Lisa Sorlie

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@EminorEntertainment 4 ай бұрын

Thanks Lisa!

@isharajwade4610 5 ай бұрын

Fantastic video. Very well explained.. Thanks Lisa! 👏🤩

@azifahahmed4462 5 ай бұрын

Thank you so much

@bidwansekhar 7 ай бұрын

❤

@nohataha6398 Жыл бұрын

what if when using blast for unknown it gives 75 similarity identity?

@corbindavies1089 Жыл бұрын

YES

@veharaariyaratne8440 2 жыл бұрын

This was soooo helpful for an assignment I was stuck in because MEGA kept rejecting the sequences I aligned on ClustalX!!!!!!!!!!!!!!!!!!!!! YOU ARE A LIFE-SAVER HONESTLY THANK YOU SO MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

@monalizapumihic9538 2 жыл бұрын

Thank you so much💗

@seenaagaariiofficial4257 2 жыл бұрын

Dear Lisa Sorlie can you help me in getting the BioEdit software?

@Bigtigerfanmom 2 жыл бұрын

It’s a free download. Best wishes! www.megasoftware.net/

@swaralideshpande3948 2 жыл бұрын

Can we use the same tool for protein

@Bigtigerfanmom 2 жыл бұрын

Try it. I’m not sure.

@swaralideshpande3948 2 жыл бұрын

@@Bigtigerfanmom thank you so much ☺️

@safrajmdraj1976 2 жыл бұрын

Nice 👍

@emilieyoungblood2090 2 жыл бұрын

Thank you so much 🤩

@june5572 3 жыл бұрын

Good video. A few queries: what do the nos 0.001, 0.003 in the clades mean? How can I do bootstrapping? And how do I add the timescale?

@KesshiKyo_shi 3 жыл бұрын

Thank you!

@TheDBZgamer 3 жыл бұрын

ur the best....thank you..and yes i drink milk and read books...

@nemo9396 3 жыл бұрын

How do you identify a gene out of a sequence?

@Bigtigerfanmom 3 жыл бұрын

Look for patterns. Something looks amiss visually.

@joannalynmontemayor3094 3 жыл бұрын

Hello Ms. Lisa,where can I possibly start trimming my allignment from the start and end region.

@Bigtigerfanmom 3 жыл бұрын

You can tell where the pattern starts and ends. Trim the outliers off; usually that happened on both ends.

@MyChannel-nz1tz 3 жыл бұрын

My brothers name is John too.. his last name is Greenler too... wow never met another Greenler before... 🙋‍♀️.. Most come from Defiance Ohio... right?.

@rajatranjan974 3 жыл бұрын

Sir, I am aligning sequencing in MEGA format, but after opening the same file, I am getting message "Align Sequences must be of equal length". How to fix this problem?

@Bigtigerfanmom 3 жыл бұрын

Can you trim the ends? I’ve not seen that before. Otherwise, call a research university in your area- genetics research.

@DameArif 3 жыл бұрын

Dear Dr. I have fasta format and want to convert into ab1 file. Have you any solution of this? Looking forward

@Bigtigerfanmom 3 жыл бұрын

Try these ideas: sequenceconversion.bugaco.com/converter/biology/sequences/fasta_to_abi.php

@babyyoda3655 3 жыл бұрын

Hi Mrs sorlie

@nguyenhuynhnga4635 3 жыл бұрын

Thank you so much.

@leinad9211 3 жыл бұрын

Thanks ms. Lisa! It was very helpful!

@fixfalcon979 3 жыл бұрын

Hi, Thank you very much. Where I can get Mega program??

@Bigtigerfanmom 3 жыл бұрын

www.megasoftware.net/dload_win_gui

@fixfalcon979 3 жыл бұрын

@@Bigtigerfanmom Realy Thank you

@Bigtigerfanmom 3 жыл бұрын

@@fixfalcon979 glad to help

@IMfanclub1 3 жыл бұрын

Tq Lisa for sharing

@morolakeayeyo8525 3 жыл бұрын

hello, the video is really helpful but i am stuck at obtaining phylogenetic tree comparison files. how did you obtain yours

@Bigtigerfanmom 3 жыл бұрын

Morolake Ayeyo google drive is pinned

@Bigtigerfanmom 3 жыл бұрын

It's from University of WI - Madison (research university)

@sihammicrobio3290 3 жыл бұрын

Thanks allot for this greatfull simply vedio , my question is how we can know the code of for example bacteria ? What's the step to do that?

