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Пікірлер
@anushreedastidar4129
@anushreedastidar4129 7 ай бұрын
Hi, what is the bioink and fresh bath material being used?
@yuyjhtybgfdrvj5263
@yuyjhtybgfdrvj5263 8 ай бұрын
Thank you for this great video, can you please tell me what is the needle specifications like diameter, length and etc.
@yuyjhtybgfdrvj5263
@yuyjhtybgfdrvj5263 8 ай бұрын
Hi, for liquid-like solution how much needle diameter do we need?
@iteleshov
@iteleshov Жыл бұрын
Cool video, thanks!
@AbdulAhad-qk1iu
@AbdulAhad-qk1iu Жыл бұрын
Hi dear! Which polymer is best for bone scaffold via 3d bioprinting? It means scaffold little bit hard after printing
@BernhardRieder1
@BernhardRieder1 Жыл бұрын
Who can I connect with at Stanford? I am almost every week on campus and would love to meet makers and bioprinting reserachers and developers. Appreciate any additional information.
@BeautifulPeopleBTFLPPL
@BeautifulPeopleBTFLPPL 2 жыл бұрын
Will 3D Bioprinting lead us to a future with printed organs and tissues? kzbin.info/www/bejne/ioOylIdum7VgY6M ......
@Ste5194
@Ste5194 2 жыл бұрын
Hello, i have some questions about the same protocol (Fresh + alginate) with cells. Can I use the same temperatures (plate <23*C and needle <10*C ) if I add a cells pellet in alginate solution?
@ilasingh729
@ilasingh729 2 жыл бұрын
how to increase bed temperature
2 жыл бұрын
Do you have a paper of this?
@3dpolygon962
@3dpolygon962 2 жыл бұрын
I've made a video in my channel about leaders of bioprinting companies in 2021
@mohandey591
@mohandey591 2 жыл бұрын
What is storage protocol for remaining GelMa, Hydrogel?
@3ducators641
@3ducators641 2 жыл бұрын
What material is this printing with? Super flexible
@mohandey591
@mohandey591 2 жыл бұрын
Hello, I am a new user of Allevi Bioprinter. I have previous experience of 3D printer, which was mainly DLP based. And I worked as a support team member(3D printer training, servicing, maintenance and quality control). Recently, I have started work on Allevi Bioprinter for my research study(as a PhD student). It is a great Bioprinter. Now I want to know, Where can I get support for some of my queries? Thank you so much.
@amarhussain4145
@amarhussain4145 2 жыл бұрын
3D Bioprinting for Medical Applications kzbin.info/www/bejne/gKXJg3Rsp7iJjNE
@TuhinBepariMusic
@TuhinBepariMusic 2 жыл бұрын
Wow.. It's awesome😊👍
@sherazahmed223
@sherazahmed223 2 жыл бұрын
hello, I am facing problems in crosslinking of alginate structure and washing/removing gelatin. Please if you could help me in the crosslinking of alginate, concentration or temperature.
@Ste5194
@Ste5194 2 жыл бұрын
I change the alginate powder and the sample are the best😊
@이라라-c7m
@이라라-c7m Жыл бұрын
@@Ste5194 to what alginate powder did you change?
@happyandhealthy888
@happyandhealthy888 2 жыл бұрын
look it my thing
@ridwanulislam2511
@ridwanulislam2511 3 жыл бұрын
Have anyone told you Yakub that you look like Jurgen Klopp ?
@bhujampavlog9366
@bhujampavlog9366 3 жыл бұрын
Ha ha ha that true
@hassaan5809
@hassaan5809 3 жыл бұрын
Wouldn't be surprised if, in a few decades, whole departments and industries are built on this technology!
@LorenzKamo
@LorenzKamo 3 жыл бұрын
Congrats on being part of 3D Systems
@naushadturja6547
@naushadturja6547 3 жыл бұрын
Do you have any study material or manual to make a 3d bioprinter for own research? For my project work I have build a 3d bioprinter with a cheap cost. Can u help?
