Restriction Enzymes and Recombinant DNA

[@jennifersobolewski4765]

I wish I discovered your lectures earlier! Very good stuff!

[@tejasvisakhamuri1380]

And I see this 4 years later

[@soggyboogy]

​@@tejasvisakhamuri1380 And I see this 4 years later

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You really are a good teacher. Thanks for doing this!

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I just gotta say, this is great. Im a non native english speaker with adhd and your lectures work unbelievably well with my brain. Thank you!

[@pooregyptiancatsanddogsshe5249]

DONATED !!! Today I passed the final exam successfully in the subject of “ Horizontal Gene Transfer “ in medical school because of your video lectures !!! I can’t find the words to thank you honestly!!! Without you , I would have been lost or failed!!!

[@muhammetmemon6963]

Andrey, really appreciate your videos; specifically writing down the sequences and showing with different colors really makes it easy to understand. Thank you very much!

[@scottbrown5478]

honestly just made everything so much more clear for me... no idea what any of this meant until watching this thank you !

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How did I not find you before? You restored my faith in teachers. Absolutely brilliant.

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You are absolutely amazing! You are saving someone whose in Egypt right now. Didn’t understand anything from my teacher, thank god you exist!

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The same I also didn't understand my lecturer i am from Kenya 😂😊

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I am truly have a crush on you AK. You are better than any professors i ever had. This is how you teach, not reading off the slides to your students.

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I'm seriously mind blown now..like all these were really confusing but after hearing this lecture everything connects and makes sense..Thank you so much

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Thank you so much for creating this channel. Your videos are helping to get me through biochemistry.

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Absolutely LOVE this video. Thank you for making hard topics easy to understand! You make studying for the MCAT a lot easier.

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Thank you! I keep reading my book and looking up each section in your videos because I can't understand my book. Thank you for your great illustrations and repetition. You have a gift.

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you have seriously gotten me through pre-med . i owe you my life

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Using this exellent videos of you , i can make more effective my studying and it takes to me less time to clearly understand things. I just wanted to thank you for your contribution to community, especially to students!

[@saraforoutani6511]

I go to school in Iran and English is my second language, but i understood this lecture more than i learn while reading my own biology book! Thank you so much!!!

[@kenm2595]

how is the coronavirus

[@joaocarneroguedes]

Greetings from Brazil! Your lessons are really very good! It seems to be easier after that, and you have a perfect "blackboard". Thanks a lot!

[@AKLECTURES]

João Carnero Guedes Thank you! glad to see they're helping you! :)

[@karunajogad390]

Best lec. of biotec ❤ all the concepts becomes crystal clear. Thank you sir☺☺

[@noorclark2226]

Without your lectures many concepts won't be that clear so thank you so much for effort

[@fakte.interesante]

I hate genetics but you are the best lecturer i have ever heard. Loved and understood each of your videos 😀 Thank you!

[@agnieszkalitomska3483]

I wish every lecturer would give the information in such an easy to process way! Thank you so much!

[@keertithekesharwani8250]

Still amazed!

[@makulmahato8663]

The ligase can rejoin the original DNA strands as well as it can form recombinants, which can be identified and separated by some techniques. To avoid self ligation of the DNA molecules, an enzyme called Alkaline phosphatase can be added to the DNA fragments before ligation. This enzyme removes the 5' phosphates from the DNA segments. Now the DNA fragments can only attach through h- bonding between the sticky ends of the DNA molecules. Inside the bacterial cell The DNA repair systems join the single stranded nicks. The recombinants can be purified later.

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I love your lectures. Glad I found these. I do recommend using a different colored t-shirt though. White on white is jarring to my optical nerves.

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Everytime I search for my topic you come up...What DON'T you know P.s you're a great teacher!

[@alhamshaheen8434]

Wooow....it is explained so clearly and the explanatory diagrams are best .Great teaching sir..Thanks!

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Amazing..I was skeptical at first but u did it for me..youre a great teacher..thank you

[@AKLECTURES]

+nick smith thanks for giving it a shot! :)

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great lectures sir..u always do well😀👍👍

[@emanatelove]

*skeptical

[@wiqarkhan786]

excellent way of teaching .....that was amazing .....now my concept is crystal clear thnx sir.....

