How to cite this?

may i know lymphopre same as lymphoprep?

How much is the maximum speed of the centrifuge in rpm or g?

Excellent presentation

Human Cell Atlas 💙

Thank you for the discussion.

Thanks!

Great informational video even for a dummy surgeon! The transcriptome description as the interplay is what I have wondered about for more than 30 yrs! thank you

I experienced this Cytokine storm during COVID. Its an extreme form of a asthma. Imagine you breath like you breath through a tetrapack straw. I got temporary relief with the vicks inhaler and asthma inhaler. Since nobody helped except my Mom a retired doctor found ivermectin. After some difficulty acquiring the drug i got relief within the day with hyper sence of smell and taste. Imagine police searching a room while looking for a suspect and firing without looking. Thats how the storm works the immune system knew there is a virus or germ but it cant find it so its atacking everything.

Hi thank you so much for your kind video! is IP lysis buffer and IP buffer the same? and why do we need IP elution fraction at last? thank you in advance.

thank you so much for your knowledge

The narrative does not match the video. It’s not a 1:1 ratio of blood to PBS; from what I observe, they use at least three volumes of PBS to dilute the blood. Correct me if I'm wrong.

You break concepts down so well!

Thank you. I have an exam in 2 hrs and you are doing gods work

Thanks for putting together this information..i could see many people's efforts in this

Are there any method can alter fluorescence stainning method? Cause my lab doesn't have fluorescence microscope

A very informative overview of immunotherapy advances

Kindly share the link for part i and ii

Hello! The DAPI was already in the mounting media?

what is the box used for PBS rinsing called?

what is d full meaning of PBS? from d video?

What this video exposes, with all of the references to the alphabet soup of immune cell components nomenclatures and the complex world of cell markers, is the still desperate need to adopt a completely new methodology toward curing cancer. The current conventional techniques of targetted therapies are flagrantly ineffective, this is obvious from the statistics of cancer deaths even with the advances of CHECKPOINT INHIBITORY IMMUNOTHERAPY or other cell specific targetted chemotherapy drugs because our medical community still does not properly understand GOD'S great design of life and the way in which all of our machinery works. We need a more SYSTEMIC or generalized methodology which does not rely on deciphering or affecting the very elaborate and complex world of individual cell markers but which destroys and targets all cancer cells no matter where they manifest themselves...an approach which identifies and targets some sort of UNIVERSAL property, some common vulnerability or weakness of all cancer cells which does not require compromising our own immune system. One such promising possibility is simple OXYGEN THERAPY which is based on the idea that no cancer cell can survive in an oxygen rich environment. All cancer cells are cells which have been controverted and have become unable to metabolize energy by oxygenation or aerobically and instead do it anaerobically by FERMENTATION and are therefore vulnerable to OXYGEN exposure. This type of OXYGEN THERAPY has been written about in thousands of scientific articles and has proven to be effective and which has been implemented by various doctors in various ways. These include the oral hydrogen peroxide therapy of DR. DAVID WILLIAMS, the intravenous hydrogen peroxide therapy of DR. ROBERT ROWAN and the sodium bicarbonate therapy of DR. TULIO SIMONCINI...all variations on the same theme and all of which have a great track record of success...and yet all ignored by the mainstream medical community probably because there is no money in it. Now I have also recently learned of specific case histories and success in curing stage four cancers using HYPERBARIC OXYGEN THERAPY...another variation of this same method but all still ignored by the majority of the mainstream medical and pharmaceutical communities because, again they are virtually FREE and do not generate huge profits...UNFORGIVABLE In conclusion, we in the general public should implore our medical professionals to more seriously explore the potential of this totally non intrusive, non toxic and systemic OXYGEN THERAPY because the WHO statistics have proven that the number of deaths from cancer worldwide has INCREASED in the last fifteen years despite all of these so called immunotherapy and drug specific advances...deaths have INCREASED not DECREASED !!! LET US THEREFORE TRY SOMETHING ELSE.

This music makes me feel happy about the process :)

this was 3 years ago. What has been found and the current treatment for C19 causing cytokine storm in the lungs.

Awesome info

Including ALS

where can someone get this therapy? My brother has state 4 metastatic colon/rectal/met to lung. Please help

Superb. SUBSCRIBED!

Thanks for this. So glad I'm still in the control group.

Considering how detailed and informative the poster is, and how useful a walk-through could be, presenting it as a 360-degree panoramic video is spectacularly hopeless. Even at max YT resolution most of the text is too pixelated to read. Someone went to a lot of diligent effort on this, but it doesn't work.

Thank you So much

The Qognit application isn't allowing me to input anything int he "PE (Reporter Channel)" and "APC (Bead Channel)", since nothing pops up in the drop down menus and no text that is input in those drop down menus is saved. What to do?

Excuse me, in the first step, is there any centrifugal removal of plasma in the whole blood before PBMC separation?

Can this be used to collect both the plasma and mononuclear cells, or will the reagent disturb the contents of the plasma?

I can't wait till they start work on CAR-T cell therapy for Lupus.

Sorry, I was disappointed with this video. Wrong expectations, I guess. I hoped to see something that goes into the weeds, like effects of using one type of column or another for purification, manipulating Fc region, conjugation methods on the product's performance. Watching suits chat about antibodies was not it.

Indian docs are GEM ❤️💎💎

Is it possible to have cytokine storm after asymptomatic Covid 3 months before?

8:42

Could this "inflammation be triggered by the vaccines..?

Excellent ... well done ... does silent inflammation in the Gut via Microglia influence Apoplexie ?

Role of TNF alph in corona ?

can you go from 100% alcohol to Xylen without letting slides dry from alcohol first? also why he was dipping slides and not leave them there for 5min?

Can we incubate FITC conjugated antibody in the Co2 incubator

How much ficoll is need to add.

No link to part 2?

Thank You.

Thank you very much.

I appreciate your comprehensive and short training

It would be good to have a walk through of using the infinity flow package from raw data to UMAP.