11.17.22 Cell Cycle Analysis with Jack

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[@nikitaluthfiadriyana2695]

Thank you your video help me a lot!

[@FlowJoMedia]

Glad to hear that!

[@_clarisse_manishimwe]

Hi Jack, thank you for the video. My axis is linear but I still can't calculate the model. Any idea on how I can solve that?

[@FlowJoMedia]

Hi user, After setting the axis to linear, draw an initial gate around the G1 peak. Then set constraints to generate the rest of the model. I almost always draw a gate on the G1 peak, then set G2 = G1x2 and set the G2 CV = G1 CV. The G2 peak should theoretically have about twice the intensity of the G1 peak (though sometimes it has slightly less). The CV of the G1 and G2 peaks should theoretically be the same. If you need further assistance please reach out at [e-mail]!

[@job506]

Hi Jack, Many thanks for this video. I got above 100% in my cell cycle statistics. In addition, I have negative percentages too. What could be wrong?

[@FlowJoMedia]

Hello, please reach out to [e-mail] and we will be happy to assist you with this issue!

[@s12360131]

Hi, Thanks a lot, but I still have a question. Why the CV of the G1 and G2 peaks should be the same?