Maybe restoration of mammary involution signals could be dev'd as a therapeutic

Foxm1 inhibitors prevent formation of polyploid giant cancer cells, a preposed source of recurring cancer..It would be interesting to see PR, ER activity status in BC PGCCs

I did conditional ko in mouse MG w Diego Castrllon's flowed foxo1 mouse and saw delayed involution Ko of foxo1 + overexpression myc caused differentiation of tumors frm ER neg adenocarcinoma to papillary ER pos dcis

I think progesterone receptor has a duality w regard to use of antagonist vs agonists, however..

Some interesting publications are coming out on progesterone controling immune tolerance or evasion in cancer

Incredible

Tysm!

"How do you pronounce it" "Nobody believes in enhancer anymore" =)))) Your podcast is so real and friendly. Thanks for making all these podcast!

I have an Interesting Huntington situation and no one is following me. Are Any of you interested? Please leave contact info Thank you RC

Thank you!

"responsive chromatin becomes more permissive and cryptic transcription sites"

Very inspirational talk!

This was so eloquent!

Thank you so much for sharing this! Really helped me understand how single cell multiome works

3:30 epigenome sequence map 15:00 🐭🧠 18:00 cell map 24:30 29:00

4:30 psychencode

"enhanced mono-ADP ribosylation activity"

'Promo SM'

Ok...great...so how can I wake up my sirt 6 w seaweed

View Epigenetics Podcasts, Webinars, and access helpful application guides at ActiveMotif.com/resources

Thanks you. Seem as if the Liver is extremely important to overall aging, Would be nice to know if normal blood work (liver enzymes AST, ALT, GGT) correlated to epigenetic age.

What!? Scientists cannot study the rejuvenation event in human embryos because it happens just after the 14 day limit that requires the embryos to be destroyed? Does the same limit exist in Europe? So it's better for everyone to die then to keep an embryo around for maybe 30 days?

PIXUL Multi-Sample Sonicator Achieve truly reproducible shearing of chromatin, DNA, RNA, and protein. WEBSITE: www.activemotif.com/catalog/1300/pixul-multi-sample-sonicator Next-generation sequencing applications, such as ChIP-Seq, RNA-Seq, RIP-Seq, exome sequencing, and whole genome sequencing require precise sample fragmentation (200-600 bp) for compatibility with short read sequencing platforms. Shearing by physical sonication, as opposed to enzymatic methods, is preferred for its unbiased nature and ability to produce a relatively narrow fragmentation profile. However, most currently available sonication systems are incompatible with high-throughput sample processing or require expensive proprietary plates or tubes. PIXUL is the first and only multi-sample sonicator that delivers extremely consistent shearing of up to 96 samples processed in parallel and is fast, simple, and inexpensive to operate. PIXUL Multi-Sample Sonicator Highlights: Consistent Consistent: Process 1-96 samples simultaneously with extremely high reproducibility. Simple Simple: Fast setup, short learning curve, and easy operation with intuitive touchscreen. Fast Fast: Arrayed transducers and no degassing required saves time. Flexible Flexible: Use up to 12 different sonication conditions per run in 96-well plates for simultaneous processing of DNA, RNA, and protein sample types. Affordable Affordable: No requirement for buying accessories or expensive proprietary plates or tubes. Unsurpassed Sonication Consistency at an Affordable Operating Cost High-throughput sonication on other platforms is currently limited by the cost of consumables (expensive proprietary tubes, plates, and reagents). However, the low cost, round-bottom plates used with the PIXUL Multi-Sample Sonicator are directly compatible with cell culture and shearing, eliminating sample transfer steps that are inconvenient and can lead to sample loss. Furthermore, the Coupling Fluid used in the PIXUL instrument does not require lengthy degassing and can be adequately circulated and ready for sonication in approximately 15 minutes. The sonication run itself for up to 96 samples takes just 10-30 minutes, depending on the sample type and application.

