Amazing story. Dr. Blaine Roberts and his wife Anne are so inspiring. Loved seeing their kids in the lab too! Very cool use cases for spatial proteomics. Would love to see more documentaries like this from Bruker!
@ianloke72284 ай бұрын
Great talk Nicki!
@marlentoktomamatov58685 ай бұрын
this looks supercool🔥
@alexandramccormick70637 ай бұрын
This Is The Greatest Workplace Demonstration Of All Time
@xheni70111 ай бұрын
My Name Is Maldi😭😭😭😭😭😭😭
@jordankanelai4326 Жыл бұрын
im too dumb to understamd this
@HusainMithaiwala-b4j Жыл бұрын
Great presentation! Could you shed some more information on how the PET samples were prepared for MALDI? Solvents and the matrix (and its solvent). I am utilizing a Bruker Rapiflex MALDI-TOF and was not able to get any peaks on PET bottles.
@nikhilgoyal7416 Жыл бұрын
we want to analyze a mixture of polymers (very low molecular weight resins like hydrocarbon resins & high molecular weight resins like EVA) on a bruker equipment. Please inform what matrices should be used?
@prestonwing Жыл бұрын
Hi and thanks for the video. I am quite curious about the application pros and cons between QTOF and TOF/TOF. I understood their difference in terms of mechanism (Quadrupole as mass filter vs ToF + timed ion gate as mass filter) but I am having a hard time finding which one is "better". Furthermore, I noticed that QTOF are predominantly coupled with ESI while ToF/ToF almost exclusively with MALDI. Is there a reason behind this?
@blacklag342210 ай бұрын
I am not a Bruker employee but I work for my PhD in the field of mass spectrometry, so let me try to answer your questions. qToFs are typically coupled with ESI (or other API sources) because the ToF measurement requires a defined starting point. In case of MALDI, this starting point is defined by each laser pulse and, hence, MALDI is directly coupled with ToF analyzers. In case of ESI, the starting point is defined by an orthogonal accelerator (oaToF) that accelerates the ions orthogonal to their original flight path. This oaToF set-up allows for coupling with quadrupoles as the orthogonal accelerator requires low ion speeds, which are also required for quadrupoles. The MALDI ToF coupling, on the other hand, provides extremely fast moving ions which prevents the usage of quadrupoles in these devices. Answering the question about dis/advantages of qToF vs ToF/ToF is hard because both techniques are well suited for acquiring MS/MS spectra. Overall, its rather a question of which ionization technique is more suitable for your samples. However, I personally prefer the qToF set-up for MS/MS experiments because quadrupole collision cells allow for measurements at well defined collision energies which are really important for my research.
@aaaaa-yv1zr Жыл бұрын
low efficiency
@yogamon Жыл бұрын
Impressive data, they cost about $150k?
@abhaypandey9822 Жыл бұрын
What is the LOD and LOQ for any method in this instrument
@BrukerDaltonics Жыл бұрын
Dear Abhay Pandey, the LODs and LOQs are highly dependent on the analytes selected, matrix analyzed and method used. For example, you can achieve sub-ppb LODs for most of the pesticides in fruits and vegetables matrices using QuEChERS sample preparation or sub-ppt LODs and LOQ for PFAS in water samples using direct injection.
@abhaypandey9822 Жыл бұрын
Sir, as I am working on developing method for Quantitation of antibiotic residues in milk and i have bruker lc tqd. I wanted to know that what can be the LOD for methods. Can I go till ppt level with this instrument (lc ms evoq elite ER. Thanking you sir
@BrukerDaltonics Жыл бұрын
@@abhaypandey9822 thank you for being so interested in our products :) We would love to start a deeper discussion with you. Would you mind getting in contact with [email protected] or [email protected]
@Tk-ud2dk Жыл бұрын
Great video thanks
@Deltamass Жыл бұрын
amazing video and great explanation. but the background music is interfering and bit irritating.
@Fon09fon Жыл бұрын
You find it irritating because you are not German resident. After one week in Berlin I think you will like the background music.
@ghada3883 Жыл бұрын
Very helpful!
@dressurkeks4917 Жыл бұрын
Thanks for this good video!!
@metrotmobil68122 жыл бұрын
Very interesting
@jijumon_as2 жыл бұрын
Interesting, whats the price of this?
@heulboje212 жыл бұрын
Yeah so when I clock on "Finish" nothing happens. Window close but there is no New project in the project Manager...
@BrukerDaltonics2 жыл бұрын
Hi Paul, thank you for reaching out. In this case could you please contact our software support? Just let us know if you need the contact details. Kind regards Bruker
@heulboje212 жыл бұрын
@@BrukerDaltonics thanks, your Support helped me out a lot! Is there a part 3 to this series?
