Coimmunoprecipitation

[@nbent4607]

You're really an increadible person!! Thanks for all the content provided for the youtube community. Greetings from Brazil.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@spencer0600]

Thank you Shomu! Your videos are helping make immunology understandable and even fun! Keep making the world a better place :D

[@shomusbiologyofficial]

Glad it helped

[@carolynshiloh]

amazing!! I was reading a paper for a presentation that didn't really explain their western blot, super confident I'll be able to answer any questions about how it works from my prof now :D

[@Mercyforthewicked]

the bio god never lets us down

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@Hotchanna]

Clear and easy instruction and understanding. Thank you, Shomu!

[@adarshguptak]

Very good efforts, Shomu! One small correction @ 9:32 .. you have to load on native PAGE if you wish to see mobility shift of protein complex, not on SDS-PAGE

[@shomusbiologyofficial]

+Adarsh GUPTA thank you for the correction

[@Asparagus-T]

Quick question You are unable to obtain 100% pure preparations of A,B and C. However you can express these proteins in cells. Which experimental technique would you use to prove/disprove your hypothesis? Describe the experiment and the expected results

[@TS-dp2nv]

Awesome - very informative thank you!

[@shomusbiologyofficial]

Thank you very much.

[@InquilineKea]

what's the false discovery rate of CoIP? And the false negative rate?

[@tanvirahmed4373]

Thank you so much sir............................

[@shomusbiologyofficial]

You're welcome

[@InquilineKea]

does it only identify PPIs in proteins that are part of the same complex? What if they interact but aren't part of the same complex?

[@sriradhaganguli7660]

as usual.. ur videos are always helpful. U have a solution to all topics :)

[@shomusbiologyofficial]

+Sriradha Ganguli glad to hear that

[@yassinaljubory1185]

Sriradha Ganguli hi you have therd stage

[@jasontuli9036]

any videos on anyalyzing immunoprecipitation diagrams or coprecipitation

[@rabeaahsan2070]

I M NOT GETTING THE ANALYSIS OF CO-IP CAN YOU PLZ TELL ME BREIFLY HOWEVER I KNOW WHAT IMMUNOBLOTTING AND GEL ELECTROPHORESIS IS . BUT IDK HOW THESE CAN ANALAYSE THE COMPLEX OF PROTEIN PLZ ELABORATE IT.

[@danishsameer17]

how can i prepare for gate biotech

[@yanxiantan4223]

thank you so much! love all ur video!

[@yassinaljubory1185]

we prusetiar your hard woork . if you can meak cllas for phsology of microorganism about oxedation and redox thankyou sir

[@shriyasingh7817]

if we do not know the the structure of PROTERIN (say C),how we prepare antibody?

[@arpitadas188]

I don't think u need to know the exact structure to make a. antibody ... if u have a protein n u know what the protein is .. u could just easily make an antibody out of it by injecting it to rabbit or anything .. n in case u need to find the structure of the protein .. I guess X ray crystallography is the best technique to know the exact structure n orientation ...

[@swativerma6983]

What is the use of Tag here?

[@shomusbiologyofficial]

To find out the fragment

[@swativerma6983]

@@shomusbiologyofficial ok thank you sir

[@shriyasingh7817]

how we know X actually intearacting with Protein A in PULL DOWN not Y.

[@arpitadas188]

I guess if we make antibody against the protein a.. n do something like western blotting we could guess the results out of it ... from the pull down method u are assured the ppt u are getting is of x protein complex ... n later while u are using antibody against a it confirms that its a interacting with x