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@Nabil-s6b
@Nabil-s6b 9 ай бұрын
great explanation
@samahbouhdjeur8820
@samahbouhdjeur8820 2 жыл бұрын
thank you
@Ghostly_whizard
@Ghostly_whizard 2 жыл бұрын
Very well explained . thank you !
@domatech
@domatech 2 жыл бұрын
Excellent explainations
@ahmetc.
@ahmetc. 2 жыл бұрын
Hey man, thanks a lot. Very very very clear:)
@marlonrettis5009
@marlonrettis5009 3 жыл бұрын
Great explanation of HILIC. Thank you!
@is-3_beetle906
@is-3_beetle906 3 жыл бұрын
Thank you very much for such a good video!
@goodthingscomeinpairs6833
@goodthingscomeinpairs6833 3 жыл бұрын
Thanks for this video 🙏
@shodexsingapore4869
@shodexsingapore4869 3 жыл бұрын
Reversed phase or RP chromatography is most popular in HPLC field, BUT it uses Hydrophobic interaction. So it is not compatible for Hydrophilic substance like Sugar analysis, such as Mono, di, tri-saccharides and Sugar alcohol. This video shows the applications of Shodex Asahipak NH2P-50 4E can help you to analyse hydrophilic substances with HILIC. 0:00:00​ Getting started 0:00:35​ Anomer separation 0:01:01​ Avoid anomer separation trouble with high temp 0:01:30​ Avoid anomer separation trouble with alkaline condition 0:02:13​ Sugar analysis 0:02:49​ Mono, di, tri saccharides analysis 0:04:08​ Oligosaccharides analysis 0:05:15​ Vitamins, taurine, inositol, stevia analysis 0:05:43​ Summary
@shodexsingapore4869
@shodexsingapore4869 3 жыл бұрын
Wondering how to analyze under alkaline condition? 0:00:00​ Getting started 0:00:34​ What is RP chromatography? 0:01:00​ Theory of RP 0:02:29​ Polymer vs Silica column 0:04:14​ Example of alkaline tolerance 0:05:06​ Azithromycin analysis 0:06:16​ Fat soluble vitamins analysis 0:06:29​ Summary
@shodexsingapore4869
@shodexsingapore4869 3 жыл бұрын
HPLC column deterioration could be caused by void volume generated inside the column. This webinar excerpt shows a tips on how to avoid generating void volume. 0:00:00​ Getting started 0:00:18​ Void volume due to high pressure 0:00:31​ Void volume due to spike of column pressure 0:00:42​ Case study 1 0:01:21​ Case study 2 0:02:35​ How to prevent void volume
@shodexsingapore4869
@shodexsingapore4869 3 жыл бұрын
Reversed phase or RP chromatography is most popular in HPLC field, BUT it uses Hydrophobic interaction. So it is not compatible for Hydrophilic substance like Sugar analysis, such as Mono, di, tri-saccharides and Sugar alcohol. This video shows how samples are separated under HILIC condition. 0:00:00​ Getting started 0:00:40​ Theory of HILIC mode 0:03:20​ How samples interact with packing material 0:04:19​ When to choose HILIC mode? 0:05:09​ HILIC vs Reverse Phase 0:05:42​ Summary
@shodexsingapore4869
@shodexsingapore4869 3 жыл бұрын
What is inside Shodex HPLC Column Box? How to know if your column is in good performance for your coming analysis? 0:00:00​ Getting started 0:00:07​ Confirm product code 0:00:20​ Remove tape and open carton case 0:00:43​ Read COA and manual carefully 0:01:40​ Make sure column is in good condition 0:02:19​ Conduct COA test
@shrikantpawade2755
@shrikantpawade2755 3 жыл бұрын
Very nicely explained. Thank you
@kishoremane1592
@kishoremane1592 3 жыл бұрын
Unable to understand your Pronounsation
@shodexsingapore4869
@shodexsingapore4869 3 жыл бұрын
Thank you for your feedback. We hope the English subtitles in the video can help you understand the content better. Cheers,
@marlonrettis5009
@marlonrettis5009 3 жыл бұрын
"Pronounsation"? LOL!