Classic thresholding with FIJI (ImageJ)

[@kettor]

Thanks a lot, Johanna! Your video really sheds light on segmentation. I struggled many weeks with it, even with the printed handbook of ImageJ instructions. Now its clear.

[@johanna.m.dela-cruz]

Thank you for your support, @kettor.

[@jamilkhan8462]

How can i get printed or pdf book of imageJ/fiji. thanks

[@johanna.m.dela-cruz]

@jamilkhan8462 There are several manuals or instructions online which you could print out.

[@mohammadkhashan7425]

One of your most successful video ever. Thank you

[@johanna.m.dela-cruz]

Thanks for your support.

[@user-hl9ly4wh5g]

Fantastic tutorial. Thank you!

[@johanna.m.dela-cruz]

You’re welcome. I’m glad you liked it.

[@PatriciaNerydesiqueira]

Hi Johanna, I hope you are well :) Do you know of any alternative to using Threshold in macro, so that it automatically matches the result of each image? Because each image generates different values, and there is no way I can provide a fixed value that works for the entire set of images (there are many)

[@waelhamda]

Thanks for the very useful tutorial. Can I extract x y z coordinates for subjects using this tool. Thanks

[@johanna.m.dela-cruz]

Hi @waelhamda. Thanks for watching. Yes, you can Set your Measurements to include either centroid or center of mass. When you Analyze Particles, the measurements will include the coordinates.

[@muhammadfaisalabbasi9547]

Informative tutorial

[@johanna.m.dela-cruz]

Thanks…I appreciate your support.

[@rafezon]

Master Class

[@johanna.m.dela-cruz]

Thanks @Rafael Gonzalo Gobernado.

[@yashenggao6980]

Thank you!

[@johanna.m.dela-cruz]

Much obliged, @Yasheng Gao.