@ladysilat1428 3 жыл бұрын

How we can know what is the accession number? And download sequence of bacteria and make three of alignment? And thank you

@syedfahadshah8461 4 жыл бұрын

How can we contact with u maam

@Bigtigerfanmom 4 жыл бұрын

Laiba Fahad What do you need?

@Bigtigerfanmom 4 жыл бұрын

Actually, I have not taught middle school in years. I think you should contact a local academic university for the most recent information. I am scared I won’t be the help you need.

@syedfahadshah8461 4 жыл бұрын

@@Bigtigerfanmom i am a research student and my task is to construct a phylogenetic tree and then i will ANI it

@syedfahadshah8461 4 жыл бұрын

I think i will need your help

@rabeyatafsire9062 4 жыл бұрын

thank you. But talking about organism information and choosing different options make the video unneccesary lengthy.

@springfield6826 4 жыл бұрын

Drink milk !, read books ! hahahahahah

@mushtaqal-esawy6595 4 жыл бұрын

Really helpful , many thanks Lisa! Can I ask about the percentage or numbers that can be added to the tree image and if I can add a unit to the scale?

@Bigtigerfanmom 4 жыл бұрын

Mushtaq Al-Esawy I’m really unsure of that threshold. I never “maxed out”, but my role has changed from science to library literacies.

@MuhammadHanif-lj8ry 4 жыл бұрын

very nice Dear Madam I accept you as my teacher

@nonkululeko2112 4 жыл бұрын

Hi, that was very informative and life made easy. Please assist, I does not give me the MEGA option (I do have the MEGA v7 software)

@Bigtigerfanmom 4 жыл бұрын

Magagula Nkululeko B I would have no idea. I would call your nearest research academic university.

@retrocoin1001 4 жыл бұрын

Thank you Lisa this video is easy to follow and all steps are clear.

@sarahpopple6297 4 жыл бұрын

Even with a more recent version of MEGA this tutorial still worked perfectly! Thank you :)

@santoshbisht8788 4 жыл бұрын

Hello mam can you provide me your emal. id?

@Bigtigerfanmom 4 жыл бұрын

SANTOSH What for?

@santoshbisht8788 4 жыл бұрын

@@Bigtigerfanmom Mam i have started my phd and having some objectives of DNA barcoding so need your help in analysis and bioinformatics too

@Bigtigerfanmom 4 жыл бұрын

SANTOSH Hi. I forwarded this request to someone that may be able to help you.

@santoshbisht8788 4 жыл бұрын

@@Bigtigerfanmom thank you so much mam

@sydneythekidney8484 4 жыл бұрын

How can you download audiobooks?

@Bigtigerfanmom 4 жыл бұрын

Sydney Grover the directions audible-uk.custhelp.com/app/answers/detail/a_id/5481/~/how-can-i-use-my-ipod-to-listen-to-audible-audiobooks%3F-%28mac%29

@sydneythekidney8484 4 жыл бұрын

@@Bigtigerfanmom THx so much :) I really didn't think you'd reply because the video was like 3 years ago lol. Thx again!

@jonnyboy0203 4 жыл бұрын

Hi! I'm trying to convert .txt files to .fasta files and am seeming really struggling! I am wanting to form a phylogentic tree of multiple species across 200kb. I've been trying to find a method of doing this for hours and cannot find how to do it!

@Bigtigerfanmom 4 жыл бұрын

Jonny Shaw Did you check out the link in below comments?

@bellebooth1741 3 жыл бұрын

@@Bigtigerfanmom I’m struggling with this same issue. Not sure what link you are referring to also. Could you paste it here?

@sebooz4949 4 жыл бұрын

why did you put an accession number to find K. lactis from the database? is it a reference strain? Do we have to put reference strain when constructing a phylogenetic tree? Thanks

@Bigtigerfanmom 4 жыл бұрын

sebo oz Technology probably has changed since I made this tutorial. I have not worked with genetics actively for sometime now, as my role at work changed. You probably don’t have to have an accession any longer. I’m pretty sure, though, we did at the time.

@sebooz4949 4 жыл бұрын

@@Bigtigerfanmom thanks, so sequencing the data is enough I think. Without reference strain

@Azrael928 4 жыл бұрын

Nice video, but could you teach how I can change or delete letter in an abi or scf file?

