Determination of Crude Fiber Content -A Complete Procedure (AOAC 978.10)

[@senalpha]

I am a teacher of Laboratory Chemistry in an italian upper school of sanitary biotechnology. This is the Weende Method for the determination of crude fiber in foodstuff for human consumption and animal feed. I will do it with a 4th year classroom in a couple of weeks using your method. Thank you for your very nice and uselful video. Chemistry made simple. I often follow lessons of US universities on line. Thank you again guys, long live US and long live Italy !!!!!!!!!!!

[@MicroChemsExperiments]

Thank you so much for your compliment. I hope that you will share this platform in your circle. Stay with us.

[@VishnuVishnu-bj1iw]

Hii i.m.from India,I m working as quality control chemist of rice bran oil company any vacancies,and I m Post graduate in MSc organic chemistry (2021) passed out

[@saadbinmasud]

as a student of Veterinary Medicine this lesson helps me in Animal Nutrition course final exam.. thanks 😊

[@hafizullah9137]

A series of such videos are needed for every type of proximate analysis like protein, fat..... Thank You for the good work. Keep the Good work.

[@MicroChemsExperiments]

Thank you. Stay with us

[@foodfeedinfo]

#NDF_Determination. kzbin.info/www/bejne/n6GVfpJqrduXnrM The more readily utilizable nutrients are enclosed by the cell wall and can be grouped as cell contents. The cell solubles are extracted by boiling with neutral detergent solution containing sodium lauryl sulfate and then cell wall constituents (NDF) are separated by filtration.

[@Asmasiddique-ke8ju]

So Organized and Understandable Video ❤

[@jagadishnc7923]

Since I am in process development this video is very useful to me for Analysis, thank you❤🌹🙏

[@MicroChemsExperiments]

Glad it was helpful!

[@dr.ajaybarman7041]

Very helpful video...keep making this kind of videos.. I hope ,oneday your video will be recognised well..

[@MicroChemsExperiments]

Glad to hear this from you. Thank you Sir for being with us.

[@sirimath111]

Excellent, orderly demonstration 👌

[@MicroChemsExperiments]

Thank you

[@surendranathsingh8040]

Very good teaching. Sir Thanks

[@MicroChemsExperiments]

Thank you so much. Stay with us.

[@Chandra6E5176]

Thank you , I'm directly in last semester of my Master's after corona lockdown and needed this so much 💯 Thank you so much Guru

[@MicroChemsExperiments]

Thanks for staying with us

[@oussamasemaili9630]

Je vous remercie infiniment c'est tellement clair

[@MicroChemsExperiments]

Merci

[@monicanyamu2207]

Thank you very much.

[@jancy98]

Should we use the defatted sample for the determination of crude fibre?

[@asmahmud2315]

It's very helpful keep it up.

[@MicroChemsExperiments]

Thank you Shimul

[@SiddiqiRaashid]

I believe the sample should be placed in a round bottom flask or in your case conical flask with a condenser so that the acid or base should not evaporate. Plz look into it .

[@MicroChemsExperiments]

You are correct. The way you are saying will be the most accurate method.

[@ogubenson5098]

I kept hearing filtrate while you transferred the residue to the second conical flask to boil in the base. Please what exactly is it? Great video though, I must say. Thanks so much

[@MicroChemsExperiments]

Filtrate is what that is left on the filter paper after the filtration process.

[@drupenymphaelilly1660]

@@MicroChemsExperiments no its the residues as the filtrate passed from the filter paper into the conical flask

[@drupenymphaelilly1660]

You are right dear......no its the residues as the filtrate passed from the filter paper into the conical flask

[@rajesh7999]

You can use silicon anti foaming agent to stop the froath and also can connect water condenser to the flask for good digestions

[@MicroChemsExperiments]

NUTHAKKI RAJESH, Thank you for your comment. We should use anti-foaming agent and water condenser. Obviously it is a good practice. Thank you for your observations.

[@rajeshsiva2257]

Hi. Is fitting of condenser is necessary in the standard procedure ?

