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Пікірлер
@paulnovak833 6 күн бұрын
Ive never read a protocol that uses charcoal. I love that youve brought this to my attention. Is the paper or source i can learn more about the process? Thanks
@IggyJReilly1980 8 күн бұрын
Whatever happened with this experiment?
@cactifanatici 6 күн бұрын
@@IggyJReilly1980 very slow grower but I still have it growing in tissue culture and optimizing the media each subculture
@IggyJReilly1980 6 күн бұрын
@@cactifanatici Would you share the hormones and amounts in your latest media? My present plan is to use 1.0 mg/L BAP and 0.5 mg/L IAA, MS, PPM, Sucrose, Agar.
@marcellinofresquez4213 14 күн бұрын
I love this lol!
@doolittle6977 15 күн бұрын
do u prefer charcoal in your media?
@cactifanatici 15 күн бұрын
@@doolittle6977 yes always
@KeithKreider 18 күн бұрын
Media composition?
@cactifanatici 17 күн бұрын
@@KeithKreider full details on my patreon but it’s MS based
@andrewbob3002 18 күн бұрын
Unfortunately I sold off my flow hood years ago. how practical would it be to use a SAB to propagate cactus cuttings? The wash looks like it would be problematic with a SAB.
@cactifanatici 17 күн бұрын
@@andrewbob3002 no problem at all
@dragonsimon1 18 күн бұрын
What does the charcoal in your media do to help your cultures?
@cactifanatici 17 күн бұрын
@@dragonsimon1 helps with toxin accumulation
@chetmanley2462 19 күн бұрын
How much faster are those bioreactors w/ liquid media as opposed to gel culture media?
@cactifanatici 19 күн бұрын
@@chetmanley2462 depends on the purpose. They are very fast for multiplying but other stages better done in agar
@jordanbarrs2185 28 күн бұрын
Thank you so much for your videos
@jacobgarcia3597 29 күн бұрын
hey man, what lights are you using on your arios? are those lights sufficient for them to bloom?? im curious because i want to make a set up just like yours. thanks
@miztoya3646 Ай бұрын
I have some sweet potato callus that I would like to turn into shoot, any advice?
@chetmanley2462 Ай бұрын
How old
@BearMeat4Dinner Ай бұрын
Walio . Thanks! I am a new subscriber!!!❤
@michaelmurray214 Ай бұрын
I found this channel from the plants in jars channel. What I'm most interested in is TC in the Euphorbia species. Do you know if anyone is doing this?
@cactifanatici Ай бұрын
@@michaelmurray214 I’ve only done euphorbia obesa but I know some others that have been done in academic papers. I think the latex is annoying to deal with probably lol
@cactifanatici Ай бұрын
This graft failed lol😂 No update!! Pereskiopsis is a much better option and auxccrss with TC grafts
@HoangHuy-vh2gp 2 ай бұрын
can you tell me about tissue culture anubias nana petite what dosage of medium is needed i am trying many times but all failed, the plant does not sprout and grow with: Ms: 2.4g Kinetine: 0.15g Agar: 4g sugar: 15g look forward to your feedback
@cactifanatici Ай бұрын
@@HoangHuy-vh2gp is that using a full media recipe? Have you tried MS with Vitamins 30g sugar. No hormones. Or just 1 mg BAP is where I’d start
@HoangHuy-vh2gp Ай бұрын
@@cactifanatici no kinetin just MS, sugar and agar right you?
@cactifanatici Ай бұрын
@@HoangHuy-vh2gp MS with vitamins but yea
@drakeweddner 2 ай бұрын
Did yiu ever do s update
@rhysezfpv 2 ай бұрын
Is this the one that clumps ? Is it also psychoactive ?
@yibofan6855 2 ай бұрын
Thank you for posting this. It seems like an open flame that torches the containers' brims was unnecessary and it's okay to hover over the explants. I was taught with these cautions in school but I want to improve my efficiency by simplifying the process. This video is informative.
@Primitivemycology 2 ай бұрын
Microwaves are a great way to have non sterile media and a low success rate, buy a pressure cooker for more consistent results. The microwave will work for a beginner that is not yet serious about this craft.
