A Day in the Life of a Motor Protein

[@WayneT85]

sounds like john needs a raise

[@tortilla6960]

True

[@Bigapplegirl81]

😂😂😂

[@idioticlight]

I dont think he gets paid at all

[@jokestermasteroflaughter7754]

I can’t afford to pay , ya know corporate cutbacks and all

[@KlaxontheImpailr]

And a union

[@RoboThot711]

The Johns in charge of delivering seratonin in my brain must have gotten lost

[@ryn.999]

Serena Koehler Yo... same

[@gandhipragash1443]

@@ryn.999 but serena will help it...

[@rageisblue979]

bro, my tears just left my eyes while ı am reading this

[@AtarahDerek]

There was a major accident on their usual roadways, and the detours set up for them are a lot less efficient for their commute.

[@fortheloveofallthingsholy2706]

well it seems you need to change aspects of your life to fulfill that need. a mental attitude change will be light and day for you.

[@positiveoutlook5557]

This is what I've been waiting for. Explaining biology through analogies and cartoons. Now all that's left is for someone to create a video game of sorts where you pass through the body as some molecule or protein.

[@LuisAldamiz]

You would not have much fun because proteins have no volition whatsoever: it's all determined for them, they are machines. No choice-making, no fun.

[@sevensciencequestionsandwh9145]

Yeah. One guy built a space shuttle in his back yard after watching several animated episodes of Superman.

[@LuisAldamiz]

Sergio Ezquerro - Sounds both cool and very challenging, but for what I know most videogame designers tend to simplify, because more complexity = more work = more processing power load = more lag and problems of all kind, etc. Ask Extra Credits, let's hear what they have to say about that.

[@Turambar3791]

You are really late into this I think, then: kzbin.info/www/bejne/sHLZfJ-cjapniZo

[@miri8851]

You could play that ancestors the humankind odyssey

[@al35mm]

This is a very good, fun video. What a lot of people probably don't know, is that these motor proteins do actually walk. They have two legs and walk in a similar way to, um, how John walks only thousands of times faster. They are also in all of your cells and not just in brain cells. Great fun stuff!

[@ScientistCat]

Yeh! what I loved most about this animation, his legs moved like the actual thing.

[@BeauxLo]

Nowadays I can’t tell if it’s sarcasm or a joke

[@cabbage4994]

@@BeauxLo it’s neither, in fact it’s true

[@Tazzycat432]

Kinda adorable sounding

[@hyhyd6135]

So basically Motor Proteins are like Sonic the Hedgehog

[@MellifluousLies]

What an incredibly well-made video. This helped me see past the complicated jargon of vesicle transport. Science and art should always be friends.

[@shybound7571]

john is the one person in the group project who does 99 percent of the work

[@Chakravarti2911]

103% in my opinion. Dynein and Myosin are adding -3% work.

[@annamyob]

@@Chakravarti2911 OMG LOL yes. I just attended a meeting yesterday with Dynein and Myosin busily contributing their negative three percent!

[@Piglin_Emperor]

John being so Kind to Carry the Other Ones When the First thing they do when they wake Up is Make him a Problem: I am *THE* Embodiement of Kindness

[@Vitammortem2380]

So true

[@BertieW0oster]

My biology teacher never told us that motor proteins have eyes and wear slippers! I can't wait to tell him something he doesn't know! :D

[@Lolzwutz1]

Pink and fuzzy, mind you

[@Alinask84ever16]

Victrola Fix whahahhahaha. You're damn funny! LOL

[@legojoker7552]

wtf is a motor protein? I only know a John

[@megamushroom]

Victrola Fix ГOГ

[@米空軍パイロット]

@@megamushroom GOG

[@tatkins1943]

Excellent... it is time for Pixar to grab these characters and begin to describe life at the cellular level!!!!

[@jamestheotherone742]

yeah... no. Hollywood and Pixar's idea of scientifically accurate is "Inside Out".

[@imEden0]

@@jamestheotherone742 nah, they specifically went for a non physical intepretation

[@MooMooMath]

Very creative and fun to watch. Go motor protein go

[@galaxymind7595]

Random Guy what

[@galaxymind7595]

Random Guy how

[@Frission_imaging]

Cringe

[@darrenanimatic9675]

John lives in a neuron.

