Mastering Thin Layer Chromatography Technique | TLC Hands-On Lab Experiment

  Рет қаралды 19,367

BaaYo

BaaYo

Күн бұрын

Пікірлер: 108
@BaaYo
@BaaYo 3 ай бұрын
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@DaniyalKhan-gt5ol
@DaniyalKhan-gt5ol 4 ай бұрын
Ma'am please ,never stop we are with you . Thank you very much for great explanation. You are so down to earth person . Thanks a lot .
@BaaYo
@BaaYo 4 ай бұрын
Thanks so much 👍🏼
@subhalaxmi422
@subhalaxmi422 4 ай бұрын
Thanks a lot mam.......I don't have deep knowledge regarding biotechnology practicals......you are a life savier mam...
@BaaYo
@BaaYo 4 ай бұрын
Thanks 😊
@24-ankitajagtap67
@24-ankitajagtap67 3 ай бұрын
Correction as sir mentioned in video ..polar solvents and polar components move fast but mam said correctly ... The sample in polar in nature move slow because polar components have strong interaction with the polar stationary phase ,causing them to move slowly through the TLC plate And Sample is non polar ..move fast This is because non polar components weaker interaction with the stationary phase in TLC, allowing them to move quickly through the TLC plate. The reduced affinity between the non polar sample and polar components results in faster migration on TLC plate.
@chemcatalyst..
@chemcatalyst.. Ай бұрын
If you use a polar solvent as the mobile phase in silica TLC, the polar compounds will travel faster, and the non-polar compounds will travel slower. This is because: - Polar solvents, such as methanol, acetonitrile, or water, can form hydrogen bonds with the silanol groups on the silica gel, reducing the interactions between the silica gel and polar compounds. - Polar compounds can dissolve and move more easily in the polar solvent, increasing their migration speed. - Non-polar compounds, on the other hand, have weaker interactions with the polar solvent and are more retained by the silica gel, slowing their migration. By using a polar solvent, you're essentially reversing the elution order, making it suitable for separating polar compounds. Some common polar solvents used in TLC include: - Methanol - Acetonitrile - Water - Ethanol - Acetone Keep in mind that the choice of solvent depends on the specific compounds you're trying to separate and their properties.
@24-ankitajagtap67
@24-ankitajagtap67 Ай бұрын
@@chemcatalyst.. thank u so much
@MissYaminiPatel
@MissYaminiPatel 5 күн бұрын
​@@chemcatalyst.. I have a question, if solvent is intermediate ( in between polar and nonpolar) then movement of components on the basis of mobile phase or stationary phase?
@panda5818
@panda5818 4 ай бұрын
Thank you so much ma'am. Apko main bohot phyle se follow kar raha huun . Apse padhke sab topic Cristal clear ho jata hyy. Aur theory padhne ke baad practical main zyada samj ata hyy . 😊
@BaaYo
@BaaYo 4 ай бұрын
Thank You 😊
@look6300
@look6300 4 ай бұрын
Really Appreciable, Thank you so much ma'am 💙
@BaaYo
@BaaYo 4 ай бұрын
thank you . please share
@AS-ws8nn
@AS-ws8nn Ай бұрын
Thank you so much Mam and Sir....Very helpful explanation....❤
@pankajjanagal202
@pankajjanagal202 17 күн бұрын
Very nice explanation mam 🙏🙏
@annupatil5731
@annupatil5731 10 күн бұрын
Thank you 😊
@razorr1920
@razorr1920 4 ай бұрын
Apart from the great work that you been doing since past years, which i have been following you with appreciation of your work and knowledge, I would see you as the next Kiran M Shaw in your domain. Apart from the knowledge that you impart towards biotech which drives the community by engagement, I think product or service based collaborative research would be great too.
@BaaYo
@BaaYo 4 ай бұрын
This comment made my day 😊, thanks for such kind words. 🙏🏻