@HappinessForUsTFC 2 ай бұрын
This looks so fun! Kudos brother, I'm going to follow your path and try this when I get my laminar flow cabinet. Thanks for sharing. Those boxes look great to use.
@ChipsandIcecream37 2 ай бұрын
super glad to see you posting again !!!!
@Akashic_Monk 2 ай бұрын
Fire 🔥
@mcactus6210 2 ай бұрын
Wow seem amazing
@leevermaak9028 2 ай бұрын
Looking fire how old are they
@jacobg147 2 ай бұрын
Do the successful tissue cultures have a similar growth rate to new seedlings?
@cactifanatici 2 ай бұрын
@@jacobg147 faster if in optimal conditions
@CannaGro81 2 ай бұрын
Thanks for tje knowledge 🙏
@CannaGro81 2 ай бұрын
Awesome. I'm just getting into Lophophora
@shawnbutcher1153 2 ай бұрын
Damn the things I would do for a flow good😭
@TheRootedPepper.Slugwell 3 ай бұрын
@cactiFanatic do you have a list of hormones and where to get them. Such as a package deal where I can buy them all? I’m not tech savy so I don’t really know much out online stuff. Took me forever to find this video again. And another couple days to figure out how to comment on KZbin. Do you have an email?
@cactifanatici 3 ай бұрын
Yeah email me at [email protected] My patreon has that list and info
@sailanabila4605 4 ай бұрын
How long will the cactus grow in this media?
@cactifanatici 3 ай бұрын
As long as it needs to. I usually transfer every 1-3 months as needed but can stay forever
@nickygrobler2979 4 ай бұрын
Could you lease send a link for your patreon account
@cactifanatici 4 ай бұрын
www.patreon.com/Cactifanatici?
@monicabasson7936 4 ай бұрын
What tissue culture media do you use
@cactifanatici 4 ай бұрын
I specify specifically on my patreon recipes but it’s MS based
@machankinson1795 4 ай бұрын
Best Asmr ever
@Mycoheterotroph 4 ай бұрын
Do you use PGRs in your media? I’m about to start a large tissue culture operation of palo santo but definitely want to branch out into xerophiles and carnivores, and for the palo santo I will be trying somatic embryogenesis and from there using a bunch of pgrs to pump shoots, etc
@cactifanatici 4 ай бұрын
Sometimes on my patreon I go more into the hormones and when I’m using them
@Mycoheterotroph 4 ай бұрын
🙏🏼🙏🏼
@escapeRC8 5 ай бұрын
Sir how about auxin and cytokinin how many milliliter or ppm per liter
@cactifanatici 5 ай бұрын
To anyone wondering I’ve already explained this is a horrible graft and did not work. First and last time. San Pedro and pereskioposis are my go to stocks with almost 100
@jerryribyat1481 5 ай бұрын
Why did you hack the hell out of your grafting stock go back to grafting 101
@cactifanatici 5 ай бұрын
Yeah lol …. This video is old. First and last time I ever did this
@joeysurge4069 5 ай бұрын
what is media recipe u use for callus and seeds
@josephwilliamcosta 5 ай бұрын
Do you have a video or pictures of the results of this?
@cactifanatici 5 ай бұрын
Death lol
@dragonsimon1 5 ай бұрын
Have you found these to be efficacious for most of the plants you work with?
@cactifanatici 5 ай бұрын
Yes TIB systems al has do better but the crop can be lost easier also
@dragonsimon1 5 ай бұрын
@@cactifanatici How so? You mean contamination rates are higher?
@cactifanatici 5 ай бұрын
It’s easier to spread the contam yes
@Frankiemind 5 ай бұрын
Can you use agar to start the from seeds as well?
@dutchvonderlinde1693 5 ай бұрын
RIP to the rootstock and scion, there is no way this worked lmao
@cactifanatici 5 ай бұрын
Facts lol!!!!
@dutchvonderlinde1693 5 ай бұрын
how do you germinate seeds on this type of media? can you just put them on it and wait and leave it in the cup for a while? do you have a vid' about it ? cheers
@Alproverse 6 ай бұрын
Thank you for sharing this🤝🏽
@brandonbernard4171 6 ай бұрын
Awesome videos. Thanks for just being straight up and not dressing it up like most other videos on the topic
@philippheidel-weizel1385 6 ай бұрын
Nice work, keep it up!