Compensation Deep Dive - 2/23/23 with Nicolas Loof

The compensation options in cytometry have expanded significantly over the past few years. In this webinar we'll discuss the use, benefits, and interaction of AutoSpill / AutoSpread, spectral compensation, and Ye Olde compensation (naming credit to Sabine Ivinson), using FlowJo version 10.8.1. Head to www.flowjo.com/ to learn more!

[@vblanche1]

Nicolas, many thanks for this webinar. One question: when using a 10 colors "traditional" instrument, and running a 5colors panel, is it better to 1) acquire with all PMTs opened and use spectral compensation or 2) close unused PMTs and go for traditional or Autospill compensation? Thanks, Vincent

[@NicolasLoof]

Hi Vincent! Based on our testing (presented in this video), using option 1 (acquire with all PMTs opened and use spectral compensation) will give you less spread of your data than option 2 (using non spectral comp). So I you have spreading issue with your data that cannot be resolved with better panel design, I will recommend you to acquire your data with all PMTs opened and use spectral compensation. Best

[@vblanche1]

@@NicolasLoof Dear Nicolas, thanks for your reply.

[@vblanche1]

Hello again, here at kzbin.info/www/bejne/a5Ckc2Cni6qNp9U, for Positive, you use -for ex- "Size/APC-H7-A+", so you need to adjust the "APC-H7-A+" gate or is it ok to use "only" as positive the "Size" (or Clean up) gate? Thanks