I really like this course！！！

You are making amazing video content. I just watched the video and your video quality is appreciable. I am so impressed by your content, I was analyzing your channel and noticed that You are struggling with video views and video ranking. May I share some tips on how you can achieve your goals?

These discussion ultimately resulted in a community white paper: Gatto, L., Aebersold, R., Cox, J. et al. Initial recommendations for performing, benchmarking and reporting single-cell proteomics experiments. Nat Methods 20, 375-386 (2023). doi.org/10.1038/s41592-023-01785-3 Website: single-cell.net/guidelines

gromacs?

what is being exchanged?

como se guarda el video en vmd?

unholy

to be honest, i never even knew.

Interesting

They also can turn deer and elk into zombies👍

As a guy who constantly change positions in bed to get comfortable I relate to this.

Thank you,, i almost forgot!

thank you for the reminder. it is highly appreciated

Wouldn't know what I'd have done if I forgot that for a second. Thanks!

I had almost forgotten, thank you

Amazing work!

what is the difference between relative protein quantification and absolute protein quantification?

Thanks for sharing it.

Having trouble finding the follow up videos for this series, do they in fact exist?

Why only 240p? Unfortunately looks like pixel salad

🙏🏼🙏🏼

thx man

lol! Great ad

Nikolai, is this a better approach in relation to Nautilus Biotechnology platform? How do you expect the throughput and quality of protein identification?

based, excelent video.

The first article about patch-clamp integration with MS / proteomics: O. Gradov and M. Gradova, "MS-patch-clamp or the possibility of mass spectrometry hybridization with patch-clamp setups for single cell metabolomics and channelomics", Advances in Biochemistry 3 (2015), 66-71. This paper was cited in: Zhu, Hongying, Qianqian Li, Tiepeng Liao, Xiang Yin, Qi Chen, Ziyi Wang, Meifang Dai et al. "Metabolomic profiling of single enlarged lysosomes." Nature Methods 18, no. 7 (2021): 788-798 (experimental paper, realized so beautiful ideas) and Zhang, Linwen, and Akos Vertes. "Single‐cell mass spectrometry approaches to explore cellular heterogeneity." Angewandte Chemie International Edition 57, no. 17 (2018): 4466-4477. {Zhang, Linwen, and Akos Vertes. "Einzelzell‐Massenspektrometrie zur Untersuchung zellulärer Heterogenität." Angewandte Chemie 130, no. 17 (2018): 4554-4566}. The first proposal of this approach (unrealized in destroyed russian infrastructure under the pressing of totalitarian science organization in Russia): Gradov O.V., Gradova M.A. (2014). On the possibility of "MS-patch-clamp" or mass spectrometry hybridization with patch-clamp setups for single cell metabolomics and channelomics. In: Proc. Int. Workshop “Structure and Functions of Biomembranes” (29 Sept. - 3 Oct. 2014; Moscow Institute of Physics and Technology), 105-106. I am very glad and happy to see so beautiful works in the Prof. Meni Wanunu presentation. I think, that it is very perspective research branch. Best regards, O.V. Gradov, senior reseracher.

Fantastic presentation & transformative technology )

What biology are we learning? The section starts at 16:27

Fantastic presentation!

A primer and current trends in single-molecule proteomics: 22:30

Current trends in single-cell mass spec proteomics: 8:54

Introduction to SCP2023 and logistics: 0:33

The first article about patch-clamp integration with MS / proteomics: O. Gradov and M. Gradova, "MS-patch-clamp or the possibility of mass spectrometry hybridization with patch-clamp setups for single cell metabolomics and channelomics", Advances in Biochemistry 3 (2015), 66-71. This paper was cited in: Zhu, Hongying, Qianqian Li, Tiepeng Liao, Xiang Yin, Qi Chen, Ziyi Wang, Meifang Dai et al. "Metabolomic profiling of single enlarged lysosomes." Nature Methods 18, no. 7 (2021): 788-798 (very beautiful experimental paper, realized so original ideas) and Zhang, Linwen, and Akos Vertes. "Single‐cell mass spectrometry approaches to explore cellular heterogeneity." Angewandte Chemie International Edition 57, no. 17 (2018): 4466-4477. {Zhang, Linwen, and Akos Vertes. "Einzelzell‐Massenspektrometrie zur Untersuchung zellulärer Heterogenität." Angewandte Chemie 130, no. 17 (2018): 4554-4566}. The first proposal of this approach (unrealized in destroyed russian infrastructure under the pressing of totalitarian science organization in Russia): Gradov O.V., Gradova M.A. (2014). On the possibility of "MS-patch-clamp" or mass spectrometry hybridization with patch-clamp setups for single cell metabolomics and channelomics. In: Proc. Int. Workshop “Structure and Functions of Biomembranes” (29 Sept. - 3 Oct. 2014; Moscow Institute of Physics and Technology), 105-106. I am very glad and happy to see so beautiful works in the Slavov Lab, which can be intepreted as one of the most respectable laboratory in this branch of science (or R&D). Best regards, O.V. Gradov.

