How to Stain an SDS-PAGE gel
5:46
13 жыл бұрын
Gel Extraction Comb Trick
1:36
14 жыл бұрын
How to Run an SDS-PAGE gel
4:44
14 жыл бұрын
How to Use a Centrifuge
3:17
14 жыл бұрын
How to Make an SDS-PAGE gel
4:49
14 жыл бұрын
How to Use a Pipette
5:11
14 жыл бұрын
How to Avoid a Leaky SDS-PAGE gel
2:52
How to Use Parafilm with Agar Plates
1:59
How to Make and pH Buffers
4:01
15 жыл бұрын
How to Pour Agar Plates
2:22
15 жыл бұрын
Пікірлер
@VrittaAmroiniWahyudi
@VrittaAmroiniWahyudi 29 күн бұрын
2024
@cassthesmelly6006
@cassthesmelly6006 Ай бұрын
the background music in this is making me go insane
@SofilaLarasati-v9m
@SofilaLarasati-v9m Ай бұрын
thank you
@SwastiRastogi-n6b
@SwastiRastogi-n6b 2 ай бұрын
For some reason, when I prepare the gel, I notice small webs formed inside the well, which is why when I load the protein, the proteins don't run well. I prepared new apps and tried again, but somehow encountered the same problem, Could you suggest what should I do ?
@_sagarrr_____
@_sagarrr_____ 2 ай бұрын
Saved my life
@LindaTaylor-e7u
@LindaTaylor-e7u 3 ай бұрын
Hall Betty Brown Maria Brown Linda
@VinnithPullavarayar
@VinnithPullavarayar 3 ай бұрын
Thanks was helpful
@teemathit
@teemathit 3 ай бұрын
Thank a lots bro. U save ma life
@sindhowagan9456
@sindhowagan9456 4 ай бұрын
Make video for RNA gell run my bend didnt come when i make gell to see bend
@irvingmarquez1106
@irvingmarquez1106 5 ай бұрын
Thanks so much for sharing
@irvingmarquez1106
@irvingmarquez1106 5 ай бұрын
Thanks so much for sharing!!!
@heathermercer6709
@heathermercer6709 5 ай бұрын
If you have leakage, you can tape the bottom of the plates together after you place the two glass plates in the casting stands and before placing them in the casting frames. This helps ensure you wont have leakage when pouring the lower gel. It is really easy to remove the tape once the lower gel and stacking gel have polymerized.
@ericricci
@ericricci 5 ай бұрын
14 years later and you're still saving lives...just had 3 leaky gel attempts and this video saved my ass
@aoqikehua
@aoqikehua 5 ай бұрын
There is a sealing film cutter that can be used without scissors
@migoihaohang
@migoihaohang 6 ай бұрын
Nice
@MelinaLakatos
@MelinaLakatos 7 ай бұрын
Hi pc pc p0
@philipgoetz8681
@philipgoetz8681 8 ай бұрын
But how can you sterilize the parafilm?
@sivajothi452
@sivajothi452 8 ай бұрын
Very clear video
@ณภัทรคูณขุนทด-ห4ธ
@ณภัทรคูณขุนทด-ห4ธ 10 ай бұрын
My teacher used your video as an instruction video, be proud
@CemreGungor
@CemreGungor 11 ай бұрын
Why you adjust the pH to 8.8?
@bugsy74
@bugsy74 11 ай бұрын
How do you clean the fritted glass after use?
@jadewhite7022
@jadewhite7022 10 ай бұрын
Hi! I am wondering the same thing and was curious if you figured it out- I am wondering how exactly to clean the white part on the base/stopper? Seems like that could lead to contamination unless using the same buffer
@actionspeakz2513
@actionspeakz2513 Жыл бұрын
This process needs practice more and more😮
@فاطمهخواجوئی-ظ6و
@فاطمهخواجوئی-ظ6و Жыл бұрын
You can add a little amount of resolving gel with higher percentage of TEMED to prevent leaky and after the thin layer of the bottom becom solid , you can add standard resolving gel
@shamailarizwan4083
@shamailarizwan4083 Жыл бұрын
Can anyone here educate me how to prepare prf( protein rich fibrin) to use in infected wounds as I m working at periphery n not much facilities r available here , we try to heal n help patients on our own within our resources... I ve got a centrifuge old machine China made 800 centrifuge wth rpm:4000.n rcf:1790×g.capacity 20cc×5. I also wanna know test tube's length its material n time to spin to retrieve max benefit from my produce.though I have general info frm watching diff u tube video but wanna get know frm experts as reading comments here I noticed that quite an experts in centrifuge technique are commenting here. I wd really appreciate if anyone is to extend his knowledge to where it's really needed n be used productively.. Thanks in anticipation n prayers!