@alleviinc6923
@alleviinc6923 3 жыл бұрын
Allevi 3D bioprinters come in a wide range of prices to fit the needs of lab budget's both large and small. Email us at [email protected] to receive a custom quote for your lab. You should also definitely check out our protocols page (www.allevi3d.com/protocols/) which has lots of great articles for 3D cell culture. Let us know if you have any additional questions!
@ericb3able
@ericb3able 3 жыл бұрын
Buy an i3 clone, modify the printhead to accept a custom or off-the-shelf syringe pump (probably won't have temp control) and start experimenting with Extrusion rates. Alternatively, build a heating block that can hold a syringe, then modify Marlin to run a vacuum pump as an extruder, substituting vacuum pressure steps for motor steps. It will require a lot of fine-tuning the extrusion rates for materials. Good luck!
@BeautifulPeopleBTFLPPL
@BeautifulPeopleBTFLPPL 2 жыл бұрын
Will 3D Bioprinting lead us to a future with printed organs and tissues? kzbin.info/www/bejne/ioOylIdum7VgY6M ......
@keithcorreia97
@keithcorreia97 3 жыл бұрын
Which soft materials are squeezed out of the syringe?
@alleviinc6923
@alleviinc6923 3 жыл бұрын
It's really up to you! Allevi users work with a wide range of industry-trusted bioinks (such as collagen, matrigel, gelma, and PLGA) as well as their own custom formulations. Most of our users work with a hydrogel mixed with whichever cell line they are working on. Please let us know if you have any additional questions. Thank you!
@keithcorreia97
@keithcorreia97 3 жыл бұрын
@@alleviinc6923 No but the video was really great and useful, and thank you fro explaining the materials inside :)
@aniksamiurrahman6365
@aniksamiurrahman6365 3 жыл бұрын
@@keithcorreia97 These are generally called hydrogel. Check it out if you are interested.
@keithcorreia97
@keithcorreia97 3 жыл бұрын
@@aniksamiurrahman6365 Ah thanks for that man!
@BeautifulPeopleBTFLPPL
@BeautifulPeopleBTFLPPL 2 жыл бұрын
Will 3D Bioprinting lead us to a future with printed organs and tissues? kzbin.info/www/bejne/ioOylIdum7VgY6M ......
@EverythingAndAnythingGoes24
@EverythingAndAnythingGoes24 3 жыл бұрын
Could it print fur?
@giuseppelauricella3306
@giuseppelauricella3306 4 жыл бұрын
Hi, where can I contact you?
@alleviinc6923
@alleviinc6923 4 жыл бұрын
Hi Giuseppe! You can contact us by emailing [email protected] and we'll connect you with a member of our team. Thanks!
@crosscarlisle3077
@crosscarlisle3077 4 жыл бұрын
Touched on a lot of tissue engineering concepts. Great stuff!
@reece417
@reece417 4 жыл бұрын
This is great information. The part about the collagen makes me excited for the future of 3d bioprinting .
@vipulthakkar1111
@vipulthakkar1111 4 жыл бұрын
I want nephornes and bio kidney sample please contact me and also iwant to oder this printer
@behzadheidari152
@behzadheidari152 4 жыл бұрын
Could you specify ingredients please?
@bitchoflivingblah
@bitchoflivingblah 4 жыл бұрын
LAP is the photoinitiator, which when activated by UV light will initiate crosslinking of the methycrylated (gelatin) collagen (GELMA). The company sells GELMA which is why they don't tell you how to make it. It is actually fairly easy to make GELMA if you have lab facilities at hand, a simple reaction, followed by dialysis and then finally lyophilization. Hope this helps.
@georgenaugles5039
@georgenaugles5039 4 жыл бұрын
Thank you for your video. Have you considered adding more information about trouble-shooting z calibration to address needle-breaks and repeated needle+petri plate contact with forces sufficient to break extruder needle?
