can you make tutorial for Ubuntu and doing analysis from command lines like doing blast, trimming seq, etc?

@58:44 This plot allows you to see if there is a subset of your sequences which has universally low scores. Y axis: Total number of reads. X axis: Average Quality Score

I have a transcriptome file which I want to do this analysis but I don't have a reference genome...so I have to de novo assembly...can you please guide how to do that?

great explaination Thank you

Sir one of the best video so far i have seen very detailed explanation of rna seq work flow and how to use galaxy

This is one of the best videos I've seen on RNA and ATAC Seq. Please can you share your email or linkedin with me? I have some questions and follow up. Thanks.

This is beautifully explained. Please make more videos on related topics!

Hello, I am trying to do analysis on RNA seq data obtained on E. coli. But when I run String Tie or htseq count, I am getting errors such as An error occurred with this dataset:format tabular database ? or An error occurred with this dataset: format gtf database ? I am using gtf file downloaded form NCBi, I am unable to resolve this. please suggest what can be done in order to complete the analysis

Dear Dr. Singh, I am concerned about your breathing pattern. Please see a doctor. I feel you are running out of breath too soon while talking. Also gasping sometimes, I mean no disrespect. I am genuinely concerned. Great tutorial tho. I love your work. It would be wonderful to accompany you in your research if there is any position !

:)

Very useful and interesting tutorial, Thanks a lot!

Sir, thank you so much. easy to understand about such a difficult contents.

I'm starting this today and I will update it very month to let y'all know how much progress I have made. !!

Great! Very informative and explained in an easily understandable way

Hello Sir, I am learning NGS, thank you very much your videos are really helpful... I have a question, can we upload the reference genome while using Hisat2 for mapping? Please suggest

What do one do if you do not have a transcriptome file, a gtf.gz-file?

Can I use Galaxy software for viral NGS data??

how to get dataset that you have been use here ?

Very nice and helpful tutorial, thanks sir!

Thank you so much, sir

At 1:24:28, I didn't find any zebrafish genome in the reference genome to run HISAT2. Can I upload it from history?

Thanks!! I'm trying to understand Galaxy, your videos (3) are really helpful! (Y)

Thanks!! I'm trying to understand Galaxy, your videos (3) are really helpful! (Y)

what if the data is too big to reach 2gb, what to do? because to achieve metagenomics will fail

Can’t thank you enough

WinSCP could be used instead of filezilla or cyberduck?

Thanks a lot. This is very useful to me to learn Galaxy

Great points! Wish I could use Galaxy for exoma analysis! Still learning about it.

It is a very useful video. I have been learning to use the Galaxy Webserver lately. This makes it so much easier and helpful for me. Thank you

Thanks. All seven sessions are helpful.

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Great! Thanks for the efforts!!

Great presentation. Thanks for your efforts.

👍. Great initiative.

V.nice Presentation 👍👍👍