[@lewis9s]

The John’s carrying my brain cells during exams must really have it hard, huh.

[@carlosvoices966]

Next Disney movie: cell

[@sarahwalter9465]

Osmosis Jones

[@tahiranjum4610]

Why there is not one already?

[@Alex-Skimo]

def would watch

[@raffimolero64]

inside out: realistic mode

[@dewfall56]

Yeah Disney is starving for new ideas.

[@039stephen]

john works so hard to keep you alive and you just sit here watching youtube videos.

[@TheDutchPhysicist]

For those curious, the city is utrecht in the netherlands

[@AndyHage]

I was, thanks

[@SukacitaYeremia]

Then what does the bell tower represents? (lol, jk, no need to answer)

[@dam11232]

No it's not. It's a cell

[@luxfux8764]

4:46 „University of Utrecht“

[@Loudencerstudios]

⁠John lives in the Netherlands

[@vinijoncrafts2882]

4:30 I love how when he hears "braind disease" he's like "oh hell nah" and starts walking faster lol

[@gigglysamentz2021]

Oh my god this is absolutely amazing ! This video is so well made and explains very complex processes with excellent analogies ! I'm a biochemist and knew these motor proteins, yet I still learned a lot.

[@wellesmorgado4797]

Very cool! Even John's humble steps are by themselves marvels of physics, chemistry & engineering. Hoping you do a video on them too.

[@aryaa3998]

wow, this is amazing! John has nice purple slippers

[@faizrafii58]

and annoying way to walk

[@mohamedmagdy621]

after this terrific explanation ...u just cared about slippers !!!!! damn

[@HOTD108_]

​@@mohamedmagdy621Hey Mohammed, lighten up.

[@ivymarimo1631]

Haawhqkjq

[@ivymarimo1631]

​@@HOTD108_the mohamed must chill brou

[@spathiphyl]

i just discovered this video almost 11 years after it has been posted , this is GENIUS

[@taravanderveer7842]

THIS IS AWESOME! I teach biology and the students tend to not have an appreciation for the cytoskeleton and its importance. What a great video to help them appreciate how cool these guys are. Thanks!

[@Topfen77]

Check out kurzgesagt then! It has tons of illustrated stuff about biology, science, and physics! Also why we should terraform Venus instead of mars.

[@bulletsizednuke1100]

Bruh, in high school the cytokskeleton was only a footnote

[@leonardoguglielmi4032]

to everyone who made this, thank you. this is the best ever made cell biology video i've ever seen.

[@travismaenle9416]

the human body blows my fucking mind. complicated shit is happening in order for me to post this comment. and you to read it.

[@scirp6039]

watch your language.

[@travismaenle9416]

no

[@eggur0ll289]

Dylan Branch he or she can say anything they want u don't like it? Get off the intetwebs bruh. U know how much cussing there is in middle school? High school? Even online gaming. Grow up

[@kerstinramoka6063]

Travis Maenle agreed

[@AMRINDER-123]

How can someone watch language, it does not have any state.

[@promitchakraborty]

Awesome animation and extremely well thought out and executed, rendering a complex topic easily understandable . A delight to watch. :)

[@andrewd1763]

fun video, but since kinesin can only move toward the plus end, how does john get home?

[@CSissi95]

Andrew Dominguez through dyenin which can only travel in the opposite direction. without each other, they'd remain stuck at one end of the microtubule

[@andrewd1763]

Mind posting a sources? Would like to read this

[@CSissi95]

Andrew Dominguez I found this which explains it a little bit, but I had a class this semester about the cell biology and metabolism which included a good component on motor proteins such as kinesins and dyneins. I thought it was really interesting and definitely worth learning about! www.ncbi.nlm.nih.gov/books/NBK21710/

[@andrewd1763]

Also taking a cell bio course. Assuming dyneins and kinesins are both bound to a membrane. How are the ATP binding site's globular head domains inhibited to allow movement in one direction or the other?

[@CSissi95]

Andrew Dominguez we didn't cover the part explicitly explaining why one goes in one direction whereas the other goes the opposite. I'm assuming the shape of the head domain can only bind in one direction to the tubulin dimers which make up the microtubules due to each molecule's dispersion of charges and overall shape. The dimers all assemble in a specific configuration if you look up the structure of microtubules in a picture, so perhaps the motor proteins' head domains bing their front edge to say the alpha-tubulin while the back end of the head domain binds the beta-tubulin or vice-versa? Again, we didn't cover this part so I can only hypothesize.

