Colorimetric analysis/Colorimetry (Instrumentation, applications, calibration graph)

[@rogersrivasan2150]

Absolutely gripping with passion & enthusiasm. You are truly magnificent & I love every second of it! Thank you so much for the clarity Revathi

[@RevathiPurushothaman]

Thank you very much. Such a motivating comment. Thanks again.

[@suhailfsheikh7596]

Your videos help me alot Ma'am. Thank u

[@RevathiPurushothaman]

Most welcome.

[@anaghas.7338]

Why we use 'control' while doing colorimetric estimation?? Thanku for the vedio .it really helped me☺

[@RevathiPurushothaman]

Most welcome. Are you meaning blank or reference?

[@anaghas.7338]

@@RevathiPurushothaman No . In all estimations that i have done in our lab , other than the test solution (the one with unkown concentration)and blank, we used another one named control.i dont understand the purpose of using it.

[@RevathiPurushothaman]

That may the one which lies within the specification limit. It should be a known standard whose values are known.

[@anaghas.7338]

@@RevathiPurushothaman Thanku ☺

[@adityarajsinghrathor]

Dhanyawad Guruji 🙏🙏🙏

[@RevathiPurushothaman]

Thank you very much.

[@dhaneshprabhu4724]

What's the difference between a colorimeter and spectrophotometer. Both feels the same. BTW, the explanation was too good ma'am

[@RevathiPurushothaman]

Thank you. Both are same.

[@narasimham1130]

Thank u mdm..very helpful this

[@RevathiPurushothaman]

Most welcome.

[@suhailfsheikh7596]

In estimation of Copper Sulphate by colorimetric method , blank solution used is aqueous ammonia solution. So the use of blank solution is to set the colorimeter to zero and this is done since ammonia will also have absorbing property , but we are interested only in finding absorbance of Cuprammonium complex in the analyte solution. Ma'am is this ryt?? If not, what is the use of blank solution?? Thank you 😊😊😊

[@RevathiPurushothaman]

Blank solution is the solvent used to prepare the sample to be analysed. However, if any colouring agent/complexing agent or any other reagent is added to the sample, those reagents should also be added to the blank. So the blank sample should contain all the reagents except the analyte (eg, if you are determining copper, then the solution of copper salt should not be present). This is to ensure that any reagent that absorbs light at the wavelength under study is taken into consideration in the blank solution, as we are setting the meter reading to zero for the blank.

[@suhailfsheikh7596]

Thanks again Ma'am

[@hbkshivdaas8770]

Thank you so much for this Video.

[@RevathiPurushothaman]

You are most welcome.

[@habibaansari8962]

Why do we use blank before to start the practical??

[@RevathiPurushothaman]

Blank solution is the solvent used to prepare the sample to be analysed. However, if any colouring agent/complexing agent or any other reagent is added to the sample, those reagents should also be added to the blank. So the blank sample should contain all the reagents except the analyte (eg, if you are determining copper, then the solution of copper salt should not be present). This is to ensure that any reagent that absorbs light at the wavelength under study is taken into consideration in the blank solution, as we are setting the meter reading to zero for the blank.

[@ee-zm3hz]

thank you so much! was very helpful

[@RevathiPurushothaman]

Most welcome.

[@arulmozhi.m9092]

Mam can you please upload the video for HPTLC

[@RevathiPurushothaman]

Sure.

[@roserose-wr6lm]

Mam I need vitamin analysis by colorimetric method please

[@RevathiPurushothaman]

Sure

[@roserose-wr6lm]

@@RevathiPurushothaman 😊

[@muthuvel5713]

Mam I need theoretical and practical aspects of calorimetry analysis mam

[@RevathiPurushothaman]

The estimation of Fe3+ is the practical concept of colorimetric analysis.

[@sahilalibaig1278]

Wow

[@RevathiPurushothaman]

Thanks

[@muthuvel5713]

Mam where you takes this notes pls tell man

[@yogeshmetha9285]

Mam can l get the vedio on pH metry and polarimetry experiment

[@RevathiPurushothaman]

Sure. In future.

[@yogeshmetha9285]

Thanks to you mam

[@biggydx]

If I used a white light source and a spectrometer, would I still be able to retrieve a spectrum of the sample; albeit with some wavelengths being more absorbed than others. Is only applying a single wavelength a requirement? I have a broadband (white light) LED and I'm wondering if it would be viable to use in such a setup.

[@RevathiPurushothaman]

This obeys Beer- Lambert law, which is applicable to monochromatic light only. Although we use a source which can give us visible range, we need to select one wavelength at a time.

[@biggydx]

@@RevathiPurushothaman Thank you for the information.

[@iswarya9347]

Can you please tell me the Nessler's method of instrumentation and application in visible colorimeter

[@RevathiPurushothaman]

Sure in upcoming videos

[@Ninaandsom]

I want Single beam spectrophotometer theory and diagram

[@RevathiPurushothaman]

Colorimetry itself is a single beam spectrophotometer. UV spectrophotometer is a double beam. Watch kzbin.info/www/bejne/g5rKapVrndeUY80 for double beam spectrophotometer..

[@Ninaandsom]

@@RevathiPurushothaman thank u so much

[@muthuvel5713]

Pls share the links of this notes

[@RevathiPurushothaman]

I will try to share the notes in my blogspot

[@muthuvel5713]

Mam give me your notes reference

[@muthuvel5713]

Give me some reference mam for this notes

[@RevathiPurushothaman]

You can take notes from other video itself. That would be sufficient. You can refer any analytical chemistry textbook.

[@RevathiPurushothaman]

You can take notes from the video. That would be sufficient. You can refer any analytical chemistry textbook.

[@muthuvel5713]

Pls reply mam