@Bigtigerfanmom 4 жыл бұрын

jorgensen.biology.utah.edu/wayned/ape/ This was recommended because it’s free and accessible across multiple platforms.

@cassiopeiahagnazata7580 4 жыл бұрын

hello! where can i get ab1 files?

@Bigtigerfanmom 4 жыл бұрын

drive.google.com/drive/folders/1N8EcmMmua6J3loMoEr5JBX7PhTSI29pf?usp=sharing Please check under "Lisa's Samples." Thanks much!

@cassiopeiahagnazata7580 4 жыл бұрын

Lisa Sorlie omg thank you so much! is there a sample for staphylococcus aureus?

@Bigtigerfanmom 4 жыл бұрын

Cassiopeiah Agnazata I don’t think so; I haven’t been teaching this class for a few years. I’ll ask UW Madison if they can help you out.

@cassiopeiahagnazata7580 4 жыл бұрын

Lisa Sorlie thank you so much! 💙

@Bigtigerfanmom 4 жыл бұрын

Here's a Google Drive with some examples to explore: drive.google.com/open?id=1N8EcmMmua6J3loMoEr5JBX7PhTSI29pf

@MJ-ye7dd 3 жыл бұрын

Thanks so much Lisa.

@sheilayong6371 3 жыл бұрын

Hi, may I ask why I click insert sequence from file, and I used the sample from the google drive (U94922 misson file), its not working.. it says the mega cant read the sequence

@Bigtigerfanmom 3 жыл бұрын

Do you get an error message? What does it say?

@jaliyljones5351 4 жыл бұрын

Hi. So i had to go and get each homolog fasta for the HTT gene. But now when I export the alignment and head to do my phylogenetic tree, it won’t let me select what i just exported. Any suggestions?

@Bigtigerfanmom 4 жыл бұрын

It's got to be related to the type of file, in my opinion, that you're uploading. Please check the extension of the file and see if the software you're using accepts that.

@Bigtigerfanmom 4 жыл бұрын

Was the file cleaned up (cropped where it gets messy) before the export? Also, maybe the Bioinformatics-2 by JSBiology tutorial may help?!?

@Bigtigerfanmom 4 жыл бұрын

Also, go to 5:36 on my tutorial and rewatch the segment following that time. I wonder if that is the problem.

@MichaelWitbrock 4 жыл бұрын

Of course, you or I will not have the pre-loaded phylogentic tree file with the 5 other kinds of Kluyveromyces partial ribosomal genes waiting to be added in "edit". And they are extremely hard to search for in NCBI. (the Accession numbers in the screenshot aren't very helpful. BUT the GI numbers like 31581082, 4038945 etc (at the left of the species ID in MEGA) are. So, tediously search for each of those, send it to a FASTA file, and insert each of those files into MEGA. Sigh. Also, the automated open in MEGA on download thing seems to be a Mac "feature" and super dangerous. If on another kind of computer, just insert the file in edit mode.

@Bigtigerfanmom 4 жыл бұрын

Michael Witbrock This tutorial was made for middle school students. I’m teaching in a different capacity now. Do you need files? What can I help you with?

@MichaelWitbrock 4 жыл бұрын

@@Bigtigerfanmom Hi - don't need anything. It was useful. However, there is a point about 5:30 seconds in, where you load a file PhylogeneticTreeComparison.fas, which most of the 96K viewers of the video won't have -- I constructed a version of the file myself, after working out that it wasn't a standard demo file provided with MEGA. Perhaps you could add a link to a video where you show the watchers how to construct that file, by using the BI codes to find the other 5 genomes on NCBI. Fantastic that you're making this resource for intermediate school students, but maybe with a couple of tweaks it could be useful to even more people. All the best

@Bigtigerfanmom 4 жыл бұрын

@@MichaelWitbrock I think I have the file on an old Apple computer (back in the day when I could afford that type of computer). I'll check for it and post it in the notes after work today.

@MichaelWitbrock 4 жыл бұрын

@@Bigtigerfanmom That would be fantastic. I hope you found it!

@Bigtigerfanmom 4 жыл бұрын

Michael drive.google.com/drive/mobile/folders/1N8EcmMmua6J3loMoEr5JBX7PhTSI29pf?usp=drive_open