[@Shadowmech88]

The way John walks reminds me of the animation I've seen used for motor proteins in other videos. The similarity across multiple depictions makes me wonder - do we actually know the proteins move in that specific way? Have they been observed making those movements under a microscope or something?

[@scrambledmandible]

I can't say how they found it, but yeah that's how motor proteins move

[@qdaniele97]

They look somewhat like pairs of shoes walking along wire/chains. Obviouly, what is actually happening is that the "feet" interact chemically with the sections of the microtubule alternatively attaching and detaching from it, but still is funny to think of them as tini tiny pairs of shoes walking around.

[@Shadowmech88]

@@qdaniele97 Do we know that because they have literally been watched moving that way in real time via microscope, though? Or is it a guess based on their structure or other information we know about them?

[@bettievw]

@@Shadowmech88 as far as I know they have in fact been observed. I believe by an electron microscope if I recall correctly. I hope someone else responds who knows for sure, I'll look it up and tell you if I find out.

[@dogwithacoolhat]

@@Shadowmech88 scientists usually run simulations of how proteins would fold which then can get depicted visually. Proteins are far too small to be seen to my knowledge.

[@markus19999]

People who didn't believe me that there was a voice inside my head need to see this. It was John all along!

[@ConnectSparrows]

What an absolutely phenomenal video. Thank you guys for creating this and helping a learning student understand the mechanisms of motor protein function. The city analogy, bringing it to a bigger scale, was helpful!

[@Schregger]

Man, John puts up with this shit everyday... what a trooper.

[@alisonlaett9625]

Thank you for publishing this stuff online, it's amazing how much free educational content is available today!

[@annamyob]

The macro world where we are educated by a 'free' platform funded by advertisers hoping we'll be snagged by their clickbait, is much weirder than John in his pink fuzzies and his buddies!

[@creation966]

My classmates laughed their asses off when our prof played this video in class! #UBC #BIOL200

[@perceivingacting]

But who is telling John where to go?!?! :) Is there a video of what's inside John's brain? Coordination is everything!!!

[@budbundy8028]

Except for what has been explained in the video, John is on a one way street.

[@LuisAldamiz]

Electric polarity of the microtubules. John is a molecular machine obeying chemical laws, the overall machinery of the cell is still amazing.

[@wingsofpurityofficial4031]

@@LuisAldamiz Sorry for necroposting, but I thought I would mention that the polarity of microtubules is not electric but instead based on which direction the microtubule is assembled in. The plus end of the microtubule is the direction that it is produced in, and the minus side is the part of the microtubule attached to the centromere (the structure on which microtubule production occurs.) It is kind of difficult to visualize, so here is a video: kzbin.info/www/bejne/iJPFpZWqZ85rrqM As for the basis of John's direction of movement, it is based on the asymmetrical shape of tubulin, the protein that microtubules are made from. The subunits of kinesin (John) will only bind to a tubulin molecule if they have a particular orientation. It is similar to how sticking a 3-pointed plug in an electrical outlet only works if you push it in the correct way. The orientation at which they bind causes kinesin to point towards the + end.

[@LuisAldamiz]

@@wingsofpurityofficial4031 - Not bothered by "necroposting" myself, as long as the info is relevant. So you're saying that polarity is only "accidental" and does not itself determines how the kinesin moves? OK, I take notice, thanks. Always cool to learn.

[@wingsofpurityofficial4031]

@@LuisAldamiz The polarity is not accidental. The microtubules actually start being made at distinct structures in the cell called microtubule organizing centers. And it does determine how kinesin moves, but not in a very direct way. Kinesin cannot determine which side of the microtubule is which, but it can determine which direction a single tubulin molecule is pointing. Since all tubulins are oriented the same way in a microtubule, kinesin can indirectly recognize which direction to move in by only binding to tubilin molecules that are pointed in a specific direction.

[@samdawson7560]

These characters, if I saw them in real life on the same scale, would instill nothing less than existential horror. Thanks for the science lesson.

