Hi, what is the bioink and fresh bath material being used?
@yuyjhtybgfdrvj52638 ай бұрын
Thank you for this great video, can you please tell me what is the needle specifications like diameter, length and etc.
@yuyjhtybgfdrvj52638 ай бұрын
Hi, for liquid-like solution how much needle diameter do we need?
@iteleshov Жыл бұрын
Cool video, thanks!
@AbdulAhad-qk1iu Жыл бұрын
Hi dear! Which polymer is best for bone scaffold via 3d bioprinting? It means scaffold little bit hard after printing
@BernhardRieder1 Жыл бұрын
Who can I connect with at Stanford? I am almost every week on campus and would love to meet makers and bioprinting reserachers and developers. Appreciate any additional information.
@BeautifulPeopleBTFLPPL2 жыл бұрын
Will 3D Bioprinting lead us to a future with printed organs and tissues? kzbin.info/www/bejne/ioOylIdum7VgY6M ......
@Ste51942 жыл бұрын
Hello, i have some questions about the same protocol (Fresh + alginate) with cells. Can I use the same temperatures (plate <23*C and needle <10*C ) if I add a cells pellet in alginate solution?
@ilasingh7292 жыл бұрын
how to increase bed temperature
2 жыл бұрын
Do you have a paper of this?
@3dpolygon9622 жыл бұрын
I've made a video in my channel about leaders of bioprinting companies in 2021
@mohandey5912 жыл бұрын
What is storage protocol for remaining GelMa, Hydrogel?
@3ducators6412 жыл бұрын
What material is this printing with? Super flexible
@mohandey5912 жыл бұрын
Hello, I am a new user of Allevi Bioprinter. I have previous experience of 3D printer, which was mainly DLP based. And I worked as a support team member(3D printer training, servicing, maintenance and quality control). Recently, I have started work on Allevi Bioprinter for my research study(as a PhD student). It is a great Bioprinter. Now I want to know, Where can I get support for some of my queries? Thank you so much.
@amarhussain41452 жыл бұрын
3D Bioprinting for Medical Applications kzbin.info/www/bejne/gKXJg3Rsp7iJjNE
@TuhinBepariMusic2 жыл бұрын
Wow.. It's awesome😊👍
@sherazahmed2232 жыл бұрын
hello, I am facing problems in crosslinking of alginate structure and washing/removing gelatin. Please if you could help me in the crosslinking of alginate, concentration or temperature.
@Ste51942 жыл бұрын
I change the alginate powder and the sample are the best😊
@이라라-c7m Жыл бұрын
@@Ste5194 to what alginate powder did you change?
@happyandhealthy8882 жыл бұрын
look it my thing
@ridwanulislam25113 жыл бұрын
Have anyone told you Yakub that you look like Jurgen Klopp ?
@bhujampavlog93663 жыл бұрын
Ha ha ha that true
@hassaan58093 жыл бұрын
Wouldn't be surprised if, in a few decades, whole departments and industries are built on this technology!
@LorenzKamo3 жыл бұрын
Congrats on being part of 3D Systems
@naushadturja65473 жыл бұрын
Do you have any study material or manual to make a 3d bioprinter for own research? For my project work I have build a 3d bioprinter with a cheap cost. Can u help?
@alleviinc69233 жыл бұрын
Allevi 3D bioprinters come in a wide range of prices to fit the needs of lab budget's both large and small. Email us at [email protected] to receive a custom quote for your lab. You should also definitely check out our protocols page (www.allevi3d.com/protocols/) which has lots of great articles for 3D cell culture. Let us know if you have any additional questions!
@ericb3able3 жыл бұрын
Buy an i3 clone, modify the printhead to accept a custom or off-the-shelf syringe pump (probably won't have temp control) and start experimenting with Extrusion rates. Alternatively, build a heating block that can hold a syringe, then modify Marlin to run a vacuum pump as an extruder, substituting vacuum pressure steps for motor steps. It will require a lot of fine-tuning the extrusion rates for materials. Good luck!
@BeautifulPeopleBTFLPPL2 жыл бұрын
Will 3D Bioprinting lead us to a future with printed organs and tissues? kzbin.info/www/bejne/ioOylIdum7VgY6M ......
@keithcorreia973 жыл бұрын
Which soft materials are squeezed out of the syringe?
@alleviinc69233 жыл бұрын
It's really up to you! Allevi users work with a wide range of industry-trusted bioinks (such as collagen, matrigel, gelma, and PLGA) as well as their own custom formulations. Most of our users work with a hydrogel mixed with whichever cell line they are working on. Please let us know if you have any additional questions. Thank you!
@keithcorreia973 жыл бұрын
@@alleviinc6923 No but the video was really great and useful, and thank you fro explaining the materials inside :)
@aniksamiurrahman63653 жыл бұрын
@@keithcorreia97 These are generally called hydrogel. Check it out if you are interested.
@keithcorreia973 жыл бұрын
@@aniksamiurrahman6365 Ah thanks for that man!
