Demo: Biofilm Formation

[@danouchemohammed2903]

You work in non-aseptic conditions, you open cans and tubes far from the flame

[@echofoxtrot2.051]

This isn't aseptic technique. Let's hope that particular Pseudomonas aeruginosa strain isn't an MDRO. For anyone watching, please wear gloves and use aseptic technique with a bunsen burner.

[@learn.science]

How to quantify the biofilm after experiment.? Please mention result deeply what you have observed also. 👍👍

[@moodshealer2574]

What's the name of the method???

[@user-pc9wp1ci5b]

what is in the tube?

[@Chowdhury_sayan]

Please explain protocol

[@abdullahalhabieab1050]

how can i measure these on spectrophotometer

[@HabibUllah-dv4uy]

Did you avoid the contamination while using the same tube for adding the cvd in two different strains?

[@sonaliborase5279]

Pls name of this method

[@sushmitasingho641]

Can I please know the method used here?

[@bushraanjum7295]

Tube adherence method

[@ansakhan1123]

What was the liquid in these vials

[@chandniharinair.]

I think LB broth

[@abdullahalhabieab1050]

@@chandniharinair. nutrient broth

[@benitamaryamchohan8923]

which media you have used for culture growth?

[@abdullahalhabieab1050]

Nutrient broth

[@moodshealer2574]

what is PBS? And whats the %

[@abdulazizabdunasimov8978]

Phosphate buffered saline or just PBS buffer which helps to sustain constant pH. Just google PBS buffer

[@dollysaini2005]

Why in the name of god you are not wearing gloves

[@ignaciovillalbarecuerda7432]

When you work with no dangerous microorganism and Bunsen flame (method to achieve aseptic conditions) it is not required to wear gloves.

[@alienx2486]

@@ignaciovillalbarecuerda7432 Isn't Pseudomonas aeruginosa dangerous?

[@rafamauri94]

@@alienx2486 As far as I know it is dangerous to immunocompromised people. It rarely cause issues on healthy individuals. This doesn't mean you should not be careful when handling Psenudomonas aeruginosa samples.