OMG, this video is so much fun! It was very good for me to watch it after studying the cytoskeleton, it is going to help me remember the proteins and their functions. Thank you guys for the video, and thanks John and all motor proteins for keeping up the good work! :D

[@OXIR]

What an amazingly well done movie. Props to everyone that worked on this video. I wish there were more short movies like this. Makes the topic much more interesting and easy to learn and also reveals new ways of thinking about it.

[@fzigunov]

What a gem. Thanks for teaching us something about neurotransmitters! We really appreciate the effort put into this production.

[@rosegarvey4546]

I absolutely love this video. I have referred back to it many times throughout my Biology university degree to refresh my memory on motor proteins. Very well done, and much appreciated!

[@smooooth_]

John... You walk weird

[@VueiyVisarelli]

+Smooooth Just a lil' motor protein swaggah, lol.

[@jackkraken3888]

That's actually how the motor protein actually walks, and it has nothing to do with being a gay or anything of the sort.

[@karanpatel4583]

Can't believe shits are walking in my brain

[@laurel5432]

That's the best part about this video, because of how accurate the walking is

[@WiseandVegan]

John lives in a city called Donald Trump, that's why.

[@PeliHamsteri]

I just love the fact that you have literally some little dudes (like motor proteins & other cells) working on your body, going through difficult things to keep you alive.

[@zerocalvin]

i love the internet, today i learn biochemistry without taking a course in a university...

[@rundor8]

And what use will it do you without a degree?

[@zerocalvin]

well, like most thing i know, it doesnt do me any good, but it doesnt mean knowing it, is a waste of time. for example, if my kids happen to ask "why do we eat?", i can go deeper then "we eat because our body need the energy"

[@DogeDELUX]

***** Excellent reply, rundor just got burned :)

[@yomommasofat5296]

+DOGE™ wtf doge ur everywhere

[@DogeDELUX]

Yomomma Sofat omg I'm so famous :O

[@hollygolightly8048]

My favorite YT video. I watch it at least once a week. It made a very complex mechanism easy to understand. Animation was brilliantly executed.

[@TheTangi29]

Amazing work ! I'm studying medecine in France, and this videos helped me to understand the global working of kinésine with MT ! Thanks a lot, you saved 1 hour of my precious time ! lang leve de Nederland

[@hiddendrifts]

watching this feels like a fever dream at time, seriously...

[@donross7820]

What fabulous graphics! I love this incredibly creative teaching style. Thank you

[@totalrecone]

What an extraordinarily awesome production! This show taught me what 3 years in lecture theatres couldn't.

[@imnotabeetleiswear6549]

Can you imagine waking up and heading to work, only to be cut off by a massive Motor Protein walking in one direction?

[@BookofYAH777]

you should make a series for kids out of this. This will probably be the best kids series ever made.

[@NaeemAkramAndroidiOSApp]

I'm falling in love with John

[@gustavocoimbravieira963]

his body is so sexy

[@MouseGoat]

John is love, John is life... and I mean that 100% literally, he literally is what make tose to tings possible

[@johnk3903]

I'm flattered

[@madscientistshusta]

Hands off skank!

[@reversehappy9210]

Don't get any ideas *Cough* -fanfictions- *Cough*

[@allfunnydogsstories2129]

2024: my attempts to find similar awesome videos like this are in vain 😢

[@jaslyn5869]

Very adorable (: It really helped me in visualizing and remembering. Please do more of these and help science students like me. Thank you (: Appreciate John more now :')

[@Topical_Solution]

Bro dropped the hardest Motor Protein video and thought we wouldnt notice

[@pfever]

Thank you John for your hard work! It is greatly appreciated 👍

[@NathanHarrison7]

Genius. All learning should be this fun and easy. Subscribed.

[@chaotickreg7024]

3:00 This song is called "Push Pull" by Eskmo and I'm going insane recognizing the niche experimental music I listen to at age 14 coming up in a biochemistry video.

[@moochinarayan7146]

So had an exam, was supposed to write about motor protein, started answering by "so john wake up.......

[@Khloe_dancer_model]

😆😆👏🏻❤️❤️

[@ThatFellowOnline]

This is fantastic! Exactly how to make science accessible to people!

[@wallrider4194]

Special shout out to John the kinesin motor protein for carrying the vesicle while facing painfully difficult challenges.

[@mariarilou6977]

That was amazing! Well done honestly thats what we need at our schools. Creativity!