PIXUL Multi-Sample Sonicator Achieve truly reproducible shearing of chromatin, DNA, RNA, and protein. WEBSITE: www.activemotif.com/catalog/1300/pixul-multi-sample-sonicator Next-generation sequencing applications, such as ChIP-Seq, RNA-Seq, RIP-Seq, exome sequencing, and whole genome sequencing require precise sample fragmentation (200-600 bp) for compatibility with short read sequencing platforms. Shearing by physical sonication, as opposed to enzymatic methods, is preferred for its unbiased nature and ability to produce a relatively narrow fragmentation profile. However, most currently available sonication systems are incompatible with high-throughput sample processing or require expensive proprietary plates or tubes. PIXUL is the first and only multi-sample sonicator that delivers extremely consistent shearing of up to 96 samples processed in parallel and is fast, simple, and inexpensive to operate. PIXUL Multi-Sample Sonicator Highlights: Consistent Consistent: Process 1-96 samples simultaneously with extremely high reproducibility. Simple Simple: Fast setup, short learning curve, and easy operation with intuitive touchscreen. Fast Fast: Arrayed transducers and no degassing required saves time. Flexible Flexible: Use up to 12 different sonication conditions per run in 96-well plates for simultaneous processing of DNA, RNA, and protein sample types. Affordable Affordable: No requirement for buying accessories or expensive proprietary plates or tubes. Unsurpassed Sonication Consistency at an Affordable Operating Cost High-throughput sonication on other platforms is currently limited by the cost of consumables (expensive proprietary tubes, plates, and reagents). However, the low cost, round-bottom plates used with the PIXUL Multi-Sample Sonicator are directly compatible with cell culture and shearing, eliminating sample transfer steps that are inconvenient and can lead to sample loss. Furthermore, the Coupling Fluid used in the PIXUL instrument does not require lengthy degassing and can be adequately circulated and ready for sonication in approximately 15 minutes. The sonication run itself for up to 96 samples takes just 10-30 minutes, depending on the sample type and application.

Thank you !!

is fucoidan a mix of several similar chemicals? Is it known which one has the strongest effect on activating sirt6? Thank you!

Very clear, Thanks!

Please add links to the articles that are discussed in these talks. Thank you.

Really inspiring work. It is great to know the time scale of these large molecules

Thank you for sharing. Exciting topic.

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View CUT&Tag-IT Assay Kits for Cells and Tissue samples, from Active Motif! www.activemotif.com/catalog/1368/cut-tag-it-kits

View RAS GTPase and Active KRAS ELISA Kits from Active Motif! www.activemotif.com/catalog/1364/ras-gtpase-products

Schedule a PIXUL Demo for your lab! www.activemotif.com/catalog/1300/pixul-multi-sample-sonicator

Schedule a PIXUL Demo for your lab! www.activemotif.com/catalog/1300/pixul-multi-sample-sonicator

Hello, my lab decided to try your kit of CUT&Tag for a transcription factor. Have you had any updated on this application of CUT&Tag?

Thank you for this great video!

Such an interesting lecture. Great work!

Thank you for sharing.

A very nice conference and a wonderful lecture. Thank you very much for sharing the knowledge.

Hi, thanks for the great lecture! Could u plz explain what non-base resolution and base resolution to me? Thank u so much!

awesome video for beginners

awesome

I want to do atac-seq so bad!

Gorkin. Nice.

Really wish the bioinformatics part spent more time focusing on the workflow (like it does at 41:10) than it does on introducing the platform. There's so much to expand upon here. 1. What's bugging me a bit here though is that if the expected insert sizes are in the 10-700 range, wouldn't the read data necessarily include adapter content at the 3' ends for shorter insert lengths? Unless your demultiplexing is trimming the adapters by default, you'd probably still need to run these through a trimmer, right? 2. Given the high similarity of the reads (the no dedup required bit), can we use that duplication to polish the peaks for motif analysis?

Does someone understand which pathway he mentions at 19:40? I don`t understand it acoustically.

Great webinar, thank you very much!

Very useful information! Thanks a lot