[@MicroChemsExperiments]

Yes. It will be best to attach a condenser during boiling

[@raihaha]

Thank you thank you thank you!!!!

[@MicroChemsExperiments]

You are welcome ☺️

[@ashalathaaribail3112]

Thank you so much for this video

[@MicroChemsExperiments]

You are welcome

[@asadurrahman319]

great content thanks a lot sir

[@MicroChemsExperiments]

Thanks

[@ParveenSayyad-b1f]

Excellent

[@MicroChemsExperiments]

Thanks

[@MuhammadFarhan-gm1sc]

Thank you for the great video. May I know why we need to incinerate the fiber in this analysis. Thanks in advance.

[@MicroChemsExperiments]

Ash (Minerals) is not a part of fiber. So you must subtract the minerals or ash content.

[@Kfri4486]

@@MicroChemsExperiments Hello! Thanks for the video. Can we just then get the value for ash in the sample also by doing this fiber procedure?

[@MicroChemsExperiments]

@@Kfri4486 no. You can't. You have to follow different method for ash determination. Link: kzbin.info/www/bejne/pGO0qJVuhdxrj6c

[@Kfri4486]

@@MicroChemsExperiments thank you for the prompt answer

[@MicroChemsExperiments]

@@Kfri4486 You are most welcome

[@maahii8335]

Thank u soo much sir excellent explanation 😊😊

[@MicroChemsExperiments]

It's our pleasure. Stay with us.

[@kavyaraktajihva3733]

Thank you brother 🙏🇮🇳

[@MicroChemsExperiments]

It's my pleasure

[@shankarsahu8340]

Thinks sir ❤

[@mdsoyel6847]

Nice explanation...but i have a question what are the needs of heating the sample with acid and a base?

[@MicroChemsExperiments]

To remove acid and base soluble materials from the sample

[@olaideakinwumiakintayo7295]

@@MicroChemsExperiments Thanks for your response. But I suppose the question is not why we use the hot water, which is what I think you answered, but why we use acid and base. I think that is to remove organic materials from the sample such as carbohydrate and protein

[@yuvarajghosh2789]

Is this method applicable for germinated millets

[@shinylife150]

Hot water used to wash residue is distilled water or tap water?

[@noodles8439]

I think all the water used to deal with the sample should be distilled or purer water.

[@guruprasadk3848]

Hai... I guess we need to defat the sample i.e. remove the fat content by soxhlet extraction using Petroleum ether before proceeding for crude fiber estimation... Please share your thoughts on this

[@MicroChemsExperiments]

In AOAC method, it is not said to defate the sample before coducting the Crude Fiber Test. But if you want, you can defat your sample and there will be no problem with thr Fiber Test result.

[@darshanirg215]

if we dont do the defating for spices sample then, when calculating the residue become high and the results goes high. But when do the defat then get the normal results. So is it must to do the defat for spices (>10 fat sample)

[@zijietan8953]

@MicroChem's Experiments May I know if tempe is used to determine the crude fibre ， do we need to defatted the tempe sample first by using soxhlet method ？

[@MicroChemsExperiments]

No. Defatting is not necessary

[@nainarazzaqnizami5694]

hello , AOAC 978.10 is for fiber determination in feed stuff not for food sample

[@khadeejanasrint8031]

Sir we did this experiment for our ug project by using the animal feed. but, How will we confirm that the extracted substance is a crude fiber??an animal feed containing many substituents other than fiber.??and how will we establish that the ash content is minerals ?

[@geberemaryamterefe7721]

Thank you

[@MicroChemsExperiments]

You are welcome

[@ramshatahir8860]

Nice demonstration. Kindly give me the reference from which research paper you've taken this experiment.

[@MicroChemsExperiments]

AOAC 978.10

[@aparnasahu6448]

Nice vedio, but i have a doubt on calculation part, as it said to deduct the weight of crucible with from that of fibre, but while in doing show it was shown to substracting wt of crucible with ash from that of with fibre. So what I want to know is if the formula is wrong or the values are put wrongly.

