Lambda Phages as Vectors

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[@juandiegoariasmantilla7566]

yo' Bro. you don't know how much your videos have helped me to study for my molecular biology tests. I visit yoursite whenever I have a doubt. Thanks a lot.

[@rehamkhj3457]

I am a master student, and this helped me a lot to understand what actually I have to do in my project. TANK YOU

[@emmettfrancis9894]

Instablaster

[@bulungubenard4765]

Thank you so much Professor, it was fully packed with the clarity I was hunting for !! May God bless you abundantly for sharing your knowledge freely !!

[@ΚαλλίαΣότα]

Such an interesting and detailed video. I study Pharmacy and this will definately help me at Pharmaceutical Biotechnology's exam. I would like to give you an idea for another video: cosmids used as vectors, concatamer creation and the help of the enzyme terminase for the packaging at phage λ heads. Keep spreading your endless knowledge!

[@harleydafs]

excellent! i wish i could have a professor like you! :3

[@shivanshsurana3298]

Very neat and precise explanation. Thanks for uploading the video in HD.

[@maiyusuf6460]

very simple as well as more detailed , thanks prof👏👌

[@rawanxy7396]

thanks this is really helpful better than reading my biochemistry book. God bless you brother thanks

[@jaiekarve1424]

YOU ARE AWESOME! Thank you so much for all of this hard work!

[@jasminecassis8881]

You explain things so well! Thank you!

[@AKLECTURES]

Jasmine Cassis you're welcome :)

[@pakshanail9185]

excellent lecture thank you

[@medicogirl1783]

This was so amazing! Thanks a million for all the hard work you put in! Your explanation was precise and neat and perfect! i'm really grateful :) Sir do you have a video on lac operon? Please do share the link

[@fatvolo]

0316 uh, z as s km. N

[@qureius494]

Sir, means video is only about amplification of viral + gene of interest What about expression

[@odeniyiesther7161]

I wish one can give a love on KZbin. Your videos are mind-blowing. Thank you very much and God bless you in Jesus Name 🙏 ♥️♥️♥️♥️♥️♥️♥️♥️♥️

[@jasminekomaltamang4029]

Thank you so much 😊 AK Lectures .....you explain very well...🙏

[@nasuhanasir5817]

Thanks a lot. You really eased my genetics revision a lot. 👍🏼

[@sofiyakhan20]

Great explaination!!!

[@veerled327]

Do you have a video explaining the use of cosmids as a vector?

[@asmaaali-ln7kr]

you are more than perfect, you are my idol

[@Icareaboutyourapple]

honestly feel the same

[@geezee6064]

thanks alot it helped me for my assignment

[@darkmatter1900]

You explained it perfectly, so many thanks :D

[@fernandojdejesusrodriguez841]

E hmm

[@dinamarwan5735]

can i ask from where do we get a paspaper mcq for this subject ????

[@noname-nf1kk]

Thank u 😄 u saved my exams 😅

[@summora1990]

Thank you 😇

[@lamia9466]

this is the greatest explanation video i have seen ever, thank you

[@bdsailo8734]

Sir, in lysogenic cycle after the bacteriophage ___________ the viral , whats the blank

[@TheRockaleo]

Thank you!!

[@esthermari1694]

Great video, thank you!

[@aiswaryas2978]

Excellent tutor😍😍😍

[@Almogabil]

You safe my time , thank you

[@addu_world]

Very clear.Thank you so much.

[@halamohamed4646]

Excellent, thank you very much

[@HomeschoolSurgery]

yes, but if I want to get the proteins which the inserted gene codes for, won't the viral dna have to be transcribed and translated within the host cell? In that case, does it really matter if the phage follows the lysogenic or the lytic cycle? (that is, if it gets incorporated OR replicates right away, in which case i suppose it will work much like a plasmid?). Are the terms lysogenic - lytic cycle even used in this case? Is there any danger for the host cell either way? OR is the phage used as a vector only in the case I want to create a genome library, not a cDNA library, so I only want it to go lysogenic? (genome library--> just study the genome, cDNA --> to receive polypeptide chains)

[@debahutibhattacharjee7095]

Wow.. thank you so much😊

[@Experimentswithaftab]

Hlo sir.... U r outstanding sir.....much love sir 😍 😍 😍 😍

[@Dignifiedsan]

when virus undergoes lytic cycle??under favourable or unfavorable conditions??plz reply

[@dj_keyzofficial]

Occurs through UV INDUCTION (DNA damage), initiating an SOS response which will then cause cleavage of the C1 protein, turning on the Lytic Cycle

[@adityamadavi7901]

Whenever it utilize all the energy and protein of host cell then lysogenic cycle comes in more favor to lytic cycle...

