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Hydrophilic interaction liquid chromatography (HILIC) is often overlooked as a viable technique when trying to retain and separate polar compounds due to non-reproducible results. The pain points that analytical chemists often encounter when developing a HILIC method often can be tied to the mobile phase and the sample diluent. In this episode of Trust Your Science, we take a look at HILIC and investigate what scientists need to pay attention to ensure acceptable reproducibility.
Get more tips & tricks on HILIC chromatography from Waters: • HILIC Columns Tips & T...
See how HILIC compares with reversed-phase chromatography on Behind the Science: • Behind the Science, ep...