[@MicroChemsExperiments]

Formula is correct. Basically ash content is not the part of the fiber. Thats why ash wt is subtracted from the fiber wt.

[@ashrafulbizoy8048]

temperature of hot plate? do i need magnet? can i determine orange peel's fibre?

[@sarahkandolo6735]

Hey. How did you guys do the calculations? What volume is needed for sulphuric acid and what grams are needed for the pellets. Please help..

[@MicroChemsExperiments]

Thank you Sarah Kandolo for your queries. I used 3.5 ml sulfuric acid-98% to make final 500 ml of 0.128M sufuric acid solution and I used 6.25g of NaOH pellets to make final 500ml of 0.313M NaOH solution, as described by the AOAC 978.10 method. I followed the AOAC 978.10 method strictly throughout the test.

[@foodfeedinfo]

#NDF_Determination. kzbin.info/www/bejne/n6GVfpJqrduXnrM The more readily utilizable nutrients are enclosed by the cell wall and can be grouped as cell contents. The cell solubles are extracted by boiling with neutral detergent solution containing sodium lauryl sulfate and then cell wall constituents (NDF) are separated by filtration.

[@coffeegirl7426]

Thank you so much this is really helpfull..but can you please confirme the temperature of the hot air oven..u said 130° and you wrote 230°C... And please confirme the time of incerination u said 2h then u desiccated after 4h... I think it's 4h coz the T is 550°C . Thank you

[@MicroChemsExperiments]

You are correct. Temperature is 130 degree Celsius and incineration for 4 hours

[@coffeegirl7426]

Thank you so much sir

[@irhaachaudary1638]

How can we determine crude fiber? Which method use?

[@noodles8439]

I read some standards and methods for fiber determination, and I have some experience in the lab. However, I still think there are a lot of improper operations in the video, like making the solution directly in the volumetric flask even if the regent generates heat when it is dissolved in water. and for the collection of the residue, I deeply doubt that the scratch movement can collect all fiber and had no influence on the real result. besides, the white cloth can be used as the filter paper? it's too casual in my opinion.

[@MicroChemsExperiments]

Thanks for you comment. Actually this is only for beginner to get their concepts clear in a short video

[@noodles8439]

anyway, all the lab work should be done in a very cautious safety and serious way. even if it is only for showing the concepts.@@MicroChemsExperiments

[@heart_ofkkr.i8953]

Please share calcium phosphorus test procedure

[@MicroChemsExperiments]

Noted

[@user-rv3nz7fd6j]

Thank you very much, sir. Can you send me the source used for this method or the link to obtain the chemical analysis of foodstuffs because I find it difficult to obtain the source used

[@user-rv3nz7fd6j]

@MicroChemsExperiments

[@user-rv3nz7fd6j]

Is the same method used to estimate dietary fiber in rice, and can you provide me with AOAC or AACC methods with the latest copies?

[@user-rv3nz7fd6j]

@MicroChemsExperiments

[@AmenGetaye-k7h]

Please provide coffee husk fiber extraction by alkaline treatment

[@MicroChemsExperiments]

Noted

[@imim1729]

Thank you for the methode it's very helpful, just a question how can i reach the paper of the methode??

[@MicroChemsExperiments]

No. You can't. You can just take note from this video

[@imim1729]

@@MicroChemsExperiments but i need it for my research please 🙏

[@MicroChemsExperiments]

@@imim1729 from which country you are?

[@imim1729]

@@MicroChemsExperiments Algeria

[@MicroChemsExperiments]

@@imim1729 knock on our Facebook page.

[@darshanirg215]

can we use octanol as a anti bumping agent for base digeation .?

[@MicroChemsExperiments]

It is not suggested to use. Use glass bead to avoid any kind of error

[@tahabazine3397]

We put the crucible inside the Muppled Furnace for 2 hours or 4 hours ?

[@MicroChemsExperiments]

Taha Bazine, 2 hours is enough to make ash of the fiber content. So we kept the crucible in Muppled Furnace for 2 hours

[@padmaladon8739]

It showed 4 hours too... may be it was including the cooling time till it reached 250 degrees C.

