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Rubinstein Laboratory's nanofabrication protocol for making holey-carbon coated grids for cryo-EM. Evaporation of carbon onto the formvar layer can be substituted for evaporation of gold to nanofabricate holey-gold coated grids. Described in Marr, Benlekbir, and Rubinstein (2014). Fabrication of carbon films with ∼ 500nm holes for cryo-EM with a direct detector device. Journal of Structural Biology 185, 42-7. Starring Chelsea Marr as narrator.