@Alan-gi2ku
@Alan-gi2ku Жыл бұрын
Thanks. Just what I was looking for. Don’t hold the Petri dish too tightly, they crack easily.
@gunwol999
@gunwol999 Жыл бұрын
If the filter base stuck, how to remove it?
@roomiemcgee8899
@roomiemcgee8899 Жыл бұрын
I love how early 2000's this video feels
@Yhusi
@Yhusi Жыл бұрын
Thanks for the nice video. Lately sometime I fail to make perfect well, and I realize it when I load my sample it won’t go through coz there are this sheet of the gel between the well. Might anyone have a suggestion? Many thanks
@funny11744
@funny11744 Жыл бұрын
I do not see a sterile process ! The tape is already contaminated with ambient contaminants, So how to apply perfectly sterile ? Perfectly sterile. because the tape is not sterile as we can see. I need an idea!
@greylingful
@greylingful Жыл бұрын
You have a very good video. 2 things that would set you apart from others...1. list the glassware and tell where we can purchase. 2. Answer peoples questions. Those who ignore simple questions are only in it for themselves and I will not give any likes or watch any more of a persons work if they are so selfish.
@raihanuddin9285
@raihanuddin9285 Жыл бұрын
Thanks mate ❤️
@divya-c6v
@divya-c6v Жыл бұрын
thanks 🙏🏻
@fatmahmansi4291
@fatmahmansi4291 Жыл бұрын
Thank you for stopping my tears 😩
@meenakshichugh9207
@meenakshichugh9207 Жыл бұрын
I had tried to filter the buffer through the same glass assembly, but I was facing a leakage problem from the filter base and funnel. I had tried so much to fix it but couldn't do so. Is there any technique to assemble the funnel and base with a clamp? Can you please help me out?
@Deepak-lq8xt
@Deepak-lq8xt Жыл бұрын
Thanks
@oladelejimoh2062
@oladelejimoh2062 Жыл бұрын
Hi cations and anions, While loading my samples, the samples did not enter into the well but floated... Can you help me troubleshoot this? Thanks
@adebowalealade4648
@adebowalealade4648 2 жыл бұрын
Hi, I just came across this channel and The Lab channel. You guys are doing a fantastic and amazing job. However, what happened? Where have you been? It's been 10 years.
@gustavowfringow805
@gustavowfringow805 2 жыл бұрын
Can you please explain how to tell which side up for a 20micron membrane filter, thanks 🙏
@aakarshahuja3777
@aakarshahuja3777 2 жыл бұрын
PLEASE MAKE VIDEOS ON THE PROCESSES OF RECOMBINANT DNA TECHNOLOGY ?
@aakarshahuja3777
@aakarshahuja3777 2 жыл бұрын
WHAT IS REPORTER GENE ?
@aakarshahuja3777
@aakarshahuja3777 2 жыл бұрын
HOW DO WE KNOW CELL HAS BEEN TRANSFORMED ?
@aakarshahuja3777
@aakarshahuja3777 2 жыл бұрын
WHAT IS THE QUALIFICATION REQUIRED FOR THIS JOB , WHAT ARE THE PROSPECTS OF THIS AND WHAT ARE THE PRODUCTS THAT CAN BE MADE ?
@zahidbashir8946
@zahidbashir8946 2 жыл бұрын
Have u got any protocol for prepration of the reagents.
@panoskre
@panoskre 2 жыл бұрын
Thank you!
@dhanumurasing6292
@dhanumurasing6292 2 жыл бұрын
Thank you brother... helpful for me
@s.foudehi1419
@s.foudehi1419 2 жыл бұрын
Thank you so much. I wasted so much parafilm, i thought you would need a big piece xD
@succeed_with_me_SSC
@succeed_with_me_SSC 2 жыл бұрын
Legends are here from 2022__😂😂
@betaselshartford9454
@betaselshartford9454 2 жыл бұрын
How much is centrifuge
@p.m.satyanarayana8505
@p.m.satyanarayana8505 2 жыл бұрын
I saw my gel getting shrunk after staining & destaining (CBB). Staining- 10 mins- generally I stain it for 10 mins & destaining-30 mins.
@p.m.satyanarayana8505
@p.m.satyanarayana8505 2 жыл бұрын
I have been staining & destaining for 10 mins and add water & leave it overnight. Bands always were visible clearly.
@ahmadmuhaiminbinmohdzulia2230
@ahmadmuhaiminbinmohdzulia2230 2 жыл бұрын
Hi. I hv 2 questions. Why add isopropanol to removes bubbles and why is there stacking and resolving gel ?