What do we use to sterilise the mortar and pestle between each sample? Is 70 % ethanol okay for this purpose?
@ihateeveryone23173 ай бұрын
hi
@allendorcoodo93053 ай бұрын
Which one of your dna kits is best for soil(if any)
@ildk1013 ай бұрын
hi, thanks for the useful tutorial and explanation. I have a question: Are the components His-tagged? I would like to have my protein with His tag and that would make purification complicated.
@gobalkrishnan.v5 ай бұрын
is possible to dna and rna simulation as realistic. and what kind change in human body, if done modification them?
@19jaig7 ай бұрын
Congrats! always a reference in molecular biology and genetics! We get your NEB products here in Mexico and use your great online tools! Keep up the good work!!
@ForensicNA8 ай бұрын
how do I actually know the microliter required for the 5-10 unit, say if I have a 10,000 unit/mL RSAI
@drtariq37928 ай бұрын
Hi mam! I have a problem I extracted the plasmid and did digestion for many time Always got no band after digestion or sometimes not properly digestion What is the possible reason behind this I did this many time all times failed
@drtariq37928 ай бұрын
Hi, I extracted the plasmid and ran the gel and got good Bands of plasmid but I did not get band after restriction enzyme , did not properly digested my plasmid What is the possible reason behind this? Please guide me I did it many times
@JennyMiranda-Villasanti11 ай бұрын
how would I go about simulating a restriction digest when I have my plasmid sequence in one file and my DNA insert sequence in another file? Would I just paste the sequences one after the other?
@antoinesalmond9442 Жыл бұрын
❤
@PooleySteen Жыл бұрын
If someone at your laboratory deliberately left 5 mL of T7 exonulease gene 6 out at room temperature for one hour what would said to them?
@nebiolabs Жыл бұрын
We’re sorry to hear about your enzyme! At NEB, we always like to assume benign intent, so perhaps your lab mate was checking whether your stock of the enzyme was heat labile? We recommend reaching out to the enzyme manufacturer’s Technical Support team to see if they can help!
@PooleySteen Жыл бұрын
@@nebiolabs No just a fifty five year old acting like a child, because he didn't like a new protocol. As for contacting technical support they would be some biochemical wizards if they could put it back together, but more then happy to receive 11,000 dollars for new stock.
@lofithemequranslowandreverb. Жыл бұрын
Can you share the Notes to get knowledge of Gibson Cloning
@JohnGross-n1v Жыл бұрын
Every time I try to do this, a loading icon shows up and disables me from doing everything. I've heard others say the same, this doesn't work.
@MoonMm-zr4uf Жыл бұрын
Thank u sir it is very helpful!!!!!!!
@aech5170 Жыл бұрын
great video, very well explained and easy to understand :>
@zalavijay9675 Жыл бұрын
thank you sir
@flaviabarrosasoares3047 Жыл бұрын
I love it
@detectiveboy9190 Жыл бұрын
Cell free protein means protein synthesis in a test tube????
@非典型国漫放映暨 Жыл бұрын
could be done long time ago
@buitienchuyen88 Жыл бұрын
Ai cho xin tên bài nhạc này dc k nhạc hay
@hazmatrec Жыл бұрын
i come here every pi day. hi chris!
@franklyspencer4181 Жыл бұрын
I'll never forget how you cured me permanently from my herpes virus with your herbal supplements. You're a God sent #DRERAYO 🌸🌸
@franklyspencer4181 Жыл бұрын
I'll never forget how you cured me permanently from my herpes virus with your herbal supplements. You're a God sent #DRERAYO 🌸🌸
@julianj86092 жыл бұрын
How far does the exonuclease chew the DNA? How does it know when to stop?
@njomza00 Жыл бұрын
Up to 50 bp
@fezilemkhontfo66152 жыл бұрын
I need help with my experiment please help, I am trying to add enzyme to the PLA and observe the degradation
@nebiolabs Жыл бұрын
Please email our tech support team at [email protected], they would be happy to help.
@hizkiacorinthians2682 жыл бұрын
Thanks a million
@ridingpatriot2 жыл бұрын
Well this aged well..get tested as much as possible!
@AHG6812 жыл бұрын
How many beads are used for blood collection in glass bead tube?
@nebiolabs2 жыл бұрын
What glass tubes are you using?”
@Sajtia2 жыл бұрын
Is it 100ng per microliter or is it in the total volume?
@sallausaidu22522 жыл бұрын
What is the implication of preserving kidney tissue at -20 degrees Celcius, if there is any?
@acesin-et7pp2 жыл бұрын
The Gibson assembly does not faithfully join linear fragments. It needs a backbone vector to in the master mix. If only insert fragments are used it will also chop the outward 5' to 3' sequences from both strands, resulting in a single but truncated PCR product whose ends are shorter.
@Oscar-ns9wg2 жыл бұрын
aeugh
@WakeUpItsAbuse2 жыл бұрын
Hi, thank you for your focus on Mansonella infections. Do you offer testing in the US? It's been several years since this video was posted- have you added testing for Mansonella Streptocerca or the new clad of M. Perstans DEUX, and if not, do you plan to ?? Do you currently offer testing to consumers or only to medical professionals? Thanks!
@vitoarifin85602 жыл бұрын
Sip wes
@MrReeceyburger1232 жыл бұрын
Crazy stuff.
@ElEmokWearsPrada2 жыл бұрын
Is it necessary use the loop primers?
@filippacubillo972 жыл бұрын
p̲r̲o̲m̲o̲s̲m̲ 😭
@osmosislifescience99602 жыл бұрын
My Life revolves around CRISPR Thanks for the video
@edwardzuo24142 жыл бұрын
that smile killed me..
@lewisatff66002 жыл бұрын
video anda sangat bagus dan mempunyai mesej yang luas terima kasih
@marioscleaner2 жыл бұрын
Hello, does this also apply to the role of DNA ligase in DNA replication?
@hendrikelliott75582 жыл бұрын
😄 ƤRO𝓂O𝕤ᗰ
@gayathris49382 жыл бұрын
This was so informative...great video!!
@nomercy59432 жыл бұрын
You can choose for one of the above gifts
@raulipauli42042 жыл бұрын
Hjjjjkgggg
@jennymacparry18553 жыл бұрын
Whole genome PCR and mini-primer PCR Also make a video on these 2 topics . Thank you.
@bjjvh65333 жыл бұрын
The video sound is pretty good, beyond my imagination