Neil Cross

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@dimarxc Ай бұрын

I wish I can have a lecturer like you

@amlmohamedelshabasy Ай бұрын

THANK YOU VERY MUCH I REALLY APPRECIATE THAT

@albinaamangaliyeva756 Ай бұрын

This is so helpful! Thank you so much!

@nikinajafi3823 3 ай бұрын

Really helpful thanks! I needed those tips

@alethea1158 4 ай бұрын

This video was very helpful, thank you so much!

@home4429 4 ай бұрын

Super informative, thank you so much!

@joel2757 4 ай бұрын

Great tutorial; it helped me to remember some basic stuff!!

@psirer..9625 5 ай бұрын

Thank you for the content.

@MohammedAmeenah 6 ай бұрын

This is the best video have seen. Thank you so much

@SumiKhatun-y9i 6 ай бұрын

Great vedio for cell culture indeed😊

@taylormarkel1094 7 ай бұрын

this is so useful thank you so much for making this!!!!!!!

@minato2363 7 ай бұрын

I guess the falcon tubes can be reused if autoclaved no need to incinerate them, I am saying this with respect to universities protocol no idea why its done in professional labs....

@kosheeka 6 ай бұрын

The best practice is to avoid any type of cross-contamination during the study. Cross-contamination can be expanded to include chemical contamination that may occur if autoclave is not properly carried out. This is a common practice in many laboratories.

@刘涛-c6y 7 ай бұрын

Does this really follow aseptic practice? I doubt that。

@anjalisingh-vq2jv 7 ай бұрын

can you plz tell the concentration of sodium alginate used and also mention the solvent for alginate solution?

@22-traninhquocnam3 4 ай бұрын

Ít’s 1%

@sadiahaquekhan6003 8 ай бұрын

Hi Neil, I am facing contamination continuously in my cultured cell. I checked my media and My insect cell media which i prepared manually using 2x sfc media, gentamycin, P/S ,serum and cell culture graded water. Could you please tell me how can I know that which of any cell culture reagent having contamination or not?? I sprayed alcohol vigorously on the reagent bottles, holders, pipettor, washed my hand, wearing gloves, rubbing my gloves with alcohol, keeping my empty plates and plastic container under the uv inside the hood, still contaminated media... :(

@canceronthebookss4975 6 ай бұрын

You can autoclave the media if you think any component of the media is causing it

@AmruMagdy 8 ай бұрын

@AmMalak-hd9di 8 ай бұрын

Why he put 1.2 instead of 120 ? Someone explain please

@welelameka 8 ай бұрын

Thank you!

@JYOtiRaNJanMANgaRaj 9 ай бұрын

Thank u so much 🙏😊🙏🙏

@AdeelHassan-v1v 9 ай бұрын

extremely helpful

@JosephKyalo-q5t 9 ай бұрын

Very good presentation

@demonwolf6195 9 ай бұрын

Wait why would you wash the cell to remove the medium with the PBS? I mean medium is required for cell growth, why remove it?

@kosheeka 6 ай бұрын

Washing with PBS removes unwanted components from cell cultures (dead cells, debris) and prepares them for further steps (staining, assays) without harming the cells. It provides a clean, consistent environment with the right salt balance for healthy cells.

@ZainAbbas-i3e 10 ай бұрын

wonderful and well presented. hope i could an opportunity to work under your supervision and guidance. i am final year student of biotechnology.

@Zhi-u6j 10 ай бұрын

Very nicely explained sir thank you very much 👍

@thunderstorminmyblood3705 11 ай бұрын

Fantastic, thanks so much!

@maggieweber8419 11 ай бұрын

Is it "safe" to pour cells out of the flask? Especially when working with primary cell lines, would it be better to pipette and avoid the flask's lip? or does it not matter?

@kosheeka 6 ай бұрын

As you pointed out pouring cells out of the flask is generally not recommended. Pipetting is the preferred method for handling cells, especially primary cell lines which are more delicate. Pipetting allows for precision and reduced contamination risk.