@BeautifulPeopleBTFLPPL2 жыл бұрын
Will 3D Bioprinting lead us to a future with printed organs and tissues? kzbin.info/www/bejne/ioOylIdum7VgY6M ......
@EverythingAndAnythingGoes243 жыл бұрын
Could it print fur?
@giuseppelauricella33064 жыл бұрын
Hi, where can I contact you?
@alleviinc69234 жыл бұрын
Hi Giuseppe! You can contact us by emailing [email protected] and we'll connect you with a member of our team. Thanks!
@crosscarlisle30774 жыл бұрын
Touched on a lot of tissue engineering concepts. Great stuff!
@reece4174 жыл бұрын
This is great information. The part about the collagen makes me excited for the future of 3d bioprinting .
@vipulthakkar11114 жыл бұрын
I want nephornes and bio kidney sample please contact me and also iwant to oder this printer
@behzadheidari1524 жыл бұрын
Could you specify ingredients please?
@bitchoflivingblah4 жыл бұрын
LAP is the photoinitiator, which when activated by UV light will initiate crosslinking of the methycrylated (gelatin) collagen (GELMA). The company sells GELMA which is why they don't tell you how to make it. It is actually fairly easy to make GELMA if you have lab facilities at hand, a simple reaction, followed by dialysis and then finally lyophilization. Hope this helps.
@georgenaugles50394 жыл бұрын
Thank you for your video. Have you considered adding more information about trouble-shooting z calibration to address needle-breaks and repeated needle+petri plate contact with forces sufficient to break extruder needle?
@fx1c3334 жыл бұрын
By the time you get up to speed I would be dead. Every time there is a breakthrough it's always too far in the future. Pity my 3d printer can't do it. So a kidney if it's that Complex why not redesign it to be a simple print. It wouldn't have to be exact but functional to us who are awaiting the organs. Take a car engine complex machine been around for years , then you have electric car engines does the same job but simpler in design. ( get it )
@ravijain38585 жыл бұрын
Hi Please send me more details on my mail
@alleviinc69235 жыл бұрын
Hi Ravi - please email us at [email protected] to discuss further. Thank you!
@alexjack96035 жыл бұрын
What nozzle diameter was used in the video?
@alleviinc69235 жыл бұрын
Hi Alex - this is a 28(22) gauge tip. Please let us know if you have any additional questions. We can be reached 24/7 by emailing us at [email protected]. Thank you!
@chloedevillard69745 жыл бұрын
What speed do you use for this print ?
@alleviinc69235 жыл бұрын
Hi Chloe! We printed this structure at 6mm/s. Please let us know if you have any additional questions. Thank you!
@louisesaillard76345 жыл бұрын
Could you specify your ingredients please ?
@courtneypopp85785 жыл бұрын
This is awesome and very applicable!!
@mariadln7776 жыл бұрын
Awesome use of medical technology to help those in need of it.
@dsazer256 жыл бұрын
👍👍👍
@FranciscoMartinez-et9kz6 жыл бұрын
What type of nozzle and at which temperature is GelMA 10% being printed?
@alleviinc69236 жыл бұрын
Hi Francisco! In this video, we are printing with a 27 gauge tapered needle (335 um inner diameter) at room temperature. Let us know if you have any other questions!
@naveenravindar6 жыл бұрын
were you able to remove the structure?
@mhwinter226 жыл бұрын
Hi Naveen - Yes! You wash away the FRESH gelatin slurry and then can suspend structure in PBS. Please let us know if you have any additional questions. Thanks!
@naveenravindar6 жыл бұрын
mhwinter22 What alginate and CaCl2 concentration did you use?
@Dispensecom6 жыл бұрын
where in the process did the carbohydrate glass dissolve?
@alleviinc69236 жыл бұрын
During the first perfusion (with either IPA or warm water) the carbohydrate glass is dissolved and cleared from the channels. Let us know if you have any additional questions!
@dsazer256 жыл бұрын
Wow! This is super cool.
@spencer00andrew6 жыл бұрын
How did you make the Gcode to print out-of-plane (i.e. initiate movement only in the Z-direction)?
@alleviinc69236 жыл бұрын
Hey Andrew, This is a feature of the new Allevi Software slated to be released in June. Please let me know if you have any questions or if you'd like to connect to learn more!
@pshankles36 жыл бұрын
We just published on a similar method using an FDM 3D printer to create molds. I like the use of carbohydrate glass to dissolve away the mold journals.plos.org/plosone/article?id=10.1371/journal.pone.0192752
@alleviinc69236 жыл бұрын
Hey Peter, super cool. Your y-junction is so clean! And yes, we really like the carbohydrate glass given the inner chip architecture we can obtain and the casting freedom. Thanks for sharing your paper!
@entlearning706 жыл бұрын
How you change the color in Pluronic? what kind of material you use for this?
@alleviinc69236 жыл бұрын
Great question! We added food dye for the video so the pluronic would be easier to see. Let us know if you have any further questions!