[@raniyafasil7790]

In our lab, we use gooch crucible for crude fibre determination.. then what is the need of this gooch crucible instead of this type of crucible??

[@yousraboukssessa4849]

this method is valid for plants please

[@MicroChemsExperiments]

Yes

[@e-man3584]

How do you account for sample loss when taking sample from the linen to crucible?

[@MicroChemsExperiments]

Sample will not be lost if you take care during transfering. Or may be the loss of sample is not significant.

[@romeites6398]

Simple question. What kind of food makes stool soften?

[@MicroChemsExperiments]

Low fiber containing food

[@romeites6398]

@@MicroChemsExperiments For example of foods?

[@romeites6398]

@@MicroChemsExperiments I hope you reply it soon. I need examples of food

[@sirahamadi2105]

thank u very much, one thing more , i would like to ask at what temperature u adjust the hot plat for heating up the solutions thanks for answering

[@MicroChemsExperiments]

There was no specific temperature to be adjusted. We need to just boil the solution. So you can adjust temperature at 120-140 degree Celsius

[@mathiasbee3155]

Hi can I skip the oven drying method and go straight to the furnace step instead?

[@MicroChemsExperiments]

No. Oven drying and after that taking weight is mandatory.

[@mathiasbee3155]

Thank you for the reply and another last questions isit okay if I stop at the step where we collect the residue and leave it in the crucible, then we will run them in the oven and furnace after two days?

[@MicroChemsExperiments]

@@mathiasbee3155 Yes. It is okay

[@mathiasbee3155]

Thank you for the reply...appreciate it 👍🏻

[@MicroChemsExperiments]

@@mathiasbee3155 Stay with us

[@taharhanafi7928]

Thanks

[@MicroChemsExperiments]

You are welcome

[@thirulogasundharbalakamatc5254]

Can you please specify the mesh size for the cloth?

[@MicroChemsExperiments]

No. Just use cotton cloth as shown in this video

[@biancaysabelvicente215]

Hi Sir, why should the boiling be continued for exactly 30 minutes? Please help

[@MicroChemsExperiments]

Because boiling of sample for 30 minutes is needed to dissolve extra materials other than fiber, in acid and base and finally remove by filtration.

[@biancaysabelvicente215]

@@MicroChemsExperiments Thank you so much, for responsing and for doing this kind of videos, your videos are very helpful

[@MicroChemsExperiments]

@@biancaysabelvicente215 Thank you too for being with us.

[@ireshanishadi9865]

Is this method good for rice

[@MicroChemsExperiments]

yes

[@onitnelovabequibel8759]

Hello I have a question Why is the paper stained with the different test solutions in the determination of fiber composition?

[@MicroChemsExperiments]

I'm confused of which paper are you talking about

[@hebasayed-d5h]

please can i calculate : the weight of crucible with fiber - the weight of crucible only , instead with ash , yes or no and why ?

[@sumeet1769]

can i use this method for estimate crude fiber in millet rusk/toast ?

[@MicroChemsExperiments]

Yes

[@parthsakhavala1764]

Sir plant sample is taken and required a defat with ether or not

[@MicroChemsExperiments]

Not required

[@nabilaislam531]

Thank you so much for this video😊😊😊 I have a question if i want to determine the crude fiber in fruits or vagetables then how can i do the sampling??

[@MicroChemsExperiments]

Collect your sample and bend the fruits & vegetable to make it homogeneous which contains each portion of the sample in same proportion. Then from the homogeneous mixture of the sample, take 2g for testing.

[@nabilaislam531]

@@MicroChemsExperiments Thanks a lots

[@nabilaislam531]

@@MicroChemsExperiments will you tell that before determining crude fiber do i have to make the sample fat and moisture free

[@MicroChemsExperiments]

@@nabilaislam531 Most welcome

[@MicroChemsExperiments]

@@nabilaislam531 no. You dont need to remove moisture and fat from the sample. Test the raw sample as you received or collected.

