This video is from Pre-lab Exercise 1: Aseptic Technique and the Streak Plate Method.
Пікірлер: 39
@Dokattak4 жыл бұрын
PPE is missing. Also, you need to keep the dish lid over the dish. That prevents contamination or infection. You literally picked up the entire thing and held it wide open for the bacteria to spread.
@kimmoraes2 жыл бұрын
That's what I was thinking, though the other information is very good, I think.
@princessramonah212 жыл бұрын
do you know videos that do that in youtube? i've seen all of them lift them all the way. Thank you!
@Lemonz1989 Жыл бұрын
Best demonstration I’ve seen. Thanks :)
@laraaziz72114 жыл бұрын
Great video thank you so much but where is you PPEs (personal projective equipments)? Gloves, googles etc..?
@djhell30543 жыл бұрын
Wearing gloves while using a bunzen flame isn't recommended because you can accidently pass your hand through the flame leading to the gloves to melt into your hand. I would recommend wearing googles when you are dealing with acids and bases (during buffer preparation for example) and when dealing with pathogenic microorganisms.
@ar-ifskik3433 жыл бұрын
80% of the views are from 2020
@heanna1724 жыл бұрын
Thankyou! Really clear explained. :)
@japeshpatel94605 жыл бұрын
wait when she heats it up doesn't it also kill the ecoli bacteria on it too?
@milagrolewis88955 жыл бұрын
no if you ,cool loop
@Dokattak4 жыл бұрын
No. You let the loop cool for a couple seconds. You can test it by gently laying it on the agar and watch for sizzling. No sizzling = cool enough. You heat it up first to kill the unwanted bacteria.
@charlottehenderson85254 жыл бұрын
Super helpful, thank you so much! :)
@ayanasser38194 жыл бұрын
How can I make total fungal count in The presence of mucor ?? It makes it very difficult 😔
@AnhTran-pn3ds2 жыл бұрын
🍸🍸👍👍💛💛💛
@greenchiky46727 жыл бұрын
Why are you doing this without gloves? Isn't that unsafety?
@TheCruz2695 жыл бұрын
yo momma
@welcometocattown20365 жыл бұрын
no, not really. She never makes contact with any of the samples, using her hands. She only uses her sterile loop. The only real reason to cover one's hands in this case would be to stop her from contaminating the cultures; however, as long as she has cleaned her hands and then used a sanitizer it will be fine. As well, this is pretty much just a demonstration lab, for people to learn how to use aseptic techniques with the loop and proper use of reduction with applying streaks to a plate. I am sure, that if doing practical lab work, she would likely have a mask, hair cover, gloves, and would likely do it all inside of a Flow Hood.
@nongphounsavat37805 жыл бұрын
even this is just demonstration, but she is handle with bacteria, the best way should always wearing the glove for your protection of yourself.
@tartanhandbag5 жыл бұрын
some labs prefer no gloves to prevent accidentally melting them onto your hands, which is more of a hazard than just singing yourself a bit. obviously this is a trade off, depending on which organism you are working with. if you are working with a more dangerous organism you would not generally be working on the bench with a flame, rather in a laminar flow where you can use gloves etc.
@th181674 жыл бұрын
It's more of a hazard to melt the glove onto your skin. Also, this probably isn't O157:H7 strain of E.Coli so there's no risk of huge dangerous outbreak.
@joshgranger33783 жыл бұрын
ok so there is just a few of mistakes here. lol
@dusulodam7196 жыл бұрын
why talking will that not introduce bacteria?
@welcometocattown20365 жыл бұрын
the torch is taking care of all of that
@sadpepe79373 жыл бұрын
Indeed, you shouldn't talk in front of your open plate or instrument.
@tiagofelix22554 жыл бұрын
here form microbiology class
@atsabina.wanjala7 жыл бұрын
Great job... your safety is key that's why we have to use personal protective equipment while handling laboratory specimens.
@shuohanyang18694 жыл бұрын
who else high af rn
@KevinLeRoy12 жыл бұрын
how do you determine the "safe area" to do your transfer in around the flame ? ie I want to transfer agr to agar where do I place my plates?
@ruipingwen53243 жыл бұрын
good presentation.
@ao_365 жыл бұрын
You are a good teacher. Thank you for this video.
@krad13634 жыл бұрын
We're going streaking!!
@maksudkerluku75175 жыл бұрын
Tahnk you ! very helpfull , very carefull explanation , slow and easy !
@ApurvKumar-kf8qn3 жыл бұрын
This is the best 🙃👍👍vidios on you tube wrt streaking . Keep it up....😇😇 love from india.
@pequodexpress5 жыл бұрын
Why must the streak lines intersect?
@welcometocattown20365 жыл бұрын
Streak lines need to intersect, so that you can drag colonies from the first streaks into the new ones. Then you sterilize your loop again, and drag a little less from the second set of lines. Then sterilize again, and drag only individual bacteria (single isolated colonies) to the final lines. You don't pick up more material between lines, you use the previous lines to create the new ones. The idea, is that you pick up less and less with each set of lines, so your final lines have individual colonies of bacteria, or whatever you are culturing. That is why the first sets of lines are called "tertiary lines" as you don't use them later. When you want to sample from your agar plate, you will use the isolated colonies from the final streaks.
@bustdown31664 жыл бұрын
thanks hgk this homework got me fucked up n you cleared everything up for me 💞