@stooteebaruah9678 6 ай бұрын

I had the same concern

@funny11744 Жыл бұрын

very informative and fast. very good technique! Could the counting be made without trypan ? It seems yes ,

@kosheeka 7 ай бұрын

While it's possible to count cells without Trypan Blue, this method doesn't assess cell viability. Trypan Blue helps differentiate between live and dead cells, ensuring you're working with a healthy population.

@mayamanek2182 Жыл бұрын

perfect explanation! simple to understand and the throughoughly produced visuals really helped

@matthewwagner47 Жыл бұрын

Is breathing on it adding potential contaminating?

@kosheeka 6 ай бұрын

Yes, breathing over the cell culture can be a source of contamination. You can introduce bacteria or fungi from your mouth and nose. In a sterile lab, wearing a head cover and surgical mask is essential to minimize this risk. Additionally, working in a biosafety cabinet provides further protection by filtering the air and creating a sterile work zone.

@vocalistguy9850 Жыл бұрын

Absolutely great

@WhatsInAName0 Жыл бұрын

Very helpful 👍

@businessgoose4355 Жыл бұрын

Thank you for sharing your knowledge! Great video

@kimmie2022 Жыл бұрын

Very thorough love this

@b-harmony Жыл бұрын

Thank you this was very helpful!

@aishahashmi2549 Жыл бұрын

Very informative 👍🏻👍🏻👍🏻

@marwatawfik3956 Жыл бұрын

thanks so much

@BlueSkyFight Жыл бұрын

No trypandblue?😮

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@petersamuelemeka8710 Жыл бұрын

Very informative; step by step explanation. This is the perfect video I craved for. Nice work, Cross

@oussboudjelthia4869 Жыл бұрын

Thank you soo much!!!! That was brilliant

@saminathapa829 Жыл бұрын

Very good video , thanks

@ChecedRodgers Жыл бұрын

Excellent and very thorough. A re-upload with louder audio would be fantastic.

@MES-S 2 жыл бұрын

An informative video

@danieloulhint7914 2 жыл бұрын

Mr. Cross, thank you so much for this awsome video. It is really a review and informative for m.. Great presentation and easy to undersand. Kudos from Morocco.

@funny11744 11 ай бұрын

What happens with the phenol red ( from the composition of media): Is the phenol red able to change its color from basic to acidic and then after pH changes from basic to acidic ?

@kosheeka 7 ай бұрын

@@funny11744 Phenol red actually can change its color from basic to acidic and then back again! But under two conditions it will not be a suitable component: 1. Multiple cycles of going from acidic to basic can degrade the phenol red over time, making it less effective as an indicator. 2. If the media becomes very acidic (much lower pH), the phenol red might not change back completely even if the pH is adjusted upwards. This is because the cells themselves might be stressed or dying, causing a permanent shift in the media's pH.

@funny11744 7 ай бұрын

@@kosheeka thank you for information.

@brunadepaula5640 2 жыл бұрын

It was very helpful!! Thanks!

@shahnawaza6710 2 жыл бұрын

Thank you for this nice video

@deepalishenoy6705 2 жыл бұрын

Very nicely explained sir. Thanks a lot..

@ShakespeareCafe 2 жыл бұрын

"Avoid pouring media and reagents directly from bottles or flasks." This is major violation of the sterile technique. Pouring media or PBS introduces aerosolization or spillage of liquids leading to cross contamination or contamination in general. Even though it may seem wasteful, serological pipettes are much cheaper than a ruined culture and loss of cell line.

@greencard1245 2 жыл бұрын

I love your videos! thank you!

@estefanycamilabomfimdossan2308 2 жыл бұрын

This is an amazing work! Thank you so much!!

@funny11744 Жыл бұрын

Question : Regarding the stem cell culture in T 75 flask - using media without CO2 : Can we use a closed cap for T75 flask ?

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