[@ovaneschakoyan6584]

What type of filter paper with vacuum filtration device can i use please or exists clogging problem

[@MicroChemsExperiments]

Vacuum filtration is not applicable for this test.

[@lakhanyadav6205]

Sir please make video for urea activity check method in food all 3 method.

[@MicroChemsExperiments]

Ok we will make video soon. Stay with us

[@UnknownHakim]

May i know. What kind of this method is? For example, for antioxidant we can use DPPH method.

[@MicroChemsExperiments]

It is a digestion method

[@Kfri4486]

Hello! Thank you so much for the video. I have one question. I performed this experiment and after the NaOH digestion, I ended up without any sample, everything filtered through the cloth. Do you have any advice on this matter? Thank you!

[@MicroChemsExperiments]

So this result concluded that you sample does not have any fiber content.

[@Kfri4486]

@@MicroChemsExperiments thank you! Also, besides using cloth for filtering, can I use other material?

[@कुंवरभवनीकसरवालराणा]

You can also use fibre bag

[@MicroChemsExperiments]

@@कुंवरभवनीकसरवालराणा yup. You are right

[@itsmydayandurday]

Hi! I want to determine the dietary fiber of jam. Can I use the sample in its wet form and without defatting it? Hopefully in the future you can make videos about determining dietary fiber😁

[@MicroChemsExperiments]

Yes. you can

[@itsmydayandurday]

@@MicroChemsExperiments Jam contains very high sugar, for that is it necessary to desugar it first?

[@MicroChemsExperiments]

@@itsmydayandurday Method says that desugar is not necessary because high sugar is minimised taking low volume of prepared sample in the titration flask.

[@priyanshimartin8582]

Hey ! I forgot to do the step when you put the crucible with sample in hot air oven but I placed the crucible in muffle furnace at 130 degree celsius for 2 hours .. So what should I do ? Should I repeat the procedure or should I put the sample again in muffle furnace but this time at 550 degree for 2 hours ? Please help !! 🥲

[@MicroChemsExperiments]

Normally in muppled furnace, low temperature (100-250 degree Celsius) can not be applied correctly, because it is designed for high temperature applications. Many times we saw that, when we set the furnace temperature at 130 degree Celsius, the actual temperature rises above 150 degree Celsius. But if you ensure that the actual furnace temperature was stable at 130 degree Celsius for two hours then there is no need to repeat the test. Just take weight and make ash using furnace again doing the final Fiber calculation.

[@khadijatogunyemi6756]

Good day sir, can you please tell me the name of the sample you used? Thank you

[@MicroChemsExperiments]

Animal Feed

[@abinayas3752]

Good evening sir. I have one doubt. The wash water should be distilled or can I use tap water also

[@MicroChemsExperiments]

Never use tap water in analytical chemistry lab

[@nursuhaizamohdfauzi4409]

Can we used filter paper instead of cloth?

[@MicroChemsExperiments]

No. There will be clog on filter paper. You cant filter it out

[@bijoymaitra]

Is the method suitable for plant leaves?

[@MicroChemsExperiments]

Yes. This method is also applicable for plant leaves and vegetables

[@bijoymaitra]

@@MicroChemsExperiments For plant leaves,, should we take the weight of raw leaves directly or should we take the weight after drying in oven??? What's your idea about it.

[@MicroChemsExperiments]

@@bijoymaitra You can used raw leaves to test if you consider the raw leaves as sample. But if you wish to test the sample on dry basis then you have to dry it in over before testing.

[@Mochiindesign]

i would like to ask why we need to remove the moisture content from the sample?

[@MicroChemsExperiments]

Moisture content is considered as impurities of any sample.

[@Mochiindesign]

@@MicroChemsExperiments thank you

[@MicroChemsExperiments]

@@Mochiindesign you are welcome

[@sakirhossain2016]

Is it Molar soln or Normal soln?Cause For . 128 Molar Soln it may be needed 6.272 ml H2So4 in 500 ml water?!

[@sakirhossain2016]

Eagerly waiting for your response Dear...