The first article about patch-clamp integration with MS / proteomics: O. Gradov and M. Gradova, "MS-patch-clamp or the possibility of mass spectrometry hybridization with patch-clamp setups for single cell metabolomics and channelomics", Advances in Biochemistry 3 (2015), 66-71. This paper was cited in: Zhu, Hongying, Qianqian Li, Tiepeng Liao, Xiang Yin, Qi Chen, Ziyi Wang, Meifang Dai et al. "Metabolomic profiling of single enlarged lysosomes." Nature Methods 18, no. 7 (2021): 788-798 (experimental paper, realized so beautiful ideas) and Zhang, Linwen, and Akos Vertes. "Single‐cell mass spectrometry approaches to explore cellular heterogeneity." Angewandte Chemie International Edition 57, no. 17 (2018): 4466-4477. {Zhang, Linwen, and Akos Vertes. "Einzelzell‐Massenspektrometrie zur Untersuchung zellulärer Heterogenität." Angewandte Chemie 130, no. 17 (2018): 4554-4566}. The first proposal of this approach (unrealized in destroyed russian infrastructure under the pressing of totalitarian science organization in Russia): Gradov O.V., Gradova M.A. (2014). On the possibility of "MS-patch-clamp" or mass spectrometry hybridization with patch-clamp setups for single cell metabolomics and channelomics. In: Proc. Int. Workshop “Structure and Functions of Biomembranes” (29 Sept. - 3 Oct. 2014; Moscow Institute of Physics and Technology), 105-106. I am very glad and happy to see so beautiful works in the Slavov Lab, which can be intepreted as one of the most respectable laboratory in this branch of science (or R&D). Best regards, O.V. Gradov.

The first article about patch-clamp integration with MS / proteomics: O. Gradov and M. Gradova, "MS-patch-clamp or the possibility of mass spectrometry hybridization with patch-clamp setups for single cell metabolomics and channelomics", Advances in Biochemistry 3 (2015), 66-71. This paper was cited in: Zhu, Hongying, Qianqian Li, Tiepeng Liao, Xiang Yin, Qi Chen, Ziyi Wang, Meifang Dai et al. "Metabolomic profiling of single enlarged lysosomes." Nature Methods 18, no. 7 (2021): 788-798 (experimental paper, realized so beautiful ideas) and Zhang, Linwen, and Akos Vertes. "Single‐cell mass spectrometry approaches to explore cellular heterogeneity." Angewandte Chemie International Edition 57, no. 17 (2018): 4466-4477. {Zhang, Linwen, and Akos Vertes. "Einzelzell‐Massenspektrometrie zur Untersuchung zellulärer Heterogenität." Angewandte Chemie 130, no. 17 (2018): 4554-4566}. The first proposal of this approach (unrealized in destroyed russian infrastructure under the pressing of totalitarian science organization in Russia): Gradov O.V., Gradova M.A. (2014). On the possibility of "MS-patch-clamp" or mass spectrometry hybridization with patch-clamp setups for single cell metabolomics and channelomics. In: Proc. Int. Workshop “Structure and Functions of Biomembranes” (29 Sept. - 3 Oct. 2014; Moscow Institute of Physics and Technology), 105-106. I am very glad and happy to see so beautiful works in the Slavov Lab, which can be intepreted as one of the most respectable laboratory in this branch of science (or R&D).

It's important to keep in mind that the purpose of explaining something is to help others understand, not to showcase our own understanding. By using simple language and clear explanations, we can make the information more accessible and easier to understand for everyone. It's also important to remember that not everyone has the same level of knowledge or expertise, so we should tailor our explanations to our audience. Effective communication is about helping others learn and grow, not about impressing them with our own intelligence

Interesting perspective coming from an elitist lab

13:09

3:25 theories 9:45 addiction 13:00 patch punch

2:55 8:20

Ok this is def one of the more INTERPRET-ABLE vids by you

What you offer is really great. Maybe I cannot fully understand everything because it is not my specialty, but I am really interested in mass spectrometry and what you offer. Thank you You are the best

For more information on this method see: scp.slavovlab.net/nPOP and genomebiology.biomedcentral.com/articles/10.1186/s13059-022-02817-5

this is a wonder presentation.

2:30 is hilarious

Thanks ever so much for uploading this. Very interesting talk and what an amazing technique.

Mode details can be found in Derks, J. et al. Increasing the throughput of sensitive proteomics by plexDIA. Nat Biotechnol (2022). doi.org/10.1038/s41587-022-01389-w. The search is described in the methods section www.nature.com/articles/s41587-022-01389-w#Sec12

where are the .pipeline files that's mentioned in the tutorial?

Thank you, Sir. It is much needed. Can you make lecture on plant metabolite data analysis? Please do the needful. Thank you

Slides are available at: plexdia.slavovlab.net/mass-spec/Searching-plexDIA-data-with-DIA-NN.pdf