AAV Purification Using AEX and Mixed-Mode Chromatography

Recombinant adeno‐associated viruses (rAAV) are among the most promising vectors for long‐term gene transduction. These viruses have a high degree of safety, making them ideal for gene therapy applications. Significant progress has been made in improving rAAV vector production and purification. In this webcast, we present a study exploring different chromatography workflow solutions to purify rAAV8.
Experimental conditions and data will be presented on using anion exchange (AEX) and mixed-mode chromatography to purify rAAV8.
Key takeaways:
- Increased efficiencies using AEX for capture
- Mixed-mode chromatography used to separate empty capsid from full capsid and remove DNA
- More flexible, economical purification workflow compared to traditional purification process
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