[@MicroChemsExperiments]

Thank you for your patience. It should be Molar which is already given in this video. According to AOAC official method, the H2SO4 solution prepared in this video is Molar solution. If you have any confusion with it, please read the AOAC reference method mentioned in this video title. Thank you.

[@sakirhossain2016]

Thank you for your response. Request to recheck and I will try also.

[@MicroChemsExperiments]

@@sakirhossain2016 We rechecked your confusions from AOAC method.

[@khadeejanasrint8031]

Sir ,Which sample is taken for the extraction of crude fibre.will you please suggest some available fibre content sample?

[@MicroChemsExperiments]

Try animal feed

[@abebawewunetu8829]

please show protein analysis

[@MicroChemsExperiments]

We have already made video for protein determination. Please search it in this channel

[@palas987654321]

Hi, Can I able to implement this method for coconut fiber content determination?

[@MicroChemsExperiments]

Yes. You can

[@subhojitbhowmick3966]

Sir please ...I need a video for collour of oil .....

[@MicroChemsExperiments]

Will try

[@subhojitbhowmick3966]

@@MicroChemsExperiments please Tintometer collour...... Full details video

[@MicroChemsExperiments]

@@subhojitbhowmick3966 Ok

[@luisarmas2366]

If I use the fiber analysis by the AOAC 962.09 method, is it also correct? Fiber incineration is not considered in this analysis.

[@MicroChemsExperiments]

If you follow AOAC 962.09 strictly then it is okay

[@कुंवरभवनीकसरवालराणा]

Please mention Boiling temperature !?

[@MicroChemsExperiments]

120 degree Celsius

[@कुंवरभवनीकसरवालराणा]

@@MicroChemsExperiments thank you sir❣️

[@MicroChemsExperiments]

@@कुंवरभवनीकसरवालराणा you are welcome

[@Limelight530]

I have to extract fiber from potato peel kindly suggest me any procedure .

[@MicroChemsExperiments]

Zainab Shoukat, you can follow the method, described in this video, without any doubt. But before going for the test, you have to blend and grind the potato peel sample properly.

[@Limelight530]

@@MicroChemsExperiments thank you so much

[@MicroChemsExperiments]

@@Limelight530 welcome

[@zhijunlim5769]

Hi there, I have some question need for yours response. 1. The sample size is there any recommendation (base on the % of the crude fiber)? 2. Does this 2g of sample is fixed? Does the method have the maximum sample weight for the acid and alkali which is enough to digest? 3. If I increase the sample size, do I need to increase the boiling time of both acid and base? 4. 2g vs 2.5g of same sample will be have any difference in the crude fiber obtained?

[@MicroChemsExperiments]

In AOAC official method, sample size is declared as around 2g. But I think 3g sample can be used but not sure about the sample size of more than 3g. To follow AOAC method strictly, we should always use 2g sample. Sample size should not be increased considering the expected fiber content.

[@piyushprajapati6080]

Can we use magnetic stirrer to stirr the solution while heating (boiling) with acid and base? you had to stirr conical flask with hands.

[@MicroChemsExperiments]

Piush Prajapati, If you use magnetic stirrer continuously during boiling, then foam will be form and which will overflow the flask and can cause loss of the sample. If you want to use magnetic stirrer then you must have to use anti-foaming agent with the sample during boiling to prevent foam formation.

[@nursyazwani6680]

what the temperature used during boiling point in acid ( h2so4 ) ?

[@MicroChemsExperiments]

140 degree Celsius

[@nursyazwani6680]

@@MicroChemsExperiments okey thankyou ! your vid very help to success our project 😊🙌🏼

[@ramlak5034]

Do more video in species

[@MicroChemsExperiments]

Mention the test parameter

[@narendarpratapyadav9908]

Hello Sir this process use in every product of fiber let maize gluten ,dry maize ,csl mix fiber... Please give me suggestions ,

[@MicroChemsExperiments]

You can check fiber content for all of the products you mentioned.

[@PriyaKumari-ti6mi]

What does this fibre content indicate about adulteration of the cereal(sample) ?