@rashmipandey1305
@rashmipandey1305 4 ай бұрын
Thank you so much ma'am 🙇‍♀️🙇‍♀️🙇‍♀️🙇‍♀️ Want more videos regarding techniques
@BaaYo
@BaaYo 4 ай бұрын
Thank You Rashmi, will try to create more such videos 😊
@ZainabDalvi-q3d
@ZainabDalvi-q3d 2 ай бұрын
Thats a perfect explanation thanks mam suddenly it's was very useful for me
@BaaYo
@BaaYo 2 ай бұрын
@@ZainabDalvi-q3d thank you. Please share with your batchmates
@muhhammadsaeed9879
@muhhammadsaeed9879 4 ай бұрын
Mam upload more vedio about chemistry and chemistry experiments
@divyatiwari8573
@divyatiwari8573 Ай бұрын
Thank you so much mam ❤
@aranksharoy8415
@aranksharoy8415 4 ай бұрын
Thank so much mam its just amazing session
@BaaYo
@BaaYo 4 ай бұрын
Thanks 😊
@wisewave28as058
@wisewave28as058 4 ай бұрын
Ma'am you are doing a great job.... Hume kafi help rhegi apke is video se😊 we love you ma'am
@BaaYo
@BaaYo 4 ай бұрын
Thanks a lot :)
@thepulak1757
@thepulak1757 2 ай бұрын
Informative video .. thank you so much ❤
@its_vikrant_here
@its_vikrant_here 4 ай бұрын
Thanku so much mam this is very helpful Mam i completed MSC in pharmaceutical chemistry please suggest me job after this?
@LabKiBaat
@LabKiBaat 3 ай бұрын
50 Subscriber completed. Thank you everyone.
@meenupruthi9890
@meenupruthi9890 4 ай бұрын
Highly appreciated step, thank you mam
@BaaYo
@BaaYo 4 ай бұрын
Thank You 😊
@motivational_infinite
@motivational_infinite 3 ай бұрын
very nicely explained maam.
@aleenaahmad6784
@aleenaahmad6784 4 ай бұрын
Glad to have u ma'am waiting for more such videos ❤️
@BaaYo
@BaaYo 4 ай бұрын
Thank you 😊
@hafeezulhaq9920
@hafeezulhaq9920 4 ай бұрын
Mam kamal ka smjhaya.❤ Please PCR and its types aur baki sb common use hny wali techniques ki ak ak kr ky vedioes upload kr dian. hm studenta k liy yeh bht helpful hngi.❤
@BaaYo
@BaaYo 4 ай бұрын
Thanks for appreciating, we will try to upload more such videos.
@bsbk6065
@bsbk6065 4 ай бұрын
Thank you mam...aur bhi aisi tara vid chahye mam
@BaaYo
@BaaYo 4 ай бұрын
Sure, Thank You 😊
@debashismohanty7549
@debashismohanty7549 4 ай бұрын
Thank you ma'am ❤ it's very helpful
@BaaYo
@BaaYo 4 ай бұрын
Thanks ☺️
@tahirsmedicalandbiologyvid4969
@tahirsmedicalandbiologyvid4969 2 ай бұрын
mam is k through ham lysozyme ko egg white sy separate kr skty hein?
@devkumarsamanta8507
@devkumarsamanta8507 4 ай бұрын
Awesome session❤❤👍👍
@BaaYo
@BaaYo 4 ай бұрын
Thank you 😁
@beatstore6918
@beatstore6918 4 ай бұрын
Oh damn 🎉🎉🎉 wow kitna acha samjhaya hai mam aapne😊😊
@BaaYo
@BaaYo 4 ай бұрын
Thanks a lot :)
@muhhammadsaeed9879
@muhhammadsaeed9879 4 ай бұрын
Please upload more vedio regarding chemistry
@BaaYo
@BaaYo 4 ай бұрын
Sure, we will try to upload Chemistry videos as well
@yasaswinipadala2488
@yasaswinipadala2488 4 ай бұрын
Mam..ithni aasan se samajh gaya mam..Tq..
@BaaYo
@BaaYo 4 ай бұрын
Thanks for watching :)
@Bionika.
@Bionika. 4 ай бұрын
Mam Microbiology se bhi related lab work procedure n detail plz upload kre ......
@BaaYo
@BaaYo 4 ай бұрын
Thank You, sure will try to create more such videos.
@AryaRathi-b1g
@AryaRathi-b1g Ай бұрын
Jo polar component rahega wo solute front ke jyada paas yani kam distance travel karega n kyuki silica bhi polar nature ka hai ?
@BaaYo
@BaaYo Ай бұрын
yes
@gulabsingh1621
@gulabsingh1621 Ай бұрын
How to join this program/ rupture biotechnology
@jitubhatt69
@jitubhatt69 4 ай бұрын
Madam near ftnir pr vedio banaye
@kajalagrawal02
@kajalagrawal02 4 ай бұрын
Amazing mam👏🏻👏🏻😍 plz bring such more videos.. thankyou soo much mam😊🙏
@BaaYo
@BaaYo 4 ай бұрын
Thanks Kajal. Yes we will upload more such videos.
@shivamraja9978
@shivamraja9978 4 ай бұрын
🤩🤩🙏bhut bhut Sukriya mam
@BaaYo
@BaaYo 4 ай бұрын
Thanks for watching.
@AnushaP123
