BLAST

[@vitekllc694]

Best video I've seen on this - Thank you !!

[@rupindersayal]

Great, clear explanation of BLAST algorithm! Thanks for sharing ~!

[@rowlandpettit1937]

Nice explanation. Thank you!

[@liamcheney9310]

Nice and clear explanation! Thanks for the video :)

[@abdullahgarra8477]

Life saver!

[@casual_cupcake]

Great video, well explained. This helped me a lot. Thank you.

[@richardaugimeri4728]

Very helpful thanks!

[@YAKYAKYAK90]

Explanation of p-value is not correct (P(score|random)=/=P(random|score)). It's just the probability that if your sequences were not related you would get such a score (or a higher one). Similarly, neither P- nor E-values can (without further information but in any case should) be used to classify as distantly or closely homologous - just use the sequence identity/mean score for that (Consider finding an exact homologue of a short sequence - You will not get high E-values ).

[@danielhogan1461]

Good catch on the P-value, but I have to disagree with your second point. The reason you don't get high E-values with short sequences is because there is a high chance the match occurred by chance, and isn't the result of homology.

[@YAKYAKYAK90]

Exactly. That Is what I was trying to say (I justed changed "P- nor E-values can NOT be" to "Neither P- nor E-values can be", thanks for the catch)

[@alexekeya2773]

Interesting

[@chiranjib-konwar]

what's wrong with the microphone? it's terrible.