slide in distilled or deionized water in container 2 for 15-45 seconds. 8. Dip slide in distilled or deionized water in container 3 for 25 seconds, using quick dips. As an alternative, the slide may be "swished" in distilled or deionized water for 25 seconds to remove the stain. 9. Wipe the back of slide. 10. Dry slides in vertical position, on absorbent surface. Do not blot the smear. 11. Apply oil and examine microscopically. * For bone marrow smears, double all the above times.

Additionally, if you would like to learn more about the Wright-Giemsa Stain we have prepared a blog that can assists you. hardydiagnostics.com/blog/how-to-do-a-wright-giemsa-stain

Thank you Dan. I am glad we were able to illustrate the lateral flow assay process in a clear and precise way.

Sir I need job I'm lab technician I'm from India

@brystopczynski1295 Thank you for your comment. Yes, this is the correct technique used by microbiologist. When streaking a plate the goal is to reduce the number of cells to isolated colonies from quadrant to quadrant. So when the narrator says overlap no more than 2-3 times, it means only overlap from quadrant #1 to quadrant #2, 2-3 times, then continue to streak the remainder of the quadrant. Each time you go to a new quadrant only overlap your streak 2-3 times, then finish out the streak with the 10 -20 passes shown in the video. Your feedback is heard, and we will continue to improve our videos. We are committed to providing a Culture of Service. If you have any other further questions don't hesitate to reach out. Additional if you would like to know more about the four quadrant streak feel free to visit our blog to learn more about the process. hardydiagnostics.com/blog/petri-plate-2023

You are welcome @genesatina3000, we appreciate you too!

@vanillaaffogato9985 We recommend methanol because it typically results in a clearer stain. Over-heating of organism on a slide can sometimes cause it to deform or change shape due to heat exposure. Methanol fixed gram positive organisms are also less likely to de-colorize during the staining process.

@@HardyDx wow thank u very much. Very kind of u

Glad you liked it and thank you for the feedback!

Aerobic organisms must be taken from an 18 to 24 hour old culture. Organisms lose their catalase activity with age. For slower growing anaerobic organisms, an older (24-72 hours) culture may be acceptable.

Glad it was helpful!

Thanks, will do.

Glad it helped!

You're welcome!

No.

Yes , lots of opportunities

Yes u will get job but better u can complete MSc then u will get better opportunity

Not immediately, get jobs that get you expirience in USP testing that will raise your chances

Thank you for taking the time to watch our video and for sharing your feedback with us. We truly appreciate your input, and we want you to know that your suggestions will definitely be considered in our future video planning. Regarding the background music volume, we understand your concern. Unfortunately, we are unable to make adjustments to previously posted videos without affecting our current viewership and traffic. However, please know that we are continuously striving to improve the quality of our content based on valuable feedback like yours. Once again, thank you for being a loyal viewer, and we hope you continue to find our videos informative and engaging.

Colonies will still grow, yes. However, it is usually best to restart on a fresh plate as the puncture could lead to difficulties in reading/counting colonies or could cause unwanted areas of heavy growth. Don't worry though! Marring the surface is extremely common, especially in beginners. Keep practicing and you'll be able to do it as if it were nothing!

Quit yapping.