[@MicroChemsExperiments]

Quality of a sample

[@A12966]

There is just 1 mistake in video that is temp in oven is 130 degree for two hours and it was written 230 degree for three hours

[@MicroChemsExperiments]

Oppss Sorryyy ... It was just a typing mistake

[@A12966]

@@MicroChemsExperiments ok no problem. Just tell me the procedure is this video is for those who dont have crude fibre equipment... Those who have crude fibre equipment or instrument they do not need hot plate for heating, and furnace, and etc. Pls explaim

[@MicroChemsExperiments]

@@A12966 We don't have automated crude fiber analyzer. So, we dont have any idea about it to explain you.

[@win15_13]

In determining the crude fiber content of what sample do you use?

[@MicroChemsExperiments]

Feed sample

[@Limelight530]

For drying temperature is 230* or 130* ? U wrote 230 while in the sound it is 130 degree

[@MicroChemsExperiments]

Zainab Shoukat, Thank you for your observations. Drying temperature should be 130 degree Celsius. 230 is our typing mistake and we are very sorry for that.

[@Limelight530]

@@MicroChemsExperiments OK Np

[@nadianadzirah8910]

is it can be used for liquid sample like fruit juice?

[@MicroChemsExperiments]

Yes. This method can be used for liquid sample.

[@nadianadzirah8910]

@@MicroChemsExperiments then, how much ml of sample needed for this experiment?

[@MicroChemsExperiments]

@@nadianadzirah8910 Use 5g of liquid sample for the test.

[@nadianadzirah8910]

@@MicroChemsExperiments Thank you so much!

[@MicroChemsExperiments]

@@nadianadzirah8910 You are most welcome

[@romeites6398]

Can you make experiment with real fruit or soluble fiber?

[@MicroChemsExperiments]

You can do fiber test of real fruits by this method described in this video.

[@zijietan8953]

Hi. Which year of AOAC 978.10 used in this experiment？

[@MicroChemsExperiments]

21st Edition, 2019

[@milkimojo6849]

what is the problem if we not cover crucible with it lid

[@MicroChemsExperiments]

Loss of ash may occure during burning of sample at high temperature for Ash dwtermination method. But in crude fiber test, loss of ash will not happened as the content in crucible is very small amount. So you can keep the crucible open in this test.

[@Limelight530]

This can be also known as the extraction of fiber from any sample?

[@MicroChemsExperiments]

You can use method for all of the food, feed and vegetables sample

[@vitthalnawale]

Filtration cloth mesh size?

[@MicroChemsExperiments]

Not known. Just use Cotton Cloth

[@fejankhan8248]

Sir how to use in Hot plate temprature

[@MicroChemsExperiments]

Just enough to boil

[@vitthalnawale]

Temp required?

[@MicroChemsExperiments]

No specific temperature, just boil the solution

[@pavithradesu]

How to find the fibre content in glass & kevlar fiber composite materials?

[@MicroChemsExperiments]

This method is selectively applicable for Food, Feed, Fruits, Vegetables, Environmental sample and related sample.

[@pavithradesu]

@@MicroChemsExperiments Okay sir...Thank you

[@MicroChemsExperiments]

@@pavithradesu You are welcome.

[@qiaowei230]

Hi, can I dry the samples that after treated with acid and alkaline by using hot air oven at 105 degree overnight instead of 230 degree for 2 hours?

[@MicroChemsExperiments]

Qiaowei230, This drying is needed only for the removal of moisture content from the fiber content. Drying at 130 degree Celsius for 2hours is enough for the purpose according to the AOAC method of Moisture determination. Long time drying at 105 degree Celsius could be a reason of loss of some fiber content. So you should follow the standard method to analyse any sample to get the accurate results.

[@yousraboukssessa4849]

Bonjour s'il vous plaît j'aimerais réaliser cette expérience mais on dispose pas de hotte aspirante au laboratoire. Et les produits dégagé par l'acide sulfurique sont toxique comment faire svp

[@MicroChemsExperiments]

Without fume hood you have to release in open air. No other option

[@yousraboukssessa4849]

Merci beaucoup