Slide Repair and Coverslip Removal
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Пікірлер
@amelkhalfallah6134
@amelkhalfallah6134 Жыл бұрын
If the slide cover before stain what to do?
@emmanuelernest46
@emmanuelernest46 Жыл бұрын
On my way to histology exam thanks
@EmilioCafferataChea
@EmilioCafferataChea Жыл бұрын
Thank you very much for this useful video. May I ask you how much time do you leave the slides in acetone, and xylene for the removal?
@gineesh78
@gineesh78 2 жыл бұрын
Thanku so much
@yanliu309
@yanliu309 2 жыл бұрын
Nice video. Very helpful! Could you please share detail information of the warmer your were using at that time. Thank you!
@jenniferlinxm
@jenniferlinxm 3 жыл бұрын
Does your H&E slides lose some of the colour during the process of removing the film coverslips through acetone, absolute alcohol, and xylene? How do you minimize that lose?
@Arraymold
@Arraymold 2 жыл бұрын
There is a minimal loss of color. Just don’t leave the slide in acetone longer than a few minutes.
@qorryamanda
@qorryamanda 3 жыл бұрын
Hi. Thankyou for your very useful video. I'm currently working on a atherosclerosis induced project in murine models and have the difficulty in staining the tissue with old oil red o powder based solution in our lab. I intend to remove the coverslips mounted by aqua based mount media and reapply the oil red o staining kit from another manufacture. Do you have any suggestions on how I can possibly remove the coverslips mounted with aqua mount media without damaging the tissue on the slide? I was going to go for the xylene / xylol alcohol method but afraid the xylene will wash out any remaining fat or foam cells i had in the slide. Do you have any suggestion? Thankyou.
@michaelbailey9643
@michaelbailey9643 3 жыл бұрын
Ah, KZbin, you deliver once again! Needed a rough idea of what a paraffin block punch looks like for a customer call. Thanks!
@davidw.9711
@davidw.9711 3 жыл бұрын
Hi just bought this array mold (3 mm size) and just gave it a try. So far it worked fine!
@histopath3989
@histopath3989 4 жыл бұрын
Thanks for the detailed instruction. It is very helpful. I encountered a problem when I was building a frozen tissue microarray by using the kit. I used the mold to build a base with 2mm holes and punched 2mm frozen tissue. But somehow the frozen tissue is larger than the hole and can't be pushed into the hole. Is here any solution for this? It looks from you video that the liver tissue is soft, is that why it is easy to be pushed into the hole? Thank you.
@Arraymold
@Arraymold 4 жыл бұрын
The tissue is frozen. But I believe my needle from being in and out of the cryostat warmed up so the tissue punch core inside in the needle thawed slightly. If you use needles other than the ones we recommend you can have smaller or larger core sizes. If you do have questions please email Arraymold. We try and answer all questions regarding tissue microarray construction.
@rosaria1056
@rosaria1056 5 жыл бұрын
Dear Thom Jesen, I'd like to say that your work is outstanding. Best regards from Brazil.
@aamirbashir720
@aamirbashir720 6 жыл бұрын
Nice
@joannedayan9922
@joannedayan9922 6 жыл бұрын
Hello. I am new at Histopath practice. Just started two weeks ago. Your video made my day... Thank you. :-)