Chromatography 101: An Introduction to Size Exclusion Chromatography

[@j0hhnyt93]

this would be a lot better if you were not using a toaster for a mic, still good info though so thanks.

[@user-xk9gg7vi7e]

Good video, but the sound quality is so low that it becomes difficult to hear what is being said.

[@Brunovasconcelos0]

A special thanks from Brazil! Obrigado!

[@lovebunnykaz]

Does the factors affecting resolution for group separation affect mass spectrometer resolution. For example improved chromatography resolution leads to improved Mass spectrometer resolution?

[@lovebunnykaz]

***** And that my point BIORAD, I want to couple a HPLC SEC with ESI MASS spectrometer. I need to be able to desalt proteins online - so from SEC straight into the Mass spec. I'm basically trying to make them both compatible. maybe im asking the wrong question. i wanted to know how i would do this.

[@lovebunnykaz]

***** Hey BIODRAD. Thanks for you help. My project is well underway and I have managed to couple my system yayaya. I was so confused. Acutally the SEC column is used to improve S/N in MS spectra. Lets hope an A+ comes my way for my masters. ;)

[@MsTommyknocker]

Thanx for the upload

[@sthandokuhlemathebula2184]

WHY IS IT NECESSARY TO EQUILIBRATE THE COLUMN PRIOR TO SEPARATION

[@BichitaQ]

You want to make sure you have the proper buffer before you load your sample, otherwise your protein may not bind when you need it to or may bind when you don't want it to.

[@malakalyazeedi6769]

thank you very much