[@Dira091998]

thank you so much for this video! i have a genetic recombination midterm exam tomorrow. This video really helped me :) wish me luck!

[@bushrahussein]

I adore the way he lectures

[@AKLECTURES]

bushrahussein thanks! :)

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THIS IS SO HELPFUL

[@danielsarhan6982]

You are amazing! Thank you so much @AK LECTURES Keep up the good work!

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You ARE my professor!

[@opiodaniel452]

Good lecture

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very help fully

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This was so helpful, and very well explain, thank you so much you saved me from getting a bad grade on my exam

[@khamisfarraj5738]

What amazing explanation dude Thanks alot.

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this awesome! am really enjoying all of your class lectures, keep it up sir.

[@naeem587]

Amazing video, great explanation. I don't understand why anyone would dislike this lol. Thanks!

[@Informationcornerchannel]

Very clear lectures thnx ...AK LECTURE

[@enwurunkechiveronica4669]

Very interesting, The lecture is well digested, thank you.

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Thank you so much.. You just saved my life!! You're really a great teacher

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Quite impressive!

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I fall in love with everything in ur video :) god bless u💕

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Tabarak Ali is it clear to you

[@faraha2285]

farah baqer haha! Omg ... that comment though!

[@kluaywael]

Thank you soo much for the explanation🌻🌻

[@shazbazification]

Great teaching. Thanks very much.

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Love your lectures...thank you.

[@thetoastwithnutella]

This make sooooo much more sense!! Thanks man 👍🏼👍🏼

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Saviour, thank you!

[@Vanessa-dg2ei]

You're just infinitely great. Thank you a lot.

[@gabriellobo3456]

Thanks mate, you're video's are very helpful

[@thuthuyle5876]

What should I do if the new plasmid does not contain the DNA fragments of interest ? T-T

[@user-tq7mq7hv4n]

woow ,verrrrry gooooood ,iam biology teacher from KSA, thak u .i relly nedded this

[@AKLECTURES]

123 4 awesome! :-) good luck with your class!

[@ayahope1636]

when these restricrion enzymes cut the phosphodiester bonds, whi will cut the hydrogen bonds to separate the two strands of DNA? And thanks sir

[@Misstigrine]

Big thank you from France! :D

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thank you, you are the best

[@hottcuteness]

This is so interesting!!! I had no idea of how useful genetics could be :)

[@sajaybrown991]

Pretty detailed and very informative. Thanks a mil

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amazing

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awesme teacher.... well understood

[@irule11846]

I'm assuming PCR is the other method of amplification for longer portions of recombinant DNA?

[@majetysampathkumarsowmyaja2148]

Thanks a lot

[@mingjuncao6390]

THANKS FROM NZ

[@atharvigupta4250]

god bless you!

[@timothymilsom1392]

Thanks so much!

[@nafimd8676]

Amazing teacher !!

[@khandakershadia6462]

After adding the enzyme ligase why don't the sticky ends of the same circular DNA reunite?

[@michaelfedorovsky7944]

Had the same question

[@MustafaRida86]

Great job.

[@tmath6861]

Dude this is really good. Thx man.

[@AKLECTURES]

T Math thanks! :-)

[@viktorholicek8201]

Thanks for the videos AK

[@AKLECTURES]

+Viktor Holicek you're welcome Viktor, thanks for watching

[@bikidas5029]

why it cuts only between aa bp?it can also cut between ag or gc bp

[@zienanema4691]

You are agood teacher

[@pedroaragon3435]

Awesome job. keep it up!

[@cyrinearfaoui6953]

I'm a bit confused here so excuse me if my question is stupid, but in case we want to obtain genes that could be later used for medical purposes, we have to make cDNA first out of humain genes and then insert it into plasmids, right ?

[@Moll662]

Good vid!!

