She could have used the spiderman 1 school outing explaination....

Pretending at home just like they do at the lab

i want whale myoglobin

Are the tips reuseable after boil sterilization

This has scary practical application in weapons tech

I am a MS student and want to do research in Molecular biology and Crisper cas9 is most publishing topic these days thank you Ami li

very cool))) you can try to incubate the transformed cells in liquid LB medium for 1 hour 37°. Then plate these cells on LB agar with antibiotic. (video instructions for transforming the dh5 Alpha are on the NEB website) and you will succeed!!!

Does this experiment explain why you haven't posted anything in years?... are you still around? 😂

Iam from India can you help to on gene editing on shrimp 🦐🦐

Y no se contaminó nada 😢??

It is best to order your plasmid with the promoter, multiple cloning site, antibiotic resistance gene and selectable marker and gene of interest already in it. All you will have to do is make the Agar and carry out the cell transformation.

I am a molecular biology and genetics student, and this is very helpful for writing the lab protocol/process. Thanks for sharing ❤

Keep posting! Great experiment! I was born with PKD, a genetic disease, and 4 weeks ago I had my kidneys removed. So, for me, science is the only way I survive today (dialysis) and that will enable me to survive tomorrow and keep loving my family!

YES ANTIBIOTIC RESISTANSE PLAGUE!!!!

That's good

Can you use this to make antibiotics at home?

Failure is the mother of knowledge, keep it up! You're doing great!

"... and the bottle cracked". The antibiotics reached into the environment and led to some bacteria resistant to this particular antibiotic. These bacteria found their way into the wounds of people who were about to go into hospital due to another bacterial infection. They could not be treated successfully and eventually died. That's why experiments like this one should NOT BE PERFORMED by ordinary people who do not know what the consequences of their actions are or do not have the appropriate security equipment to minimize such side effects.

what is it that was meant to happen and what was it that should have made it happen?

Snow is actually insulating. It's mostly air.

Nice! I gotta say your streaking technique is something new to me.

I came for “mad scientist at home”, I’m staying & subbing for the sound effects when pouring agar into dishes. 😂😂😂

I was pondering the agar from the Chinese grocery store 😂😂😂 instead of pharma grade agar

How cool is that? CRIPR at home, hope you make more videos like this :D I am also building some sort of biolab at home xD it will be good if you share some ideas :D TYVM for sharing

Just watched your video with my gr 12 bio students! thanks for showing us that science can be done at home and double thanks for showing us that mistakes are real! Keep going!

It didnt occur to me how accessible this stuff was until I saw someone puriify cas 9 in the lab. I was like "ow yh its just a protein" , very scary but exciting moment :)

Great video, science rules!!

I've been want to get into this.

Thank you

OMG it's that SIMPLE!??!??!! I'm sure it will succeed if she got a temperature-stabilized incubator.

Your video is just awesomeeeee...!❤✨ But will you please add subtitles in it.

Can I use crispr cas9 to modify baceria to produce pure meth? Just asking. For science.

Did you know that that Peg is causing inflammation in body and now people are trying to figure out how to get rid of that duct tape it's a polymer your body cannot degrade it it's just causing bigger and bigger inflammation that killer T cells are dying this Peg are lipid nano fats and the sales for creating antibodies are exhausting

I’ve seen a lot of people use crispr on bacteria but I’m curious if it’s possible to edit the genes of a multicellular organisms, such as plants, at home?

That was an awesome video. Extremely fascinating.

I want to have the original bananas back.

So, you made an antibióticos resistant bacteria? Are you Crazy ?

You earned a sub literally the best work I have seen and realllyyyyyyyy nicely presented and made things simple

yaaaas queen

this was amazing🥳🥳🥳🥳next time plz also show us the bacteria under the microscope

Hey Amy! thank you for this great video, I'm a highschooler who wants to conduct similar experiments at home, may I inquire you the total price of those equipments and where you got them from? thank you

Fantastic 🤓

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I'm building my own genetics lab at home also!!!!¡¡!!!!!

Thank you. Please do it again.

Fascinating video!

John 3:16 KJV For God so loved the world, that he gave his only begotten Son, that whosoever believeth in him should not perish, but have everlasting life.

John 3:16 KJV For God so loved the world, that he gave his only begotten Son, that whosoever believeth in him should not perish, but have everlasting life.

Did u kill all nacteria when done