Brain slice electrophysiology video protocol

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[@JeanneMlle]

Good video, thanks! Nonetheless experimenters shouldn't be wearing gloves when working near a flamme (4'26''), it is a serious hazard and against every lab security instructions. Whether they are in latex, nitrile or vinyl, lab gloves can easily melt on the skin and cause serious injuries

[@abyssprive5646]

Bonjour ! Je voulais vous demander, êtes-vous du milieu de la recherche ? Cordialement

[@user-lc5je4wk8k]

Would you consider this in vitro or ex vivo, and why?

[@YenewubTesfaye-zx5kv]

What is the main objective of this procedure

[@user-ew2mq4ky6r]

blind patch, awesome

[@drfloriangrossl243]

I was thinking the same thing :)

[@abond021]

What kind of dye are you using in the pipette solution and why?

[@JeanneMlle]

They say in the video that they used Alexa 633 (50uM final) to be able to visualize the neuron they recorded from afterwards (immuno-histochemestry is performed afterwards)

[@jdroyale1046]

Que chingados