CryoSPARC Live Walkthrough

Watch the cryoSPARC v3.0 & cryoSPARC Live Launch Video: • Introducing cryoSPARC ...
Join us while we process cryo-EM data (EMPIAR-10288) using cryoSPARC Live, an all-new cryo-EM single particle analysis software system built by Structura Biotechnology. We'll walk you through the entire interface, and process this dataset from start to finish.
CryoSPARC Live is available with the cryoSPARC software platform, which you can get here: cryosparc.com/
For more information about cryoSPARC Live, visit our guide, which has in-depth tutorials and documentation: guide.cryospar...
Download EMPIAR-10288, the dataset used in this walkthrough: www.ebi.ac.uk/...
Note: The gain reference file provided in this dataset is in a file format (.dm4) that cryoSPARC doesn't support. Download a compatible version of the gain reference to use in cryoSPARC here:
structura-asse...
Website: structura.bio/
Twitter: / structurabio
Parameters Used For Data Processing:
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Microscope/Camera Parameter
Raw pixel size (Å): 0.86
Accelerating voltage (kV): 300
Spherical aberration (mm): 2.7
Total exposure dose (e/Å^2): 58
Advanced:
Flip gain ref in Y? : True
Blob Picker
Minimum particle diameter (Å): 100
Maximum particle diameter (Å): 150
Advanced:
Use elliptical blob: True
Particle Extraction
Extraction box size (pix): 320
Advanced:
Fourier crop to box size (pix): 240