Abnova

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@beinghuman4457 3 күн бұрын

can i use this precedure for total RNA extraction from brain tissue

@dr.hannibal8338 10 күн бұрын

why we used DMSO?

@Bijaya-fe4vp 16 күн бұрын

After centrifugation with isopropanol, I get 2 layers? Is it ok

@user-sm4sf4ff2i 17 күн бұрын

Cheer~~~determine the content or quality of (a metal or ore).

@youraffirmations2458 21 күн бұрын

The video is 13 years old, protocol has updated since then. Those days this was the method. Today the protocol is modified so follow the latest procedures. Overall, the basic principle will always remain same. ✌

@edwinabichedid9616 25 күн бұрын

song name?

@lumieredice485 29 күн бұрын

6:27 What is that purple solution?

@priyankakalkutagi1901 Ай бұрын

Sir Why use parafilms only ?

@user-hg2sh5lv8b Ай бұрын

خب بابا میدونیم میدونید بیدارم زنگ زدن نمیخواهد که

@vinnied7 Ай бұрын

Your thumbs are contaminating the sample.

@alexandreluizborgessilva6018 Ай бұрын

to fix the antigen, can I replace the paraformaldehyde with formaldehyde?

@renassalahudden9520 Ай бұрын

what is the name of this lab please? i want to apply for PhD studies they looks so amazing

@funny11744 Ай бұрын

I am interested in finding out if the smallest surface ( that with volume signs) of the flask is treated for cell culture.

@Nancyyoutube 2 ай бұрын

Thank you for your informative video. I was wondering if 1mM PMSF is added to 10 ml of RIPA lysis buffer. It would be great if you could clarify the exact amount of RIPA lysis buffer.

@nareshbarik5384 2 ай бұрын

Thank you 🙏

@joshj747 2 ай бұрын

Song is a banger a 2x speed

@leewilliam3417 2 ай бұрын

Great lesson😊

@christopherchong3899 2 ай бұрын

Reading the protocol - the volume of Trizol needs to be titrated according to the volume of cells to be obtained, and whether the cells have been grown in monolayer or suspension. Trizol is quite powerful so can degrade the cells and RNA altogether. Don't use 1 mL Trizol uniformly - only the volume you need. The volume of Trizol affects the volume of Chloroform, Isopropanol and Ethanol to be added later in the reaction.

@fatcammal 2 ай бұрын

this is stupid and convoluted find a way to do this in one pot. dont waste my time

@spectroxis6418 2 ай бұрын

Taking my baby steps into research, your videos have helped a lot!

@aondohembanege9551 2 ай бұрын

Hi, thanks for the video. Please, I have a question: I will like to ask a question about cell treatment experiment with a test drug for 72 h. In such an experiment, do I have to add my drug once and incubate for 72h then check the cell viability or I have to remove the drug solution and replace with the same drug and concentration after each 24h to avoid wrong readings due to degraded drug in wells or evaporation? Please any other person reading the comments can also reply me if you have the correct answer to my question. Thank you

@user-dn9lu2gz6k 2 ай бұрын

Could you please explain the steps of rehydration ( what you used and how much time in each bath) thank you .

@user-ij2zg7gn4l 2 ай бұрын

can you tell me about cell line name? i really want to know that..

@SuWen-ju1zg 2 ай бұрын

Very nice

@music_bit8516 3 ай бұрын

Таке собі

@leonardolopez2849 3 ай бұрын

Good music

@zeinabrahnama5749 4 ай бұрын

Soo helpful thankss

@anyarudko6308 4 ай бұрын

What happens if you incubate at room temperature the primary antibody?

@haryordeleholuyemi7351 4 ай бұрын

nice analyses

@mechkitten 5 ай бұрын

I appreciate the video warning against late behavioral/social interventions in the "Wait and See" method. By then, it is a game of catch up of addressing side effects while trying to get to the root cause. Giving extra support may provide different mileage, but withholding has much more drastic implications.

@djahidaachouri2504 5 ай бұрын

Please tell me if the sample could be a plasma or a serum from patients aimed at the determination of their protein or antibodies concentrations ?

@jujetaly322 5 ай бұрын

Wats the pink solution that is removed at the beginning of the video?

@tenzinglobsang8587 3 ай бұрын

The culture media most probably

@user-ds9pc6fx1d 5 ай бұрын

Fantastic

@odilijasafinaite9021 5 ай бұрын

Hello, I have two questions 1. After collecting my sample and adding Trizol, can i store it in -20C for a month? 2. Why we must change centrifugation 12000g to 7500g in etoh wash step? Can i perform this step with 12000g?

@farshidardabili5101 5 ай бұрын

Why the upper phase is red, phenol-chloroform phase and the lower phase is aqueous ?

@47kudalesaurabh17 6 ай бұрын

the website that you have provided is not working / not found for optimization of IEF program

@microbiologist177 6 ай бұрын

y this is not working with DNA fasta file

@aliexpressdonotbuyproductx4346 6 ай бұрын

Does the T25 flask has the cap without holes when it arrived ? If yes could be used for cell culture especially if we loose the cap as you probably did before injcubation?

@hans6550 6 ай бұрын

can i do my mtt not inside the bisafety cabinet?

@aishwaryalaxmi2173 6 ай бұрын

Great 👍

@sulemankhanvirologist5015 6 ай бұрын

Very interesting thanks for your kind explanation ❤

@funny11744 7 ай бұрын

A question- Why the Keratinocyte Growth Medium is a serum-free medium? What the serum could harm stem cell or human in case of intravenous treatment? thanks.