Thank you, greatly appreciated! :)

Sorry....I don't know what you mean

Looks like this is an exam question... is it? :)

@@dobergeron yes. and i jumped it. Full quesion: Standard addition is an analytical technique of extreme importance to an analyst, as it eliminates the interfering effect in most determinations. Fluoxetine is widely used as fluoxetine hydrochloride, also known as Prozac®. This is indicated in the treatment of depression, associated or not with anxiety. This compound absorbs ultraviolet light at a wavelength of 227 nm. It has in its structure a stereocenter, being a chiral drug. It is known that only the (R) -fluoxetine isomer has activity, while the other enantiomer has no pharmacological action and there is no evidence of toxicity to the organism. In an industry, when performing the Fluoxetine analysis, the following analytical calibration curve was obtained, in which the concentration is in g / 100 mL: Abs = 372 CFluoxetine + 0.6432. Knowing the analysis was performed according to the standard addition technique and that the molar mass of fluoxetine hydrochloride (C17H18F3NO.HCl) is 345.79 g / mol. Determine the mol / L concentration in the analyzed solution.

​@@dobergeron I thinks i need to use this equations but i dont know how. S=k.Ca y = m*x + b Y = S x = Vs m = k . Cs / Vt b = k.Cx.Vx / Vt It's a friend whom sent me and i couldn't to do it. I researched i found this 3B4 equation. ( www.mathcentre.ac.uk/resources/uploaded/3b4printableversion.pdf ). But i dont know the source from y value which has been used. I have the alternatives but idk which is the correct: 2,25x10-5 mol/L 5,58x10-5 mol/L 5,0x10-5 mol/L 9,69x10-5 mol/L 3,08x10-5 mol/L

As a professor myself, I don't think I should comment on your question. I'm sorry, ask your prof... I'm really sorry but I can't give you the answer. There are a lot of resources available, you can do this. Isolate each variable and look how to solve. Hint: look at extinction coefficient... ;)

does adsorption of chitosan absorb at any wavelength compared to non adsorbed? (I assume the aDsorption word is not a typo, very different from aBsorption...

@@DominicBergeronPhD please guide in this regard...

@@almaanmani7361 you need to be more specific. Sorry, I'm not sure I understand what your question is. Really sorry....

@@DominicBergeronPhD sir, when we plot graph between absorbance and concentration then what's the effect of dilution I mean if absorbent is dilute then more absorbenc yes or not ? nd how will we form a standard solution....?

@@almaanmani7361 a .more diluted solution will give you lower absorbance values, that's Beer's law. I'm not sure with chitosan, I never worked with it, but in order to make a calibration curve you will need to make several solutions of chitosan and read the corresponding absorbance at a specific wavelength. Make sure you cover a wide range of concentrations and be aware of the limitations of your instrument

you need a substrate or products that absorb uv light

kzbin.info/door/clJKlJplPCcFNKd-HzSwgA

Glad it helped!

Most welcome!

Sir, how we plot graph for adsorption of chitosan..?

what is weighting factor

True! But you can always dilute your sample to make sure it fits within the available data. Knowing the dilution factor will let you calculate the exact value. It's that simple!

If R2 is less than 0.9, it might not apply at all because the relationship might not be linear. It certainly doesn't apply if R2 is 0.25!