Veterinary nursing student here 🙋🏻‍♀️ we are covering histology and cytology this week thank u for this helpful information 🙏

If you ask them to Google everything what was your role here. Waste of time!

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Excellent talk. I highly appreciate the good work. Currently working on rnaseq work.

Maravilhoso! Muito obrigada

Hi,Thank you so much Candice! This is a very well structured lecture and I absolutely loved it, I am beginner and I need more help to understand RN sequence analysis.

Thank you for a great video! But i just can´t seem to see the answers to the quiz... Could anyone who can see them help out? Thanks!

Terrific information for RNA-Seq data analysis.

A TRANSCRIPT WOULD BE SO HELPFUL, BECAUSE THERE ARE PORTION/WORDS THAT ARE MUMBLED. PLEASE

Thank you, very helpful video!

How can we understand that we have less than 12 replicates or not?

Definitely using this my literature review thanks

Awesome work!! Thanks for all the info

hi can someone plz explain which file are we using as index file here at 30:03? and what is an index file.

This was very well done. Thank you, Candice

Awesome talk for the new player in RNA seq! I am just wondering why KZbin recommends your channel to me later.

This was really great to help me understand the entire process. I really enjoyed it. Is there a way for me to do this without having access to these packages like hisat2 and HTseq? I don't think I can download them on my computer and even if I did the flies that will be analyzed by this are too big to download. what are my option if I want to do an independent project?

Can you please give us the answers to the quizzes??

41:41 Anyone know how to obtain the hgnc_symbol? I used cuffdiff which gave me XLOC gene IDs but I can't seem to find a way to effeciciently convert them to common gene names.

Even after years, i found this video is the best for explaining RNA-Seq analysis from top-bottom. Thanks a lot @Candice Chu.

Thanks a lot for this nice tutorial. Do you have any suggestions for the fungal RNA Seq analysis or I can still follow the protocol mentioned in the tutorial.

What a great video! Aside from the well presented information, your tribute to your cat touches my inner emotions. As a soon to retire internal medicine physician, I am progressing in learning RNA-Seq technology in order to pursue my specific research interests. I appreciate your insights and applaud your kindness to learners and all creatures. Mark McCaulley, .MD, FACP

This is one of the best tutorials I've seen on any subject, thank you so much for this!

看到一半突然看到繁體中文的google介面才驚覺你是台灣人! 謝謝您的講解 祝您在美國一切順利! 希望有天也能到達您的高度~

Thank you a lot!

Thank you for the ellaborate explanation and resources. Most interesting talk I have heard about this topic (RNA-seq)

Thank you very much for all the details. And I like your lecture very much.

The best video on RNA seq analysis, also with loads of resources. Thank you.

What is answer of bonus question?

Thank you so much for this video! It opened my mind to know how to analyze my rna-seq data. It was really perfect! Congrats.

Super useful! You know exactly what a beginner needs to know. And that's really interesting you introduce the websites. It keeps us updated. Thanks

Candice, thanks for the video, do you mind sharing the "Presentation.R" script in your video (around 38:35 min)?

Thank you for slow and nice presentation

Thank you for this informative session. It was very helpful for me. I wanted to ask if you have resources available, can I get all.deseq2.R script. I am doing some work with plants around 30 samples.

Dear Candice! Thank you so much and may I ask if I can have the complete R script on your vedio. Some of them has been out of the screen.

My answer is 1. E, 2. C, 3. A, 4. B, 5. A, 6. B, 7. E, 8. D, 9. B, Bonus. B(maybe...haha). I think the last answer is "neoplastic" because the cytoplasm is bluish and there's some anisocytosis, anisokaryosis. Although there are many neutrophils I think it's a kind of secondary change. Please let me know the answer whenever you want.

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Thank you, I learned a lot

This presentation was awesome! My PPT game just improved 100%.

Hey Candice I’m wondering if your answer to the question about why RNA-seq is used instead of Protein profiling, you mentioned the current technology isn’t sensitive enough to profile all the proteins. But I think RNA- seq has the same problem. Are you able to sequence all the RNA molecules in present in that condition?

Hi, can you please prepare a video on artifacts in blood smear?

Follow my Instagram ➭ instagram.com/vet.clin.path.resident/ Follow my Facebook ➭ facebook.com/Vet.Clin.Path.Resident/ Follow my Twitter ➭ twitter.com/vetclinpath My flowpage ➭ www.flowcode.com/page/vet.clin.path.resident

Thank you so much for your work!

Which file were used for index building using HISAT2-build?

Not only Gene's phenotype depends on many parameters!! This is wrong

We need to study a specific cells expression , protein is an end point ..so why the question??

Could you please tell me what your controls were in your experimental design?

Hi Candice, firstly, thank you for your video. I'm watching in 2021, but maybe like you I'm stuck in 2016 as my dog and best friend passed away about a month and a half before your cat (1996.01.09-2016.08.14), so I sympathize deeply. Life will never be the same, but I'm trying to throw myself into research to see what I can learn about what it all means...

Very interesting lecture:)