@moongihong27942 жыл бұрын
3:47 data acquisition and processing
@JKOOLDK2 жыл бұрын
This is so much higher quality explaination than so many other sources
@armagedonam2 жыл бұрын
Where is part 3?
@mutaherashaikh25412 жыл бұрын
Thnqq so much for making viodi on this topic
@aldomunoz96812 жыл бұрын
cool
@DavidSchilter2 жыл бұрын
Really useful! Thx Chrys, C&EN and Bruker :)
@samirbrahimi57823 жыл бұрын
I wonder how much does it cost !? And for a medical analysis lab is it better to have the maldi 2 or a classic elisa system for hormons analysis !? And how much does it cost only for making it work !?
@BrukerDaltonics3 жыл бұрын
Hi Samir, thank you for your request. Would you like to share your contact details with us? For a detailed answer we would like to hand over your question to one of our product experts. Kind regards, the Bruker Marketing Team
@RohitPant043 жыл бұрын
Excellent animation and sounds!
@Jopau183 жыл бұрын
Damn! The sound effects, the music and narration.. nakaka hyper siya. Haha.
@wesuda3 жыл бұрын
What ionization method is used by the microflex LT mass spectrophotometer?
@BrukerDaltonics3 жыл бұрын
Hi Erwin, thank you for your question. The microflex LT/SH mass spectrometer uses also the MALDI Methode (Matrix Assisted Laser Desorption Ionization).
@wulyx3 жыл бұрын
when will be issued the next video announced at the end of the secon video about data processing in metaboscape?
@DG-pq6vh3 жыл бұрын
👏
@micronerd3 жыл бұрын
Very nice visualisation to revise my understanding of MALDI-TOF. Thanks for this cool upload. I'm currently working on my first-time MALDI application as part of an internship. Very excited about that!
@Aa-ji2yf3 жыл бұрын
Thanks for the explanation
@dollygussie13563 жыл бұрын
gpz vum.fyi
@Stefunny193 жыл бұрын
Hello, I noticed you have another video called "MALDI TOF Process" as opposed to "MALDI TOF TOF Process". What is the difference between the two?
@BrukerDaltonics3 жыл бұрын
Hi Stefunny, thanks for your question. TOF stands for “Time Of Flight”. TOF TOF means “Tandem Time Of Flight”. Both Mass Spectrometry concepts are similar but different during the measurement of your sample. MALDI TOF makes it possible to determine molecular masses while MALDI TOF-TOF provides you an deeper analytical insight of targeted compounds.
@noaholmeijer3 жыл бұрын
This is one of the coolest video's I have ever seen
@ARYANGUPTA-uz1xb3 жыл бұрын
really amazing✌️✌️♥️
@studygroup89593 жыл бұрын
Nice explanation! Can u please tell which software u have used for animation please? Also a lot of love from India🇮🇳🇮🇳
@BrukerDaltonics3 жыл бұрын
Hi Study Group, our Team used mainly "Cinema 4D" for the 3D Animation. For Video editing we used "Adobe Premiere" and "Adobe After Effects".
@studygroup89593 жыл бұрын
@@BrukerDaltonics thanks a lot sir for replying (•‿•)
@chachouromynews3 жыл бұрын
What are the dimensions of the device please ?
@BrukerDaltonics3 жыл бұрын
Hi chachouromynews , in this Video we are showing our "rapifleX". This Instrument has a size of 95 x 80 x 275 cm. Of course we also have smaller Bench Top Instruments, like our Microflex Series.
@fickNutzernamen3 жыл бұрын
whats the name of the track, it's lit af
@BrukerDaltonics3 жыл бұрын
We are glad you liked it, the Song is called "Breaking News" by Goodstreet
@enriquep48573 жыл бұрын
Is it possible to identify specific strain of a species?
@BrukerDaltonics3 жыл бұрын
No, the MALDI Biotyper can identify up to species level. For strain typing we recommend using our IR Biotyper. You can check our product Page for further Information: www.bruker.com/en/applications/microbiology-and-diagnostics/food-beverage-microbiology/ir-biotyper-for-food-microbiology.html
@NuclearCashew3 жыл бұрын
although educative, it's so damn cool as a video at the same time.
@zhang19242Liv3 жыл бұрын
Great talk, difficult to comprehend the principle of PASEF though...
@BrukerDaltonics3 жыл бұрын
Hi Hao Zhang, thank you for your Interest and your feedback. We are explaining the PASEF principle in one of our other Videos: kzbin.info/www/bejne/gGHYeJuvi6mcq7M You can have a look at Minute 01:00 - 01:50, maybe this helps a little bit to visualize the process. :)
@Amarah46003 жыл бұрын
WOW. This is great!
@Amarah46003 жыл бұрын
The background music made this so epic. Thanks for this video. I'm a visual learner and this helped so much