Cell Suspension Culture // Plant Biotechnology // Plant tissue culture // Cell culture

[@akshitanayak9453]

This is very helpful and true informative channel

[@affafahmed4985]

Thanku so much sir☺ very helpful video tomorrow is my practical exam u explained very clearly it really helpful for me thanks alot😊

[@azadhindfauj6619]

Thank you so much to making a video on Cell suspension culture. I have searched a lots but you are 1st one who uploaded a video on this topic. Thank you once again for your efforts.

[@Dr.Rakesh.B]

Thank you means a lot

[@linyuu13]

Thankuu so much sir the way you explained was very clear and your efforts are very grateful and make more videos like this and make our subject easy ☺️

[@Krishyadav6668]

Tha k you sir it helped me to visualise the whole process I read in my class 12 biotechnology book 😅

[@indiasai9966]

Thank you so much sir and also please do cell viablity test and cell density test and also suspension cell extraction . i am searching so many videos but did not found , please make a video , will be thank full for so many people.

[@XploreBio]

Excellent explanation

[@Dr.Rakesh.B]

Thank you :)

[@kanchanapeeris6798]

Great 😊

[@sumeshsudhakaran4823]

Handsome teacher.....❤️👍

[@Dr.Rakesh.B]

Haha thanks for the compliment :)

[@sumeshsudhakaran4823]

@@Dr.Rakesh.B 😘😘❤️❤️😘😘😉

[@reshmabenson1812]

Sir You are doing a great job. All your videos helped me a lot for my research work. A humble request... Please do a video on somatic embryogenesis 🙏 It would help us a lot

[@Dr.Rakesh.B]

Ok

[@AjayKumar-dh5zf]

Are you also working on guava tissue culture..?

[@Dr.Rakesh.B]

No, Mucuna pruriens

[@ketkikant6046]

Great video sir very helpful and very concisely made. Sir, if possible can you also show some techniques related to cell growth measurement parameters of cell suspension cultures.

[@Dr.Rakesh.B]

Ok will do 👍

[@s.senavirathna3835]

Tnx a lot sir

[@doaahamed5458]

What is better to make cell suspension from callus or from leaves directly???

[@akshitanayak9453]

Sir during grinding, doesn't cell rupture?

[@theasoapsacademy]

I have seen so many videos. But he demonstrated excellent with practical demonstration. ❤️👍 I'm hvg agar agar for nutrient medium. Is that fine for liquid and solid medium . And also I'm hvg homogenizer which I use for nano technology nano liposomes. So can I use that homogenizer.

[@Dr.Rakesh.B]

Thank you. Yeah u can use agar agar. But for liquid medium we don't use any agar. Only for solid we use. And yeah u can use that homogenizer after sterilizing

[@mudavathpadma4976]

Plz provide many more plant biotechnology related project lab videos

[@pjarunkumar2960]

Your are super

[@Dr.Rakesh.B]

Thank you :)

[@ahlamabdulaziz6513]

What will be temperature for suspension culture as the callus already in cold

[@Dr.Rakesh.B]

Same temperature to be maintained as callus culture

[@ahlamabdulaziz6513]

@@Dr.Rakesh.Bthank you

[@ahlamabdulaziz6513]

@@Dr.Rakesh.B sir when can we add elicitors to the suspension cultures... Also is it required subculturing along with same elicitors

[@Dr.Rakesh.B]

@ahlamabdulaziz6513 yes same elicitors and same conditions

[@kalpanarautela320]

Hello sir, In case of callus culture we do suspension culture... But sir, what should we use for subculturing the plant , when we are dealing with shoot initiation invitro ... As I have tried shoot multiplication experiment in test tube, now shoot buds are coming out but there is no space for them to grow, so I'll have to shift it from tube to flask, So please help me with the suitable procedure or suggestions for that ... Thank you 🙏🙏🙏

[@Dr.Rakesh.B]

Yes, multiple shoots will come. Cut them make sure each Cut has 1 shoot and transfer to new phyta jars with same shoot multiplication media

[@kalpanarautela320]

@@Dr.Rakesh.B okay...thank you so much sir for your speedy reply... , But when should we apply root initiation hormones to the culture ... Sorry sir, but one more question is there, Do we need to tightly seal the cap Of the cultures or air is needed inside the Culture tubes ? Hopefully you will answer whenever you get time to... Thank you so much once again 🙏🙏

[@Dr.Rakesh.B]

Yes, u need to tight the caps. We also seal them using parafilm or klin wrap. It avoids contamination. And plants do not require air from outside even if you seal it tightly the air inside is enough. Plants utilizes them and creates a cycle. If you feel u have sub cultured enough of shoots then u can add Rooting hormones and once roots are grown u can take it and go for hardening. It depends on the work and ur objectives.

[@kalpanarautela320]

@@Dr.Rakesh.B thank you soo muchh sir for your kind help 🙏🙏🙏

[@doaahamed5458]

Ii this C.roseus callus ?

[@Dr.Rakesh.B]

No Mucuna pruriens

[@akshitanayak9453]

Sir , where do you study

[@mudavathpadma4976]

Sir hw to prepare ms media

[@learninghub9715]

Kaha sa hai sir ap

[@Dr.Rakesh.B]

Karnataka

[@creatodayin4924]

Sir which chemical I used in tissue culture to prevent contamination ? I don't have PPM then what thing I use

[@creatodayin4924]

@@Dr.Rakesh.B sir i watched it but what to do if I have contamination after using all sterilization techniques.. Can you suggest me any chemical to mix with media like PPM " Plant Preservation Mixture " Thank you

[@creatodayin4924]

@@Dr.Rakesh.B really really thank you sir.. I definitely take your advaice, and ya I am little bit doing thing fast and you say about the right thing... Good things take time... Thank you sir love your videos ❤❤

[@sudhakarkommu6683]

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