@fx1c333
@fx1c333 4 жыл бұрын
By the time you get up to speed I would be dead. Every time there is a breakthrough it's always too far in the future. Pity my 3d printer can't do it. So a kidney if it's that Complex why not redesign it to be a simple print. It wouldn't have to be exact but functional to us who are awaiting the organs. Take a car engine complex machine been around for years , then you have electric car engines does the same job but simpler in design. ( get it )
@ravijain3858
@ravijain3858 5 жыл бұрын
Hi Please send me more details on my mail
@alleviinc6923
@alleviinc6923 5 жыл бұрын
Hi Ravi - please email us at [email protected] to discuss further. Thank you!
@alexjack9603
@alexjack9603 5 жыл бұрын
What nozzle diameter was used in the video?
@alleviinc6923
@alleviinc6923 5 жыл бұрын
Hi Alex - this is a 28(22) gauge tip. Please let us know if you have any additional questions. We can be reached 24/7 by emailing us at [email protected]. Thank you!
@chloedevillard6974
@chloedevillard6974 5 жыл бұрын
What speed do you use for this print ?
@alleviinc6923
@alleviinc6923 5 жыл бұрын
Hi Chloe! We printed this structure at 6mm/s. Please let us know if you have any additional questions. Thank you!
@louisesaillard7634
@louisesaillard7634 5 жыл бұрын
Could you specify your ingredients please ?
@courtneypopp8578
@courtneypopp8578 5 жыл бұрын
This is awesome and very applicable!!
@mariadln777
@mariadln777 6 жыл бұрын
Awesome use of medical technology to help those in need of it.
@dsazer25
@dsazer25 6 жыл бұрын
👍👍👍
@FranciscoMartinez-et9kz
@FranciscoMartinez-et9kz 6 жыл бұрын
What type of nozzle and at which temperature is GelMA 10% being printed?
@alleviinc6923
@alleviinc6923 6 жыл бұрын
Hi Francisco! In this video, we are printing with a 27 gauge tapered needle (335 um inner diameter) at room temperature. Let us know if you have any other questions!
@naveenravindar
@naveenravindar 6 жыл бұрын
were you able to remove the structure?
@mhwinter22
@mhwinter22 6 жыл бұрын
Hi Naveen - Yes! You wash away the FRESH gelatin slurry and then can suspend structure in PBS. Please let us know if you have any additional questions. Thanks!
@naveenravindar
@naveenravindar 6 жыл бұрын
mhwinter22 What alginate and CaCl2 concentration did you use?
@Dispensecom
@Dispensecom 6 жыл бұрын
where in the process did the carbohydrate glass dissolve?
@alleviinc6923
@alleviinc6923 6 жыл бұрын
During the first perfusion (with either IPA or warm water) the carbohydrate glass is dissolved and cleared from the channels. Let us know if you have any additional questions!
@dsazer25
@dsazer25 6 жыл бұрын
Wow! This is super cool.
@spencer00andrew
@spencer00andrew 6 жыл бұрын
How did you make the Gcode to print out-of-plane (i.e. initiate movement only in the Z-direction)?
@alleviinc6923
@alleviinc6923 6 жыл бұрын
Hey Andrew, This is a feature of the new Allevi Software slated to be released in June. Please let me know if you have any questions or if you'd like to connect to learn more!
@pshankles3
@pshankles3 6 жыл бұрын
We just published on a similar method using an FDM 3D printer to create molds. I like the use of carbohydrate glass to dissolve away the mold journals.plos.org/plosone/article?id=10.1371/journal.pone.0192752
@alleviinc6923
@alleviinc6923 6 жыл бұрын
Hey Peter, super cool. Your y-junction is so clean! And yes, we really like the carbohydrate glass given the inner chip architecture we can obtain and the casting freedom. Thanks for sharing your paper!
@entlearning70
@entlearning70 6 жыл бұрын
How you change the color in Pluronic? what kind of material you use for this?
@alleviinc6923
@alleviinc6923 6 жыл бұрын
Great question! We added food dye for the video so the pluronic would be easier to see. Let us know if you have any further questions!