Hi Bidipta, as you know, nowadays biology is highly interdisciplinary in nature. A research project is almost never pure "ecology", "evolution", or "cell biology." So if the panel asks you how to approach a research question, they will expect you to think about and discuss different techniques and model systems (flies/mice/yeast). So the answer is yes, they may ask you questions related to other disciplines if they are relevant. Hope that helps. All the best!

Interesting. Do post the link to the paper, would love to look at it and update the video.

Most welcome!

Thank you!

Thanks! You'd need at least 150 uL for a 1 mm path length cuvette. Regarding protein concentration, 0.2-0.5 mg/mL is a good start. However, it may vary depending on the secondary structure content. You can start with a concentrated protein solution and dilute accordingly. Check out the following links for more details: 1. www.ncbi.nlm.nih.gov/pmc/articles/PMC2728378/ 2. cmi.hms.harvard.edu/files/cmi/files/cmi_cd_getting_started.pdf 3. structbio.vanderbilt.edu/wetlab/cd.sample.prep.php

@@SpartanTutorials Thanks a lot! Truly appreciate your help.

There is no wrong answer here. Just be open and tell them what's true. If you have applied to other institutes, tell the interview panel about them and your reasons for applying to them. This will help the interviewers in knowing more about your research interests.

@@SpartanTutorials thankyou sir

Thanks Nitin

Thank you so much for the kind words! Glad to know you liked the video!

Good question! We only label the 5' end because we want to be able to distinguish the different bands produced by DNase I digestion and match them in the order of their sequence. As I explained in the video, we also run a sequencing gel to know exactly where the protein binds. If we had labeled both ends, we would get a mixture of fragments from both ends and we would not be able to sort them.

@@SpartanTutorials then why don’t we label the 3’ end only

@@cart2795 We can, but then the sequence will be read backwards. Why make life difficult? 😀

Thank you! Glad you found it helpful.

Thank you. Glad you found it helpful.

Thank you!

Most welcome

Thank you!

Indeed!

Sad but absolutely true!

Sorry to hear. Please consult your family physician, they''ll determine the best course of treatment. Wishing your son a speedy recovery.

Glad you found the video helpful.

Glad you liked it, Josie. Do let me know if there is a concept you would like to see explained.

Hi Suraj, haan main series mein poori immunology cover karunga. Thank you for watching, and do let me know if you have any questions.

Hi Mark, Great to know your good experience with the drug and nice to see improvement with all the useful markers - weight, blood glucose, and HbA1c. I think your lifestyle also contributed a lot.

How did you get the drug ?

Thank you so much Sir! Hope they find them useful.

Thank you!

Thank you!

Thank you!

Good question. No, phenol-chloroform is specifically need to remove all the associated proteins. Ethanol is used for removal of salts and precipitation. So both reagents are necessary.

Thank you!

Thank you!

Thanks!

Glad you found it helpful.

Good question! GLP-1 and Glucagon are highly similar in terms of their amino acid sequence. See this explanation from a paper: "The mammalian proglucagon gene (Gcg) encodes three glucagon like sequences, glucagon, glucagon-like peptide-1 (GLP-1), and glucagon-like peptide-2 that are of similar length and share sequence similarity." I think it is likely that there was gene duplication in the course of evolution that gave rise to these highly similar peptides, which have specialized to evolve slightly different metabolic roles.

Hi Harsh, Glad to know it was helpful.

Hi Gouri, sure, noted. Will make it soon!

Glad to know it helped; Best wishes!

Thank you for the pointer!