[@Iinachanel1973k]

Extremely helpful in understanding restriction analysis. Thank you so much! xoxo

[@a.b.5473]

this was so helpful omg

[@dwill1804]

This camera quality is better than my own eyes holy shit

[@caterine4604]

Nah seriously THANK YOU

[@nitishakalia8166]

thanks a ton!!

[@taghreadshaker9376]

thank u alot . i have question . what is the type of bonds which ligas enzyme work on it to connect between two stikey ends ? thanks again

[@imagineabout4153]

Taghread Shaker phosfodiesther bond

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thank you!!

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I love you

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Thank you soo much!!!

[@AKLECTURES]

Rosa Munoz thanks :)

[@bhargavsisir]

Well..i.understand that the enzyme breaks open the dna strand between A and T base pairs in a dana molicule of a palindrome sequence ..but during a dna recombination what if the othr molicule did not have any palindrome sequence on the whole...or if it did not the the same palindrome sequence as the first one...can recombination take place during such conditions??...can such conditions arise in reality??....nd yet anothr doub of mine is that ..will the cut in a palindrome sequence take place only betweeen A ND T pairs?? if yes .then taking the palindrome Sequence as A T A T A T now where can the T A T A T A Cut take place

[@bhargavsisir]

Sorry make that A ND A not A ndT

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Sagol imena raga shen da raga dodo kezeli magrad xsnis dzn

[@manushoganyan6302]

Thank youuuu👍🏻👍🏻👍🏻👍🏻

[@frostythefairy]

I am in 8th grade Am I supposed to learn 😢

[@Cocobanana44]

please speak with more pauses :)

[@tshepangkhahlu4076]

This is the best biotechnoogy lecture series I have ever watched.

[@deeep124]

Which enzyme cleaves hydrogen bonds when the restrictions enzyme cleaves phosphodiester bond , to separate the DNA segment ?

[@therealest8574]

Ur excellent i wish u were my biology teacher

[@AKLECTURES]

Omar Abouraya Thanks Omar! :)

[@shalinibasu98]

same here 😔

[@satbiology9890]

thanks a lot very well understanding..but I have a question, we say that bacteria use these restriction enzymes to digest the viral dna when infected by them and before doing that uses methyl transferase enzyme to protect it's dna from these restriction enzymes...my question is, are these enzymes always present in bacterial cell or they will be produced up on infection??? is there any mechanism for that ?? best regards

[@pooregyptiancatsanddogsshe5249]

Mustafa Fahmi انا كنت هسقط اقسم بالله لولا الفيديوهات بتاعته !!! رهيب نجحتي فسنه اولي و سنه تانيه طب فمواد البيوكمستري و المولوكيلور بيولوجي و الهوريزونتال جين ترانسفير !!! عبقري !!! يستحق فلوس الدراسه مش الجامعه و الله

[@sohayusuf516]

I just love your way of teaching and especially the content!

[@MartianSA]

Great videos! Your teaching style is exceptional! They definitely clear up many of my questions in my readings. However, I did have one question pertaining to this. When you say that DNA ligase is added to combine the two sequences of DNA and form the recombinant DNA, how do you make sure that DNA ligase doesn't just form the two original circular forms of DNA?

[@isiherrera5408]

Shayan Alam i have the same question

[@natureetsimplicite3358]

I think the DNA Ligase will join the DNA through reforming the bond between the 5′-PO4 coming from the insert and the 3′-OH of the plasmd

[@frenlyneybur7544]

I am probably too late to reply but... A phosphatase comes to take dephosphorylate the plasmid (so it doesn't reanneal) When the gene if interest comes along, it binds to the plasmid by its phosphates.

[@Ghada-mv2en]

why can't we use PCR for amplification?

[@eunseocho461]

I dont understand

[@DR-kh9bl]

BEST explanation with details EVER, you really understand what your are explaining. Thank you, we needed you.

[@JaLLaM86]

Very nice video, thank you :) I think there is a mistake though. The 5' and 3' sticky ends do not match in the upper left drawing and lower left drawing.

[@alrunirene5896]

One thing I don't understand.... What makes the sticky ends of the two molecules bind to each other rather than just close their own molecules again once the DNA ligase is in the mix?