Спасибо за интересное видео жду новых выпусков

For Serum protein separation? Whts G typ made of

G-200?

Why do u stack the plates after pouring

Bro why's your autoclaved pipette tips OUTSIDE of the hood lol. What's the point of the hood then

2 micro liter of the?????? what?

What is with the comments section here lol

Sir, You did lots of mistakes in whole pricess, these are as follows: No lab coat, no face mask, no sterilized hand, no autoclave Petrydish, no flame treatment before and after (open- shut) of the media, no suitable conical flask according to media size, pouring skill also not expert etc. If some body not satisfied please contact me at my WhatsApp no +919984078615 (India)

Dammm!!! I thought this was a link to a geographical location about tectonic plates & earthquakes..(how, Ahh dunno) Alex,..Alexxx...what mushrooms did you put in this sauce!?? What tis disss? Ehhh

Lol, he just put his dirty finger on the inside of the petri dish...

I don't really understand sir.

I know this experiment

Sephadex G75 is used in........ ?

Sir vary nice video sir

hi

Use flame sir

Please use sterilized petry plate

I was just wondering, is it better to use disposable, sterelized plastic petri dish or reusable, glass petri dish? Thanks.

Thanks good solution for lb agar plate technic

“Ay-gar”.... an “auger” is a drilling type of device. While it is true that some obscure places do list “auger” as an acceptable pronunciation there is no reason to use the one possible pronunciation that has alternate meanings.

what material is sephadex made of?

wheres ur lab coat -_-

Hi. I want to sintetize de Oligo dt(12-18) and Random Primers (d6)n and save some money. How do I have to configure de requirement (order) for the primer syntesis lab? I know that the 5' of the oligo dt must be modified wiht a PO3, but that's all I know lol... Please, help me

Thank you very much for all your videos, I watched them all. There are some things I would like to express to new students. 1) ALWAYS wear gloves 2) When you stick your hands out of a biosafety cabinet use a disinfectant before you put your hands back in. 3) Never touch your face, your jeans etc. with your gloved hands and continue to your work!! If you did, change gloves. 4) Work in the middle part of the cabinet. Dont come close to the opening(where you put your hands in) with your samples. He almost took the petries out while spreading which is a contamination risk. And dont go far back since you will block the air circulation. 5) Never leave the lids of the pipette tips open. 6) The opening of the cabinet should not be in line with your face. Especially if you have to talk. You may contaminate things inside and some spills may come up to your face (true story). 7)Whenever you need to put something inside the cabinet, first spray disinfectant to every outer part of it. NEVER directly take stuff and put in a hood. Even if it is a GMP facility with clean rooms.

something definitely went wrong... its everywhere

hi dats gudoooooo

Your flask is way too big. Should have used a smaller one

why no more videos? :/

you are not on you PPE while doing the exercise

What Sephadex (G10, G25 ...) you are using? What g you use for centrifugation?

Why would you use a spreader vs an inoculation loop?

going a little further were special booths to cover your shoes.

Using the proper techniques as we see in the video allow us to have good non contaminated agar plates for later use, and thank you for all your lab skills advice. It also is recommended to wear lab coat, mask for your mouth and nose and glasses. Roberto Pizano

Hey just want to ask does agar utilize the water in the media for solidification i.e is it a water based polymer?.I prepared LA with varying PEG conc.(0,5,10,15,20,25 & 30% w/v) PEG is known to decrease the water activity creating a water stress at 40 degree celcius agar solidifies around at that temp.Only 0 & 5 % solidified the rest did not forming some sort rubbery white disc pellet.The agar that i used is simple bacteriological agar for growth studies.

try 1L bottles sir, you don't get any bubbles if the pouring rings are fresh. with this method you waste your time.

Thanks for posting this

Easy to understand.

Do I need a BSL1 or BSL 2 for the cloning step?