@AnushaP123 4 ай бұрын
Really helpful
@ujjwal_maurya
@ujjwal_maurya 4 ай бұрын
Mam please guide me mam aapne class 12 th ke bad kon sa course ki hai 🥺 Mai ne abhi 12 th ki hai Mujhe iss field me jana hai
@aanchalgupta6139
@aanchalgupta6139 2 ай бұрын
Jo sample ko dry krna h vo hot air oven m krna h ya phir open air m
@vishantkumar8645
@vishantkumar8645 Ай бұрын
Hot Air oven
@shaifalisoni8479
@shaifalisoni8479 4 ай бұрын
Mam plz share video on transcription nd translation
@BaaYo
@BaaYo 4 ай бұрын
Please follow my new youtube Baayo live.I have created this channel for lifescience students only
@richalatu1349
@richalatu1349 4 ай бұрын
Waiting for more videos like this ma'am 🤩
@BaaYo
@BaaYo 4 ай бұрын
More to come!
@Xushdkd
@Xushdkd 4 ай бұрын
Maam aisa he kuch technique certification course bhi kijiyee❤
@BaaYo
@BaaYo 4 ай бұрын
Good suggestion, we will consider this idea.
@Xushdkd
@Xushdkd 4 ай бұрын
@@BaaYo 🩷🤗
@AstonishingBuffer
@AstonishingBuffer 4 ай бұрын
Thankyou so much Mam ✨🌸
@BaaYo
@BaaYo 4 ай бұрын
Welcome!! Thanks for watching.
@thakurankit7159
@thakurankit7159 4 ай бұрын
Wah Ma'am new avatar mein 🙌
@BaaYo
@BaaYo 4 ай бұрын
haha thank u ankit. ye avatar kaisa hai :)
@souviknandi464
@souviknandi464 4 ай бұрын
Very helpful
@BaaYo
@BaaYo 4 ай бұрын
Thank You 😊
@DaniyalKhan-gt5ol
@DaniyalKhan-gt5ol 4 ай бұрын
Superb
@rinkalgupta1373
@rinkalgupta1373 4 ай бұрын
Thank u maam,
@BaaYo
@BaaYo 4 ай бұрын
Welcome :)
@rinkiboora1399
@rinkiboora1399 4 ай бұрын
Thanku mam...
@BaaYo
@BaaYo 4 ай бұрын
Welcome 😊
@vaibhavaghao3037
@vaibhavaghao3037 3 ай бұрын
Solvents konsa he
@gajanandbairwa6033
@gajanandbairwa6033 4 ай бұрын
Ma'am Private research institute me job kaise receive kre
@shilpagawade9575
@shilpagawade9575 2 ай бұрын
Love u maam
@BaaYo
@BaaYo 2 ай бұрын
@@shilpagawade9575 love ❤️
@aniruddhabhowmick2771
@aniruddhabhowmick2771 4 ай бұрын
Mam yeh aapka khud ka lab 🧪🧫 hain kya? Have you started a new company?
@BaaYo
@BaaYo 4 ай бұрын
No, this is Rapture Biotech lab.
@k.m.chavan
@k.m.chavan 2 ай бұрын
Unknown sample hai. To mobile phase decide kaise kiya
@janak100
@janak100 4 ай бұрын
Maim,I want to learn practical and i where to learn please suggest Mera job nahi lag rha hea
@jaysinha9500
@jaysinha9500 4 ай бұрын
सर का आवाज बहुत कम है, वॉल्यूम फूल करना पड़ रहा है, 👂 फोन लगाने के पश्चात् भी
@kuldeepraj7839
@kuldeepraj7839 4 ай бұрын
🎉
@vishantkumar8645
@vishantkumar8645 Ай бұрын
Mam jis lab me record krri h usi lab me mai work krta hu😊
@BaaYo
@BaaYo Ай бұрын
yesss :)
@dhanashrijitkar6181
@dhanashrijitkar6181 2 ай бұрын
Apne to only one time hi sample load kiya h College hme 10 times load karne ko kaha tha Sahi kay h mam
@BaaYo
@BaaYo 2 ай бұрын
@@dhanashrijitkar6181 10 times kyo.? 10 different sample or one sample 10 times?
@dhanashrijitkar6181
@dhanashrijitkar6181 2 ай бұрын
@@BaaYo one sample
@patel2209
@patel2209 Ай бұрын
7:27 meto dry ke bina hi karta hu 30 second funk marta hu or ho jata he 😢
@shallowdark4185
@shallowdark4185 4 ай бұрын
Mam aapki ki sound nhi aa rhi ahi
@BaaYo
@BaaYo 4 ай бұрын
Please try again, audio is clear in the video
@patel2209
@patel2209 Ай бұрын
Me to TLC without gloves karta hu 😅
@patel2209
@patel2209 Ай бұрын
Or mere sir to direct hi spots kar dete he without any drawing 😂
@ritikpal7968
@ritikpal7968 Ай бұрын
😂
@bikashkumar-pz4sq
@bikashkumar-pz4sq 4 ай бұрын
Mam is type ki or videos lao
@BaaYo
@BaaYo 4 ай бұрын
Yes, we are going to upload more such videos.
@muhhammadsaeed9879
@muhhammadsaeed9879 4 ай бұрын
You teach very well as like you beautiful
@guillermoiturriaga7863
@guillermoiturriaga7863 4 ай бұрын
No entiendo nada ..pero estás guapa 😘😘😘😘
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