[@thanarithanika2229]

The DNA inside the phages having 3 segments,if the segment 2 is taken out and our gene of interest is located in case of this the proteins which are produced by the segment can't produce inside the virus.Is this can't create any issues???

[@adityamadavi7901]

Out of 100% only 40% genes are useful for the lambda virus genome... Which is mostly cro/ci (useful genes) present left and right side of the genome

[@anonymous672]

you are fucking awesome! thanka you not for only this video but for allll of them.

[@manpreetkhalsa07]

also deliver a lecture on phagemids and cosmids

[@yerkozlatar2048]

to basic, its for five years old kids? como on, we have to much level in Argentina

[@andersnelson6464]

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[@sudebahar8778]

As a first-year med student, I love you and your lectures!!! Awesome, life-saving!!!!

[@CheessePie]

Thanks! very useful. How do you insert the recombinant DNA into the phage again?

[@PrinceofHope1]

Are we supposed to remove the genes involved in the lytic cycle of the phage for the purpose of cloning our DNA into E.coli cells? Is the vector then still viable for the gene to be inserted into E.coli DNA?

[@maiyeshaekra6908]

I struggled the whole semester with this! Thank you so much!

[@tomwinkler294]

can the viruses go to the lytic cycle after we replace their DNA with our DNA of interest?

[@logangrimnar3800]

I will soon be able to gene edit my tissue in vivo. Then we'll see what ubermench look like.

[@ridsbutterfly9850]

Thanks for making it really easy topic..

[@soumyanetrapal2399]

i just realised he sounds a lot like sean evans from hot ones

[@royaltayyab7354]

Awesome teacher

[@apekshadandi7767]

Thank you 🙏

[@Nangyall]

Masha Allah Allah bless you

[@sawairagul251]

Well explained

[@Manoj-MRM]

👍

[@alexaneroire5426]

hello!, I have a question.. at the end you say that we insert the fragment of recombinant DNA into the lambda phage, but where does it come from (the lambda phage)? has there been previously transcription and translation of phage DNA to make the capsid, if yes, so we need the material necessary for transcription / translation in the medium, it would therefore be necessary to add DNA polymerase, dNTPs and ribosomes, right?

[@sahanahn19]

Ur explanation is just amazing sir. Understood it in a very better way. Thank you

[@JosephineEbejerGrech]

thanks for the explanation

[@sannanielsen4728]

I had searchd a lot for this lacture...very usefull n well xplaind 👍

[@jasminecassis8881]

You're so cool

[@AKLECTURES]

Jasmine Cassis thanks Jasmine :)

[@asmataibi8051]

Perfect just the board not clear to take the notes thank you

[@peermohmmadjogezai3501]

outclass explanation great sir!

[@happyheng5476]

thank you for the great explanation!

[@afortenye9570]

get all details well explained thank u sir

[@vinaysaras]

Interesting and simple

[@klarasmolova8791]

Awesome! Thanks!

[@sukhmanpreetkaur7951]

thank u sooooooooo much sir😇

[@royaltayyab7354]

Thanks a lot dear sir🙂

[@Dolei]

what is that accent??

[@juheinaalrahbi3415]

You are amazing!

[@khushil.5650]

Thanks a bunch!

[@meroprincess5834]

thanks alot

[@junczhang]

keep up the awesome work!!

[@wenkaizhang5299]

This is really helpful. may I ask, what is the advantage of lambda vector compare to viral vectors? Does it allow bigger DNA fragments (e.g. 1Mb) to be inserted?

[@adityamadavi7901]

Not possible... Coz size of lamda phage is around 40k to 53k... For 1 mb u can use YAC( yeast artificial chromosome)

[@wongzhihong3338]

great video i have a question: how to insert recombinant DNA into lambda phage?

[@alexaneroire5426]

i think it's like in lytic cycle, so there is an encapsidation of recombinant DNA into the capsid

[@winstonl4573]

How do you remove fragment 2?

[@mimibriya2655]

restriction enzymes

[@winstonl4573]

Yes, you need restriction enzyme digestion to cut between fragment 1 and 3, leaving 2 without attaching to the sequence, so how do you remove 2 before inserting the target DNA?

[@dominiqueradtke1262]

Thank God i found your channel, now i feel prepared for my class test tomorrow :)