[@empmachine]

I love the detail in John's steps, totally has the twitch down!

[@dennyhamrick2552]

Something not mentioned is that motor proteins can generally only move in one direction. Once they get where they’re going, they get torn apart and their pieces recycled. Sorry John!

[@annamyob]

Oh sad. Portraying motor proteins as animated persons has a bit of a drawback there. I am shifting in my mind to seeing John as a Lego bot, for whom disassembly and reassembly is a natural function! (Edit: but I do still appreciate the fun animation)

[@Remhad]

Okay the tug of war segment was awesome 👏

[@syedarjimand3593]

I hope John is doing well. Loved your video❤

[@1248-f8u]

This is the easiest to understand way to explain this concept I’ve seen. Very nice.

[@joshgiesbrecht]

Really good animation. Very enjoyable. I have a question though that wasn't really answered, what's the point of John's "friends"? The ones that bind to alley ways and try to walk in the opposite direction. It seems their only job is to make it hard on poor John. What other uses do they have? They seem absolutely useless other than to slow him down

[@joshgiesbrecht]

OK I understand dynein now, he helps John get home, and vice versa. But myosin, what does it do?

[@lenatina22]

+Josh Giesbrecht what does dynein do? Or how does he do that?

[@joshgiesbrecht]

+amina ali I'm limited in this field, but my understanding is dynein is essentially "turned off" while the motor cell goes to its desired location. Once it is there, I believe chemical reactions or instructions "wake up" dynein so that it can continue its journey back to its home. Like I said, I have very limited knowledge in this field. But that is my understanding.

[@StevenMDiLauro]

+Josh Giesbrecht I've just seen this post, so sorry if you've found an answer already! If John walks along the main roads (microtubules) that run lengthwise along the axon from point A to point B inside of the cell, you can think of the myosin as (basically) 'wanting' to walk along the alleys or side streets (actin filaments) to the cell's surface.

[@christophergreen5735]

+Josh Giesbrecht The other two proteins seem to allow the protein package to be passed into other cells, iirc.

[@amraaji9158]

The best video I've ever watched on youtube

[@wjgthatsit2357]

bowling alley screens when you get a strike

[@gabedarrett1301]

If science was taught like this, I guarantee you that more students would care. And that's coming from a student who loves science

[@kayechiu5200]

Best science video for studying I've ever watched!

[@sanchitpriyadarshi257]

My goodness. All my goodness dissolved it's that unexpectedly good. Pure quality work.

[@christophergudgeon9902]

science is so cool.

[@friendlyanalyst273]

+Christopher Gudgeon ikr! ^^

[@MajorasWrath1]

@Yahawah is God False.

[@dot4464]

@Yahawah is God why would god bother making it so complex..

[@dot4464]

@Yahawah is God wouldn't it be more impressive if the body worked magically without all these little molecules controlling it..

[@Jonathan-ob2fk]

@Juno Donat I dont know much about what you say. But I can say definitiely your last sentence is wrong! It is historically proven that Jesus did exist! There were just too many witnesses that saw him! And there are even historical people who saw Jesus after his crucifixion, so after he was risen.

[@divalyri6735]

I absolutely LOVED this video. And I loved John's purple fuzzy slippers. Great explanation!!! Thanks.

[@ManuelArciniega]

I love Science.

[@tosanesoko726]

Me too!!!!!!!!

[@JBoyle-jr9wb]

Yeah i know, this is like nerd comedy

[@PuppetGorillaTag]

Absolutely legendary.

[@FchGuepardo]

Claping my hands !!! Good video !!!

[@thetokutickler]

I half expected John to get hit by a giant bowling ball at the end, and the screen to say "STRIKE"

[@MrChildren87]

I dig the use of Boards of Canada in this clip.

[@owleyes551]

@Pendejoto666 Pretty sure it's from "Tomorrow's Harvest", I forget which track

[@SpelKille]

@@owleyes551 Split Your Infinities

[@rock3tcatU233]

John is the MVP. 💪💪

[@MrBlancify]

Godspeed John.

[@youkofoxy]

base in reality surreal visuals with metaphoric narrative combine with actual science. I like it.

[@NelsonBrown]

I thought the street scenes looked like Utrecht! John better keep alert for bicycles!

[@FishNABucket]

Thank you John for all your hard work!

