Amino acid charge at different pH and amino acid titration calculation problems

Amino acid charge at different pH and amino acid titration calculation problems - This lecture explains about the amino acid titration curve and this will teach you the process of calculating amino acid charge at different pH along with pka values and it will also help you to know the net charge of polypeptide chain from the pka values of amino acids.
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Thank you for watching the video lecture on amino acid titration curve and net charge problems.
This will also teach you about the amino acid charge pH and Pka values and the way to calculate net charge of amino acid.

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[@nephronum]

Can you explain why did you put carboxyl group in ionized state, whereas amino group was not ionized.(in cell conditions both are ionized)

[@pntdnails8835]

just got out of COVID quarantine and my prof didn't explain this at all and the figures were making it really confusing. I skipped ahead to the part I was confused and it took literally a minute and I completely understood this topic. thank you so much for this video that you created like 5 years ago

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[@hasankuliyev4211]

is net charge would be -2 at pH 12 since there is already -1 (pH 12 does not change -1 and -1 stays -1) in carbonyl group and very basic condition(pH 12) rips out H from NH which makes -1 as you mentioned, So -1 + -1 =-2 isn`t it?

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[@Ceeceelu_]

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[@israthossain6012]

For Glycine when pH is 12 how does the net charge is -1? In N-H because of the deprotonation the charge is -1 and in COO- bond there is -1 charge so the total net charge is supposed to be -2. When pH=7 you are counting the charge of COO- is -1 to get the total net charge. Then why when pH=12 you are not counting the charge of COO- group -1 charge to get the total net charge? Can you please explain it?

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Too good explaination and easy to understand

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[@akankshabhatt3357]

I had a hard time trying to understand this simple concept and finally it all made sense. Bless your soul for making such content, you are helping so many people including me to understand basic fundamentals in biochem/biology/chem.

[@janvimyadventures1310]

Sir I have some confusion with glycine formula. In the video it is NH - CH2- COO-. Is it correct.

[@soumyajitdas6686]

Yrs

[@raviphougat2474]

Excellent sir God bless you

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[@dailydevosinc.4345]

you've enlightened my understanding better. Thank you very much SIr

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[@bishwabhusankundu2385]

Are you sure that the pI for glycine is 7 and not 5.97...🤔🤔 Isn't pI supposed to be calculated as (pKa1+pKa2)/2 For glycine it would be (2.34+9.60)/2=5.97

[@Rohit.Kashab]

exactly as we will get zwitterion in between these two ph's so he is wrong on this part

[@makayla1774]

You made this so simple, thank you.

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[@thisariupamalika9924]

Thank you, Sir. Your videos are really helpful for me to understand the concepts of biochemistry. And I'd strongly recommend to anyone to watch your videos to clear their doubts.

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[@vishnuparihar5445]

Ok

[@vishnuparihar5445]

Thisari Upamalika ok

[@rachna4140]

sir can u make video on longpolypeptide chain like this. plzz soon becoz it gives idea how can calculate

[@sanjaisrao484]

Excellent explanation

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Thank you

[@sanjaisrao484]

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[@Empress426]

Thank you for giving me some clarity on this topic, I am currently taking a self-taught biochemistry class with a very confusing professor. Thank you.

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Your explanation is too much good sir ♥

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[@ankitaarvindbhai1059]

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[@somnathchanda1250]

will de-protonation of amine happen at pH 10 for developing a negative charge over -NH gr.

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[@shomusbiologyofficial]

Thank you

[@Kattiling]

I don't get why the net charge of pH 12 is -1. Can someone explain why? I thought it would be -2, this is because there would be a negative charge from both the nitrogen and oxygen so (-1+-1=2). I don't understand why he ignored the charge in the oxygen in pH 12, but accounted the oxygen's charge in pH of 7.

[@1rrrrrrrrrrr544f]

Eventhough there was a mistake,but thanks because you made me sure about my idea! I concluded the same thing when I studied amino acids alone!

[@enashaswa613]

where can i find part 2? it's not shown to me ,if someone could put its link i'd be thakfull

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[@surajbhowmik4404]

sir how to find the next class?

[@farooha.s8411]

Thanks you so much!! Where can I see the second part of the class? I need to know how to calculate the net charge for a polypeptide

[@ranarajeshwariba1120]

Sir me apke video se hi hamara pura syllabus ache se samaj me aa jata he so tnx and vert good work and very helpful for our study

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Thank you!

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[@misbashabir2063]

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[@rudraseth5419]

Sir could you plz elaborate how the molecule are protonated and deprotonated .

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Anjali km yes

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[@meenublossom6860]

Awesome lacture... 👌👌Thank you so much for clearing my concepts with amino acid charge calculation 🙏🙏😊

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Oh! what an explanation

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You saved meeeeeeee thank youuuuu watching this video will help a lot of embaressed biochem students because of their stupid professor

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[@suro-mallar8031]

In case of titration of a peptide chain, should we start from the fully protonated form of the peptide or the fully deprotonated form?

[@rajwinderdubb3222]

Omg Thank you so much sir! you explained it very well

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[@ivanjurkovic9088]

Good afternoon. How many moles of NaOH per mole of histidine should be added to increase 6.5 to 9 pk are given. What will be the charge of histidine at the given pH values, how will it move during electrophoresis? Thanks in advance for your answer.pk1 = ¸3.1, PK2 = 6, PK3 = 8

[@omethheenkenda2793]

thank you

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[@adnanfaisal1085]

i have a question regarding pH 12. Amine already has no H+, so why did u say deprotonation and write -1 on top?

