YEAST DILUTION & VIABILITY! How To: Microbrewery!

[@grindunwind7836]

Your videos are excellent brother. I'm currently a brewing student, and watching your videos reinforces alot of the material and provides practical application and strategy. Good stuff here.

[@WestOz196Four]

love these video's and I love that T shirt, cheers

[@saurabhbhawsar4624]

You are awesome, really informative

[@astonthompson3649]

love the videos jasper. from working in a lab, I was drilled into closing those very accurate/high Sig fig scales between every measurement and every addition. for this purpose, I don't think it would be a large issue, but if you're finding any inconsistencies, that's an easy place to start. Keep doing what you do my man!

[@brewerylife3596]

Thanks, great advise! Definitely keep the balance in a permanent location, level balance and close the shields for most accurate measurement. These things can read you breathing. Ill keep trying man!

[@gustavomartinez7575]

Awesome as always, looking forward to the next one!! Cheers

[@brewerylife3596]

Thanks!

[@jgcorn61]

Great stuff Jasper.

[@brewerylife3596]

Thanks

[@Rampayne]

Hola Jasper , Can u help me to figure out , what is the best nutrient for yeast? Servomyces or Wyeast Nutrient? or which one do you use?

[@andreypopov9758]

Hi Jasper. Can we have a video about hops, hops utilization. How the hop addition changes on a big system compare to small Homebrew setups. The thermal mass is different, whirlpool might be different. So what's happen with hops? Thank you

[@brewerylife3596]

Great topic, Thanks!

[@alrightythen84]

I've been diluting it to 1000 and using methylene blue. Are there negatives to either of those, in your opinion?

[@ivanvanhoof7933]

love the t shirt!

[@brewerylife3596]

ha, thanks!

[@jclorden]

Jasper, what is your preferred method for deflocculating? Thanks!

[@nalaeuteamo]

What would the total cells per mL equation look like using a hemocytometer? T= 10000x5x120xN Where, 10000 is the volume of one square of hemocythometer 5 is the numbers of squares of hemocythometer 120 is the dilution factor N is the total number of cells counted (dead and alive) Correct? Thx

[@JAWSFREE]

can you provide some info on the balance? Readability 0.1g , 0.001g or further? Capacity 200g, 400g, 600g, etc, Thanks

[@brewerylife3596]

It's a Metler Toledo benchtop laboratory balance. I dont have the manual in front of me right now for the serial number. It can go to 200g with a resolution out to the 0.00001 place. I can get more info on it tomorrow if you want. Its 1000s of dollars to buy. Cheers

[@JAWSFREE]

Yes I figured it was expensive. I am looking for a cheaper alternative that's in the sub $500.00 park. It looks like something that would get down to maybe 0.001g would work for this task. What do think?

[@brewerylife3596]

Yes, go for it.

[@bobbygaglione7553]

How is the hemocytometer count converted back to grams? Is it assumed that 1ml of slurry = 1g? Thanks for all the wonderful videos!

[@brewerylife3596]

Thanks for the question. When doing a dilution by weight, volume is not used. Most dilutions are seen as volume (ml), but grams are the other scientific way to get a dilution. In the hemocytometer equation, "5 squares counted" : (Viable Cells)(5)(Dilution) / (Chamber volume in ml or grams) = Cells per gram of slurry. Then take this to Kg or Lbs needed for pitch. Doing dilutions by weight have been more consistent for me. Hope this helps, Cheers

[@katy9999978y678678]

Brewery Life could you give an example calculation if that's not too much to ask

[@nathanielkane1060]

Love the detail, Jasper! Echoing off Bobby G's comment, how does this convert back to viable cells/ml count? Awesome vids :)

[@brewerylife3596]

Thanks man! Check out Bobby G's, I just responded, Cheers!

[@brewerylife3596]

Thanks for the conversation! When doing a dilution by volume, ml should be used not grams. This way you do not have to assume a density. How is viability checked with your method? 1ml of slurry should be pipetted into 99ml of water. Then one ml of this should be pipetted into 1ml of viability stain if you want a 200x. The hemo count calculation is right if you are counting all 25 squares. This is usually considered overkill and more commonly only 5 squares are counted, corners and middle square. If doing it this way my previous equation can be shortened to (#viable cells)(50,000)(Dilution)= Cells / ml or g depending. We use the hemocytometer to tell us the density at the end in cells/ml or cells/g depending on how we did our dilutions, either pipetting ml or weighing grams. If you want to pitch by volume do your dilutions by volume, if you want to pitch by weight do your dilutions by weight. Just my 2cents, Cheers!

[@bobbygaglione7553]

Thanks Jasper. I probably caused some confusion by leaving the serial steps out. I think the hang-up for some of those that have never done by weight before (myself included) is that it seems bizarre to have a hemocytometer chamber in mL, and get away with throwing a weight measurement into it. My post above was trying to showing some basic math to show that it still lines up. I edited the top line so it hopefully doesn't cause confusion down the road. Cheers!

[@brewerylife3596]

Totally man, I think it is because what you load into the hemocytometer is pretty much water at that dilution, 1ml=1g so it all works out.

[@vikramjitsingh4538]

ur tshirt says it all.....haha

[@vikramjitsingh4538]

ditto.......

[@berner81]

Have you ever tested an automated yeast counter like this one? kzbin.info/www/bejne/a2OTp3x9rMyVhLs

[@slipknot73745]

I've used BioRad's TC-20 for years quantifying human cell lines (biomedical researcher). They should all work fine, just make sure cell diameter is wide enough. Make sure your yeast cells are not clumped otherwise counts are WAY off. You can reuse counting slides with a quick rinse.

[@berner81]

@@slipknot73745 The Oculyze one seems the most reliable, tbh...