[@SteveMcRae]

Wouldn't it have made more sense to have named him "Sam"? :) Wait for it....it will make sense in a second.

[@OlemVolle]

Still waiting

[@johnadams-wp2yb]

Sam protein?

[@SteveMcRae]

Sam Kinison

[@OlemVolle]

Yeah I am really eager to find out what this is all about, but I still don't get the reference. A more thorough explanation would be required for it all to come together, I think.

[@SteveMcRae]

It's a kinesin....and Sam Kenisen was a famous American comedian. (the names sound almost exactly the same)

[@elmerfudd5650]

It's incredible enough that it has a mission to take the package to it's destination, but, it's even more astounding of how the packages are made, and for what, and where are the going and why?

[@saadzafar3774]

EXCELLENT EXCELLENT EXCELLENT EXCELLENT EXCELLENT EXCELLENT

[@rosemimi973]

This is the cutest and most informative video that I have seen in a long time.

[@DarkWolf22k]

2:55 I came here for this and you should too... I need the full song NOW... Push Pull Push Pull~

[@venuuuxs]

its push pull by eskmo

[@FIRE.FOX-FF]

Thankyou very much John😁😁

[@mirajtawa1190]

after "john" delivers his cargo , what happens to him? does he get fired ( no further function) , die (degraded) or will he go back and get more cargo to bring (re used)?

[@95johndeering]

+miraj tawa They chop him up and eat him

[@Ghorda9]

he travels on the back of another protein going the other way.

[@nmakarowski]

@@95johndeering thats terrible he has a wife and kids to support

[@Khloe_dancer_model]

Nicole Makarowski Lol! 😆👏🏻❤️

[@sciencehubbyanitamaam4775]

I love the explanation. Very easy to understand. I wish every topic should be explained like this one

[@azu319]

Hello, is it possible for you to allow for translation? I want to show the video to my middle schoolers and I want to translate it to spanish

[@Echoesoftimelover]

Azucena González Wish you were my teacher. All we did was paint stuff and remember their Spanish names.

[@PirateTHESteam1]

No hablo darling. this is american content. by americans. for americans.

[@Echoesoftimelover]

PirateTHESteam1 Asuming she's not in North or South America because then she too would be "American." Oops

[@Echoesoftimelover]

PirateTHESteam1 Triggered loser, you can see yourself out.

[@so1zy]

This video was made by a university in the Netherlands.

[@christophercharles9645]

Thank you for your service, John!

[@jrlmenezes1]

This comes to show that Utrecht has the best pot in Netherland :-)

[@spacemanspiff6332]

For a guy who wanders around in house slippers John puts in work.

[@AwesomeBrony]

am i the only one who recognized that one song from boards of Canada in 2:07?

[@cleankilljack3271]

+Mallinda name please?

[@AwesomeBrony]

Boards of Canada - Split your infinities

[@cleankilljack3271]

Mallinda Thanks

[@AwesomeBrony]

+CleanKillJACK no problem! :)

[@KushTs]

+Mallinda +CleanKillJACK do any of you know what's the song that starts at 2:51? :s

[@leyspun]

This was the most surreal part of my childhood, thanks

[@Zheartbreaker]

who transport cargo wearing slippers anyway

[@ayebraine]

because it looks like slippers in the 3D model of actual thing: watch?v=B_zD3NxSsD8

[@TonyHaku]

Best and easy explanation with real life experience. Love it,hope will make easy to understand for biology students

[@andvokslife9596]

Great thanks and glory to the Creator, for His wisdom!

[@gfag776]

kek

[@faysalaboveaverage]

John: A man of focus, commitment and sheer fu_king will!

[@SimonsAstronomy]

We need to thank john for his work 🫡

[@K4inan]

But wait... how fast is the protein actually?

[@cvs8104]

200 mph , about how fast your able to react to things

[@K4inan]

Kristoff sund Damn, it's moving those slippers very quickly then.

[@cvs8104]

lol im kidding its moving at the same rate your blood could shoot out of your veins

[@K4inan]

Kristoff sund Right...

[@cvs8104]

lol im kidding again i wouldn't know

[@hp8644]

Het is echt ongelooflijk hoe mensen dit kunnen zien en vervolgens denken dat dit stukje design uit de lucht is komen vallen. Met name de wetenschappers zelf!