[@somyaagarwal627]

thanks

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[@20tejas]

please make a detailed video on thermodynamics of protein stability and vant hoffs equation

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+20tejas okay. Will try to do that

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[@YashSharma-ce1hk]

Sir, I'm a JEE aspirant and I recently faced a problem on Biomolecules The question had three phases of glycine where [A]- Both acid part and -NH part was protonated [B]- Only the -NH part was protonated [C]- Both the parts was deprotonated pKa1=2.3 pKa2=9.7 The question asked if at pH=2.3, half of [A] is converted to [B] or not? So sir, can I consider it to be correct that yes it is converted, on the pretext that half part is protonated and half is deprotonated? (I am having confusion in the part where it asked 'converted', and I am not sure that half protonation would result in half conversion of phase [A] to phase [B])

[@umaverma9437]

The pI of glycine is written as 5.97 in books. Is that correct if we take ph 5.97 instead of 7 in this calculation? In books the formula for pI is , 1/2 (pK1+ pK2) i.e., 1/2 (2.34+ 9.6) = 5.97

[@aradhanagangwar7429]

Here in the structure of glycine why we are not considering NH3+while for COOH we are writing in ionized form

[@kashifazaidi3885]

But nh3 was already protonated ... Charge at pH 1 should be 2 ... Plz correct me if I m wrong

[@rony12007]

An amino acid residue side chain is deprontonated at pH9.5. What is the pKa of this residue if it is fully deprotonated at this pH?

[@skp-official]

Your videos helped me lot in class 12

[@randhirkumarbt]

pls make a video to calculate the net charge and PI (isoelectric point) of a polypeptide at different pH. Problem N0.- 11 of Lehninger biochemistry, chapter -3, 4th edition

[@suvosreemukherjee6868]

sir why only pH 7...It could also have been any value above2.3 and below 9.6..in that case also the charge would be zero i guess..??

[@Rajkumar25679]

Tqsm sir ❤️

[@shomusbiologyofficial]

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[@luthfimuhammad3068]

Can I ask a question? Why in different amino acids the carboxyl and amino group's pKA is different?

[@shomusbiologyofficial]

Because their chemical nature is different with different groups

[@she_is_a_hungry_raccoon]

let me buy a beer for that man

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Let it be

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He taught the subject better than my professor

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[@abdulharrison4380]

Thank you soooooooo much Sir!

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[@vanzanmecl5166]

since the net charge is zero originally, wouldn't the net charge be 2+ after protonation on both ends not +1 since its's zero plus 2 protons?

[@Biomaths247]

sir what will be the charge of glycine at pH 4,5,6,7,8, and 9. according to you will be zero.then what is PI value for glycine. it seems all of these ph values will be PI for glycine as well.

[@juhishrivastava8725]

Very informative sir.

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[@diksha_vlogs_N_shorts]

Confused in the last one ph 12...why is it - 1? It should be -1 +(-1) = -2

[@Niksonics]

Sir ionizable R grp wale..ka video hai?

[@raginikatiyar4511]

A polypeptide amino acid sequence 'AGKPDHEKAHIL' was desolved in a buffer of pH 1.8.the predominant form of the polypeptide Will have a net charge??

[@somanshishukla6929]

Sir why at ph 12 the ans is not - 2 bcz at ph 7 we took - 1 for carboxyl grp in our calculation

[@gargi8127]

Exactly, I have the same question

[@giftnamwanja7715]

awesome thanks. God bless

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[@highrepublic3264]

thank you for this video friend

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[@kristinerosegonzales7276]

why is the charge of carboxylic acid at pH 7 is -1? why not -1 minus 1? because initially it has a negative 1 charge then after deprotonation you'll subtract 1?

[@nepalobro8788]

Thank you for nice video. What would be the net charge at pH 5.5? If the solution at pH 1 changed to pH 11, what would be net charge? and last, What would be net charge at pH 9.6 (pH equal to pka of NH2 grpup) ? It would help me in academic endeavour if I got response. Thank you again. :-)

[@sharmisthasharma3587]

Sir pls make a video on how to calculate the absorbance of an amino acid ..

[@shomusbiologyofficial]

Okay

[@dayangasyiqin2398]

great job!

[@tirathjangra257]

How to calculate the charges on acidic Amino acid and basic Amino acid at different ph

[@shonalikrishnani8775]

sir please solve the analytical part of csir net 2017.. your videos help a lot.. thank you so much sir.

[@shomusbiologyofficial]

+Shonali Krishnani I will

[@mahaboobbashashaik5219]

nice clarity

[@shomusbiologyofficial]

Thank you

[@Sahil-fo9nu]

Sir, it means for glycine pI ranges from ~3 to 9 ?

[@jcash409]

The amine group is H2N deprotonated and H3N+ protonated not H2N+

[@rabianoor1589]

Thank you so much sir i completely understand this topic🥰😅

[@shomusbiologyofficial]

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[@swechhagyanvarsha5702]

Can someone put in the link for next lecture?

[@elvishernanserrano7736]

Where I can find the second video for polypeptide?

[@hamidazizi7658]

thanks man, Greetings from Germany :D

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@cheggexpert-chemicalcalcul6426]

PLEASE GIVE REFERENCE BOOK.......WHERE Pka=PH, HOW CHARGE DISTRIBUTED HALF AND HALF PROTONATED AND DEPROTONATED.....

[@arnavdeshpande3764]

Thanks for the Video. Please check your calculations before uploading...

[@ASkir145]

Please use a mike while recording the videos. The voice is not very audible. Especially if someone is listening to them in a bit crowded room.

[@alejandrarodriguezcruz227]

When KZbin teaches you more than a top University :/

[@sambhavmishra1873]

Hello sir, i have a question whether the Pka value would be given in CSIR-net question or we have to memorize??